1型糖尿病进展中转录组范围内n6 -甲基腺苷甲基化动态特征

IF 3 4区 医学 Q2 GENETICS & HEREDITY
Wu Duan, Ziyi Peng, Kun Yang, Mengyuan Hu, Xuefeng Yu, Benping Zhang, Li Liu
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引用次数: 0

摘要

目的:绘制不同阶段1型糖尿病(T1D)转录组n6 -甲基腺苷(m6A)谱。材料与方法:采用MeRIP-seq和mRNA-seq分析不同年龄非肥胖糖尿病小鼠(4周龄对照组、8周龄糖尿病前期组和14周龄糖尿病组)胰岛组织中提取的RNA。生物信息学分析,包括m6A基序富集、差异甲基化、基因本体和途径分析。结果:生物信息学分析显示,在糖尿病进展过程中,m6A甲基化位点逐渐增加,并出现独特的峰值。在对照组和糖尿病前期,主要的m6A基序是“GGACU”,在糖尿病期转变为“GGACU/A”。m6A修饰主要富集于起始密码子、编码区和停止密码子。综合分析发现,与对照组相比,糖尿病前期、糖尿病组显示出明显的m6A和mRNA变化。通路分析表明,m6A甲基化的差异表达mrna主要富集于糖尿病前期的胰岛素/IGF-MAPKK/MAPK级联、凋亡、t细胞活化、白细胞介素、CCKR和炎症相关通路。随着糖尿病的进展,包括Wnt、EGF受体、PDGF、GnRH受体和血管生成在内的其他途径参与了疾病的发展。在体外,细胞因子刺激INS-1细胞增加m6A甲基化,上调METTL3,下调ALKBH5。结论:m6A甲基在T1D疾病进展中可能影响mRNA表达和信号转导途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transcriptome-wide N6-methyladenosine methylation dynamic profile in type 1 diabetes progression.

Aims: To map transcriptome-wide N6-methyladenosine (m6A) profile at different stages of type 1 diabetes (T1D).

Materials & methods: RNA extracted from islet tissues of non-obese diabetic mice at different ages (4-week-old control group, 8-week-old pre-diabetes group, and 14-week-old diabetes group) was analyzed by MeRIP-seq and mRNA-seq. Bioinformatics analyses, including m6A motif enrichment, differential methylation, gene ontology and pathway analysis.

Results: Bioinformatic analysis revealed a progressive increase in m6A methylation sites and unique peaks throughout diabetes progression. The predominant m6A motif was "GGACU" in the control and pre-diabetes stages, shifting to "GGACU/A" in the diabetes stage. m6A modifications were primarily enriched at the start codon, coding region, and stop codon. Integrated analysis found pre-diabetes, diabetes groups showed distinct m6A and mRNA changes versus control. Pathway analysis indicated that differentially expressed mRNAs with m6A methylation were predominantly enriched in insulin/IGF-MAPKK/MAPK cascades, apoptosis, T-cell activation, interleukins, CCKR, and inflammation-related pathways in the pre-diabetes stage. As diabetes progressed, additional pathways, including Wnt, EGF receptor, PDGF, GnRH receptor, and angiogenesis, became involved in disease development. In vitro, cytokine stimulation of INS-1 cells increased m6A methylation, upregulated METTL3, downregulated ALKBH5.

Conclusions: m6A methyl group dynamics in T1D disease progression, potentially influencing mRNA expression and signal transduction pathways.

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来源期刊
Epigenomics
Epigenomics GENETICS & HEREDITY-
CiteScore
5.80
自引率
2.60%
发文量
95
审稿时长
>12 weeks
期刊介绍: Epigenomics provides the forum to address the rapidly progressing research developments in this ever-expanding field; to report on the major challenges ahead and critical advances that are propelling the science forward. The journal delivers this information in concise, at-a-glance article formats – invaluable to a time constrained community. Substantial developments in our current knowledge and understanding of genomics and epigenetics are constantly being made, yet this field is still in its infancy. Epigenomics provides a critical overview of the latest and most significant advances as they unfold and explores their potential application in the clinical setting.
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