聚乙二醇4修饰的SVVYGLR肽在体外人牙龈成纤维细胞创面愈合模型中增强迁移和粘附蛋白的表达

IF 1.7 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Peiying Xiong, Chengcheng Yu, Zhihui Chen, Luyuan Chen, Yonglong Hong, Buling Wu
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引用次数: 0

摘要

简介:本研究旨在探讨聚乙二醇(PEG4)修饰的4个单位ser - val - val - tir - gly - leu - arg (SVVYGLR)肽(SV肽)对人牙龈成纤维细胞(HGFs)增殖潜能和迁移能力的影响以及粘附相关蛋白的激活。材料与方法:采用多肽固相法合成PEG4-SV肽。使用细胞计数试剂盒-8 (CCK-8)定量评估hgf对不同浓度PEG4-SV肽的增殖反应。通过体外伤口愈合实验评估hgf对PEG4-SV肽处理的迁移能力。采用qRT-PCR定量分析黏附相关基因,包括I型胶原(col1)、vinculin (VCL)、局灶黏附激酶(FAK)和整合素β1(ITGB1)的表达水平。为了评估上述粘附相关蛋白,采用免疫荧光和免疫印迹法。结果:原代HGFs通过酶切分离,随后对vimentin和CD90 (Thy-1)标记物具有阳性免疫反应性。与对照组相比,PEG4-SV浓度为10、20、40 μg/mL时均能显著促进hgf的增殖。20 μg/mL PEG4-SV处理后,细胞粘附相关基因和蛋白(ⅰ型胶原、血管蛋白、FAK、整合素β1)的表达均显著上调。结论:这些结果表明,PEG4-SV肽可能具有促进种植体表面周围软组织愈合的能力,并在种植体的粘膜部分形成紧密的软组织密封。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhanced migration and adhesion protein expression by polyethylene glycol 4-modified SVVYGLR peptide in an in vitro human gingival fibroblast wound healing model.

Introduction: This study was aimed at exploring the effect of four units of polyethylene glycol (PEG4)-modified Ser-Val-Val-Tyr-Gly-Leu-Arg (SVVYGLR) peptide (SV peptide) on the proliferative potential and migrative capability of human gingival fibroblasts (HGFs) and the activation of adhesion-related proteins.

Material and methods: PEG4-SV peptide was synthesised using peptide solid phase synthesis. The proliferative response of HGFs to varying concentrations of PEG4-SV peptide was quantitatively evaluated using a Cell Counting Kit-8 (CCK-8) assay. The migratory capacity of HGFs in response to PEG4-SV peptide treatment was evaluated using an in vitro wound healing assay. The expression levels of adhesion-related genes, including collagen type I (COL-1), vinculin (VCL), focal adhesion kinase (FAK), and integrin β1(ITGB1), were quantitatively analysed using qRT-PCR. To assess the aforementioned adhesion-related proteins, immunofluorescence and Western blotting were performed.

Results: Primary HGFs were isolated through enzymatic digestion using dispase, and subsequently characterised by positive immunoreactivity for both vimentin and CD90 (Thy-1) markers. Compared to the control group, PEG4-SV at concentrations of 10, 20, and 40 μg/mL significantly promoted the proliferation of HGFs. The wound area in the SV group exhibited a significantly smaller size in the monolayer cell culture at 24 and 48 h. The expression of adhesion-related genes and proteins (collagen type I, vinculin, FAK and integrin β1), were significantly upregulated after treatment with 20 μg/mL PEG4-SV.

Conclusions: These results demonstrate that PEG4-SV peptide may have the ability to promote soft tissue healing around an implant surface and form tight soft tissue sealing on the transmucosal part of the implant.

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来源期刊
Folia histochemica et cytobiologica
Folia histochemica et cytobiologica 生物-生化与分子生物学
CiteScore
2.80
自引率
6.70%
发文量
56
审稿时长
6-12 weeks
期刊介绍: "Folia Histochemica et Cytobiologica" is an international, English-language journal publishing articles in the areas of histochemistry, cytochemistry and cell & tissue biology. "Folia Histochemica et Cytobiologica" was established in 1963 under the title: ‘Folia Histochemica et Cytochemica’ by the Polish Histochemical and Cytochemical Society as a journal devoted to the rapidly developing fields of histochemistry and cytochemistry. In 1984, the profile of the journal was broadened to accommodate papers dealing with cell and tissue biology, and the title was accordingly changed to "Folia Histochemica et Cytobiologica". "Folia Histochemica et Cytobiologica" is published quarterly, one volume a year, by the Polish Histochemical and Cytochemical Society.
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