尼达尼布通过靶向NF-κB信号通路减轻支气管肺发育不良大鼠高氧诱导的肺损伤。

IF 1.7 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Rui Hao, Yuping Li, Junhui Li, Zirui Guo, Zhi Yang, Wei Lu
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引用次数: 0

摘要

支气管肺发育不良(BPD)是一种常见于早产儿的慢性呼吸系统疾病。高氧是BPD的主要致病因素。尼达尼布是一种小分子酪氨酸激酶抑制剂,已被证实影响不同疾病的几种细胞过程。本研究的目的是探讨尼达尼布在治疗BPD中的作用。材料与方法:以新生大鼠(出生后第1天)为研究对象,采用高氧诱导法建立体内BPD模型。大鼠腹腔注射尼达尼布。采用血红素-伊红(H&E)染色检测BPD大鼠肺损伤。采用TUNEL (tdt介导的dUTP缺口末端标记法)检测细胞凋亡。应用Western blotting检测肺组织中炎症因子IL-1β(白细胞介素-1β)、CXCL-1 (C-X-C基序趋化因子配体1)、MCP-1(单核细胞趋化蛋白-1)以及NF κ B抑制剂IkB、NF- kb磷酸化水平的变化。结果:高氧引起新生大鼠肺损伤,尼达尼布治疗可减轻肺损伤。此外,尼达尼布可减轻高氧诱导的大鼠肺细胞凋亡。我们进一步观察到,尼达尼布可以减轻肺样本中由高氧引起的炎症反应。此外,尼达尼能使BPD大鼠NF-κB通路失活。结论:尼达尼布通过靶向NF-κB信号通路减轻高氧诱导的肺损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nintedanib alleviates hyperoxia-induced lung injury via targeting NF-κB signalling pathway in rat model of bronchopulmonary dysplasia.

Introduction: Bronchopulmonary dysplasia (BPD) is a common chronic respiratory disease in premature infants. Hyperoxia is the main pathogenic factor of BPD. Nintedanib is a small-molecule tyrosine kinase inhibitor that has been confirmed to affect several cellular processes in different diseases. The aim of this study was to explore the function of nintedanib in the treatment of BPD.

Material and methods: We used newborn Sprague-Dawley rats (postnatal day 1) to establish an in vivo BPD model by hyperoxia induction. Nintedanib was intraperitoneally injected into rats. Haematoxylin and eosin (H&E) staining was applied to detect lung injury in BPD rats. Cell apoptosis was determined by TUNEL (TdT-mediated dUTP nick end labelling) assay. Western blotting was applied to detect level changes of inflammatory factors IL-1β (interleukin-1 beta), CXCL-1 (C-X-C motif chemokine ligand 1), MCP-1 (monocyte chemotactic protein-1), as well as the phosphorylation of IkB (NF kappa B inhibitor) and NF-kB (nuclear factor kappa-B) in lung samples.

Results: Hyperoxia resulted in lung injury in neonatal rats, while nintedanib treatment relieved the injury. Furthermore, nintedanib alleviated hyperoxia-induced apoptosis in rat lungs. We further observed that an inflammatory response caused by hyperoxia in lung samples was attenuated by nintedanib administration. Additionally, nintedanib inactivated the NF-κB pathway in BPD rats.

Conclusions: Nintedanib alleviates hyperoxia-induced lung injury via targeting the NF-κB signalling pathway.

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来源期刊
Folia histochemica et cytobiologica
Folia histochemica et cytobiologica 生物-生化与分子生物学
CiteScore
2.80
自引率
6.70%
发文量
56
审稿时长
6-12 weeks
期刊介绍: "Folia Histochemica et Cytobiologica" is an international, English-language journal publishing articles in the areas of histochemistry, cytochemistry and cell & tissue biology. "Folia Histochemica et Cytobiologica" was established in 1963 under the title: ‘Folia Histochemica et Cytochemica’ by the Polish Histochemical and Cytochemical Society as a journal devoted to the rapidly developing fields of histochemistry and cytochemistry. In 1984, the profile of the journal was broadened to accommodate papers dealing with cell and tissue biology, and the title was accordingly changed to "Folia Histochemica et Cytobiologica". "Folia Histochemica et Cytobiologica" is published quarterly, one volume a year, by the Polish Histochemical and Cytochemical Society.
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