训练诱导的血浆miR-29a-3p由骨骼肌分泌，有助于小鼠抵抗运动的代谢适应。

IF 6.6 2区医学 Q1 ENDOCRINOLOGY & METABOLISM

Molecular Metabolism Pub Date : 2025-05-23 DOI:10.1016/j.molmet.2025.102173

Paola Pinto-Hernandez , Manuel Fernandez-Sanjurjo , Daan Paget , Xurde M. Caravia , David Roiz-Valle , Juan Castilla-Silgado , Sergio Diez-Robles , Almudena Coto-Vilcapoma , David Fernandez-Vivero , Pau Gama-Perez , Pablo M. Garcia-Roves , Carlos Lopez-Otin , Juleen R. Zierath , Anna Krook , Benjamin Fernandez-Garcia , Cristina Tomas-Zapico , Eduardo Iglesias-Gutierrez

{"title":"训练诱导的血浆miR-29a-3p由骨骼肌分泌，有助于小鼠抵抗运动的代谢适应。","authors":"Paola Pinto-Hernandez , Manuel Fernandez-Sanjurjo , Daan Paget , Xurde M. Caravia , David Roiz-Valle , Juan Castilla-Silgado , Sergio Diez-Robles , Almudena Coto-Vilcapoma , David Fernandez-Vivero , Pau Gama-Perez , Pablo M. Garcia-Roves , Carlos Lopez-Otin , Juleen R. Zierath , Anna Krook , Benjamin Fernandez-Garcia , Cristina Tomas-Zapico , Eduardo Iglesias-Gutierrez","doi":"10.1016/j.molmet.2025.102173","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>The adaptive response to different models of regular exercise involves complex tissue crosstalk. Our aim was to explore the involvement of extracellular vesicle (EV) microRNAs (miRNAs) in this process, the secretory role of skeletal muscle and its functional metabolic interaction with the liver.</div></div><div><h3>Methods</h3><div>Plasma EV miRNAs obtained from mice after 4-weeks of endurance or resistance training were sequenced. Subsequent experiments using trained genetically modified mouse models and in vitro approaches involving knock-down and electrostimulated cells, were conducted.</div></div><div><h3>Results</h3><div>Resistance training increased the expression of a group of 11 miRNAs functionally divided into two clusters. Among them, miR-29a-3p emerges as a molecular mediator released in EVs by skeletal muscle, with a relevant role in adaptation to endurance training, by contributing to modulate the expression and secretion of other miRNAs associated with training and regulating processes related to substrate availability, transport, and metabolic use in skeletal muscle and liver.</div></div><div><h3>Conclusions</h3><div>Our study suggests that miR-29a-3p is a training-induced molecular mediator in the response and adaptation to resistance training, possibly due to its regulatory role in energy metabolism in skeletal muscle in response to exercise.</div></div>","PeriodicalId":18765,"journal":{"name":"Molecular Metabolism","volume":"98 ","pages":"Article 102173"},"PeriodicalIF":6.6000,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Training-induced plasma miR-29a-3p is secreted by skeletal muscle and contributes to metabolic adaptations to resistance exercise in mice\",\"authors\":\"Paola Pinto-Hernandez , Manuel Fernandez-Sanjurjo , Daan Paget , Xurde M. Caravia , David Roiz-Valle , Juan Castilla-Silgado , Sergio Diez-Robles , Almudena Coto-Vilcapoma , David Fernandez-Vivero , Pau Gama-Perez , Pablo M. Garcia-Roves , Carlos Lopez-Otin , Juleen R. Zierath , Anna Krook , Benjamin Fernandez-Garcia , Cristina Tomas-Zapico , Eduardo Iglesias-Gutierrez\",\"doi\":\"10.1016/j.molmet.2025.102173\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><div>The adaptive response to different models of regular exercise involves complex tissue crosstalk. Our aim was to explore the involvement of extracellular vesicle (EV) microRNAs (miRNAs) in this process, the secretory role of skeletal muscle and its functional metabolic interaction with the liver.</div></div><div><h3>Methods</h3><div>Plasma EV miRNAs obtained from mice after 4-weeks of endurance or resistance training were sequenced. Subsequent experiments using trained genetically modified mouse models and in vitro approaches involving knock-down and electrostimulated cells, were conducted.</div></div><div><h3>Results</h3><div>Resistance training increased the expression of a group of 11 miRNAs functionally divided into two clusters. Among them, miR-29a-3p emerges as a molecular mediator released in EVs by skeletal muscle, with a relevant role in adaptation to endurance training, by contributing to modulate the expression and secretion of other miRNAs associated with training and regulating processes related to substrate availability, transport, and metabolic use in skeletal muscle and liver.</div></div><div><h3>Conclusions</h3><div>Our study suggests that miR-29a-3p is a training-induced molecular mediator in the response and adaptation to resistance training, possibly due to its regulatory role in energy metabolism in skeletal muscle in response to exercise.</div></div>\",\"PeriodicalId\":18765,\"journal\":{\"name\":\"Molecular Metabolism\",\"volume\":\"98 \",\"pages\":\"Article 102173\"},\"PeriodicalIF\":6.6000,\"publicationDate\":\"2025-05-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Metabolism\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212877825000808\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENDOCRINOLOGY & METABOLISM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Metabolism","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212877825000808","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}

引用次数: 0

摘要

目前尚不清楚对不同运动模式的适应性反应是否由骨骼肌释放的细胞外囊泡（EV） microRNAs （miRNAs）及其功能性代谢作用介导。我们对小鼠在4周耐力或阻力训练后获得的携带mirna的血浆ev进行了测序。抗阻运动增加了分为两个功能簇的11-miRNA谱的表达。使用转基因小鼠和细胞模型，我们已经确定miR-29a-3p是ev在响应骨骼肌收缩时释放的分子介质。此外，miR-29a-3p似乎也通过调节肌肉和肝脏中其他mirna的表达和分泌以及能量代谢，在适应抗阻训练中发挥了相关作用。综上所述，我们的研究表明miR-29a-3p可能是一种训练诱导的分子介质，在抵抗训练的反应和适应中发挥作用，可能是由于其在能量代谢中的调节作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Training-induced plasma miR-29a-3p is secreted by skeletal muscle and contributes to metabolic adaptations to resistance exercise in mice

Objective

The adaptive response to different models of regular exercise involves complex tissue crosstalk. Our aim was to explore the involvement of extracellular vesicle (EV) microRNAs (miRNAs) in this process, the secretory role of skeletal muscle and its functional metabolic interaction with the liver.

Methods

Plasma EV miRNAs obtained from mice after 4-weeks of endurance or resistance training were sequenced. Subsequent experiments using trained genetically modified mouse models and in vitro approaches involving knock-down and electrostimulated cells, were conducted.

Results

Resistance training increased the expression of a group of 11 miRNAs functionally divided into two clusters. Among them, miR-29a-3p emerges as a molecular mediator released in EVs by skeletal muscle, with a relevant role in adaptation to endurance training, by contributing to modulate the expression and secretion of other miRNAs associated with training and regulating processes related to substrate availability, transport, and metabolic use in skeletal muscle and liver.

Conclusions

Our study suggests that miR-29a-3p is a training-induced molecular mediator in the response and adaptation to resistance training, possibly due to its regulatory role in energy metabolism in skeletal muscle in response to exercise.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Molecular Metabolism ENDOCRINOLOGY & METABOLISM-

CiteScore

14.50

自引率

2.50%

发文量

219

审稿时长

43 days

期刊介绍： Molecular Metabolism is a leading journal dedicated to sharing groundbreaking discoveries in the field of energy homeostasis and the underlying factors of metabolic disorders. These disorders include obesity, diabetes, cardiovascular disease, and cancer. Our journal focuses on publishing research driven by hypotheses and conducted to the highest standards, aiming to provide a mechanistic understanding of energy homeostasis-related behavior, physiology, and dysfunction. We promote interdisciplinary science, covering a broad range of approaches from molecules to humans throughout the lifespan. Our goal is to contribute to transformative research in metabolism, which has the potential to revolutionize the field. By enabling progress in the prognosis, prevention, and ultimately the cure of metabolic disorders and their long-term complications, our journal seeks to better the future of health and well-being.