Diogo Milani, Vanessa B Bardella, Frederico Hickmann, Alberto S Corrêa, Andrew P Michel, Pablo Mora, José M Rico-Porras, Teresa Palomeque, Pedro Lorite, Diogo C Cabral-de-Mello
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Using comparative genomic hybridization (CGH) with male and female genomic DNAs (gDNA), this study examined a total of 25 Pentatomidae species to uncover repetitive DNA dynamics and their role in chromosome differentiation, especially sex chromosome differentiation. New karyotype data for nine species reinforces the chromosomal stasis in Pentatomidae for macro-chromosomal structure. However, significant variability in repetitive DNA patterns on autosomes and sex chromosomes has been revealed despite the karyotypic conservation. Autosomal signals varied in intensity and distribution, with some species exhibiting terminal enrichment of repeats, while others displayed dispersed patterns. Sex chromosomes showed distinct hybridization patterns, with the Y chromosome exhibiting more significant variability compared to the X. 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引用次数: 0
摘要
性染色体在不同物种中独立进化,表现出独特的进化模式,包括分化、退化和重复的DNA积累。半角蝽科具有高度保守的二倍体数目2n = 14,性染色体系统为XX/XY,即2n = 14,12 a + XY。因此,它代表了一个有趣的群体来研究重复序列在保守核型中的重组,即没有大的染色体重排。本文利用比较基因组杂交技术(CGH)对25个五子虫科物种的雄性和雌性基因组DNA (gDNA)进行了分析,揭示了重复DNA动态及其在染色体分化,特别是性染色体分化中的作用。九种新的核型数据加强了五尾虫科大染色体结构的染色体停滞。然而,尽管核型守恒,在常染色体和性染色体上重复DNA模式的显著变异性已被揭示。常染色体信号的强度和分布各不相同,一些物种表现出末端重复序列的富集,而另一些物种则表现出分散的模式。性染色体表现出明显的杂交模式,Y染色体比x染色体表现出更显著的可变性。这些发现强调了性染色体的动态性质,并建议进一步研究结合基因组测序和细胞遗传学来揭示序列及其进化背后的机制。
Variable organization of repeats and hidden diversity of XY sex chromosomes in Pentatomidae true Bugs (Hemiptera) revealed through comparative genomic hybridization.
Sex chromosomes have independently evolved in various species, displaying unique evolutionary patterns, including differentiation, degeneration, and repetitive DNA accumulation. Pentatomidae hemipterans are characterized by a highly conserved diploid number of 2n = 14 with a XX/XY sex chromosome system, i.e. 2n = 14, 12 A + XY. Thus, it represents an interesting group for investigating the reorganization of repeats in conserved karyotypes, i.e. the absence of large chromosomal rearrangements. Using comparative genomic hybridization (CGH) with male and female genomic DNAs (gDNA), this study examined a total of 25 Pentatomidae species to uncover repetitive DNA dynamics and their role in chromosome differentiation, especially sex chromosome differentiation. New karyotype data for nine species reinforces the chromosomal stasis in Pentatomidae for macro-chromosomal structure. However, significant variability in repetitive DNA patterns on autosomes and sex chromosomes has been revealed despite the karyotypic conservation. Autosomal signals varied in intensity and distribution, with some species exhibiting terminal enrichment of repeats, while others displayed dispersed patterns. Sex chromosomes showed distinct hybridization patterns, with the Y chromosome exhibiting more significant variability compared to the X. These findings emphasize the dynamic nature of sex chromosomes and suggest further studies combining genomic sequencing and cytogenetics to uncover sequences and the mechanisms behind their evolution.
期刊介绍:
Chromosoma publishes research and review articles on the functional organization of the eukaryotic cell nucleus, with a particular emphasis on the structure and dynamics of chromatin and chromosomes; the expression and replication of genomes; genome organization and evolution; the segregation of genomes during meiosis and mitosis; the function and dynamics of subnuclear compartments; the nuclear envelope and nucleocytoplasmic interactions, and more.
The scope of Chromosoma encompasses genetic, biophysical, molecular and cell biological studies.
Average time from receipt of contributions to first decision: 22 days
Publishes research and review articles on the functional organization of the eukaryotic cell nucleus
Topics include structure and dynamics of chromatin and chromosomes; the expression and replication of genomes; genome organization and evolution; the segregation of genomes during meiosis and mitosis and more
Encompasses genetic, biophysical, molecular and cell biological studies.