{"title":"双歧杆菌改善狼疮性肾炎和调节淋巴细胞亚群的异常分化","authors":"Lihua Wu, Zhirong Guan, Xiaoyu Zhang, Yanan Liu, Shuhong Chi, Xiangguo Duan, Chunxia Su","doi":"10.1093/rheumatology/keaf271","DOIUrl":null,"url":null,"abstract":"Objectives The gut microbiota is crucial in the progression of lupus nephritis (LN), and probiotics have emerged as a potential therapeutic approach. This study investigates the role of Bifidobacterium in LN pathogenesis and its effects on immune cell populations. Methods Clinical samples were collected from 61 systemic lupus erythematosus (SLE) patients, and flow cytometry was used to analyze changes in CD4+ T and B cell subsets. ELISA quantified cytokine secretion and autoantibodies in both human and murine models. Additionally, 16S rRNA sequencing identified variations in gut microbiota abundance among patients and helped screen for potential probiotics. Histopathological examination and immunofluorescence staining assessed kidney injury improvements in MRL/lpr mice treated with Bifidobacterium, its total metabolites, and short-chain fatty acids (SCFAs). Results SLE patients showed decreased gut microbiota abundance and diversity, particularly a reduction in Bifidobacterium. Clinical data revealed lower proportions of memory B cells, central memory T cells, Treg cells, and IL-2, alongside increased effector memory T cells, Th1 and Th17 cells, IL-10, and IFN-γ in LN patients. Supplementation with Bifidobacterium, its metabolites, and SCFAs corrected the imbalances in CD4+ T and B cell subsets and cytokine levels, reduced autoantibody levels in mice, and alleviated kidney damage. Conclusions Bifidobacterium is significantly diminished in SLE patients, influencing kidney injury and immune cell balance in LN. Moreover, Bifidobacterium, its total metabolites and SCFAs’ supplementation in MRL/lpr mice notably improved kidney damage and restored immune cell equilibrium in MRL/lpr mice.","PeriodicalId":21255,"journal":{"name":"Rheumatology","volume":"12 1","pages":""},"PeriodicalIF":4.7000,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bifidobacterium ameliorates lupus nephritis and modulates aberrant differentiation of lymphocyte subsets\",\"authors\":\"Lihua Wu, Zhirong Guan, Xiaoyu Zhang, Yanan Liu, Shuhong Chi, Xiangguo Duan, Chunxia Su\",\"doi\":\"10.1093/rheumatology/keaf271\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objectives The gut microbiota is crucial in the progression of lupus nephritis (LN), and probiotics have emerged as a potential therapeutic approach. This study investigates the role of Bifidobacterium in LN pathogenesis and its effects on immune cell populations. Methods Clinical samples were collected from 61 systemic lupus erythematosus (SLE) patients, and flow cytometry was used to analyze changes in CD4+ T and B cell subsets. ELISA quantified cytokine secretion and autoantibodies in both human and murine models. Additionally, 16S rRNA sequencing identified variations in gut microbiota abundance among patients and helped screen for potential probiotics. Histopathological examination and immunofluorescence staining assessed kidney injury improvements in MRL/lpr mice treated with Bifidobacterium, its total metabolites, and short-chain fatty acids (SCFAs). Results SLE patients showed decreased gut microbiota abundance and diversity, particularly a reduction in Bifidobacterium. Clinical data revealed lower proportions of memory B cells, central memory T cells, Treg cells, and IL-2, alongside increased effector memory T cells, Th1 and Th17 cells, IL-10, and IFN-γ in LN patients. Supplementation with Bifidobacterium, its metabolites, and SCFAs corrected the imbalances in CD4+ T and B cell subsets and cytokine levels, reduced autoantibody levels in mice, and alleviated kidney damage. Conclusions Bifidobacterium is significantly diminished in SLE patients, influencing kidney injury and immune cell balance in LN. Moreover, Bifidobacterium, its total metabolites and SCFAs’ supplementation in MRL/lpr mice notably improved kidney damage and restored immune cell equilibrium in MRL/lpr mice.\",\"PeriodicalId\":21255,\"journal\":{\"name\":\"Rheumatology\",\"volume\":\"12 1\",\"pages\":\"\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2025-05-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Rheumatology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1093/rheumatology/keaf271\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"RHEUMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rheumatology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1093/rheumatology/keaf271","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"RHEUMATOLOGY","Score":null,"Total":0}
Bifidobacterium ameliorates lupus nephritis and modulates aberrant differentiation of lymphocyte subsets
Objectives The gut microbiota is crucial in the progression of lupus nephritis (LN), and probiotics have emerged as a potential therapeutic approach. This study investigates the role of Bifidobacterium in LN pathogenesis and its effects on immune cell populations. Methods Clinical samples were collected from 61 systemic lupus erythematosus (SLE) patients, and flow cytometry was used to analyze changes in CD4+ T and B cell subsets. ELISA quantified cytokine secretion and autoantibodies in both human and murine models. Additionally, 16S rRNA sequencing identified variations in gut microbiota abundance among patients and helped screen for potential probiotics. Histopathological examination and immunofluorescence staining assessed kidney injury improvements in MRL/lpr mice treated with Bifidobacterium, its total metabolites, and short-chain fatty acids (SCFAs). Results SLE patients showed decreased gut microbiota abundance and diversity, particularly a reduction in Bifidobacterium. Clinical data revealed lower proportions of memory B cells, central memory T cells, Treg cells, and IL-2, alongside increased effector memory T cells, Th1 and Th17 cells, IL-10, and IFN-γ in LN patients. Supplementation with Bifidobacterium, its metabolites, and SCFAs corrected the imbalances in CD4+ T and B cell subsets and cytokine levels, reduced autoantibody levels in mice, and alleviated kidney damage. Conclusions Bifidobacterium is significantly diminished in SLE patients, influencing kidney injury and immune cell balance in LN. Moreover, Bifidobacterium, its total metabolites and SCFAs’ supplementation in MRL/lpr mice notably improved kidney damage and restored immune cell equilibrium in MRL/lpr mice.
期刊介绍:
Rheumatology strives to support research and discovery by publishing the highest quality original scientific papers with a focus on basic, clinical and translational research. The journal’s subject areas cover a wide range of paediatric and adult rheumatological conditions from an international perspective. It is an official journal of the British Society for Rheumatology, published by Oxford University Press.
Rheumatology publishes original articles, reviews, editorials, guidelines, concise reports, meta-analyses, original case reports, clinical vignettes, letters and matters arising from published material. The journal takes pride in serving the global rheumatology community, with a focus on high societal impact in the form of podcasts, videos and extended social media presence, and utilizing metrics such as Altmetric. Keep up to date by following the journal on Twitter @RheumJnl.