{"title":"壳聚糖纳米颗粒结合两歧双歧杆菌无细胞上清液对结肠腺癌（Caco-2）细胞系结直肠癌相关基因表达的影响","authors":"Rahimeh Maqsoodi, Masoumeh Saberpour, Bita Bakhshi, Fatemeh Fallah","doi":"10.1186/s12876-025-03923-x","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Colorectal cancer (CRC) is emerged as a global problem with high mortality rate; hence, finding of alternative treatment approaches is essential. The purposes of this research are to assess the impact of chitosan nanoparticles conjugated with the cell free supernatant of Bifidobacterium bifidum (CTNP/B.b-sup) on genes associated with CRC signaling pathways.</p><p><strong>Methods: </strong>The novel nano-drug were fabricated via an ionic gelation technique and analyzed using transmission electron microscopy (TEM) and dynamic light scattering (DLS) methods. The release of protein and entrapment efficiency (EE) of CTNP/B.b-sup were assessed using a BCA protein assay. Following an investigation into the toxicity of CTNP/B.b-sup on Caco-2 cells by MTT assay, the expression of genes associated with CRC signaling pathways was evaluated utilizing real-time PCR method.</p><p><strong>Results: </strong>CTNP/B.b-sup exhibited a suitable morphology with a particle size of 453.1 ± 230.8 nm and zeta potential of 9.11 ± 3.6 mV. The protein released was 75.5% at pH ~ 6.8 within 48 h with 83.3% of EE. The viability of Caco-2 cells against CTNP/B.b-sup was 90.3%. The effects of CTNP/B.b-sup on the expression levels of various oncogenes reveal a significant decrease in the expression of β-Catenin, PI3K, TGF-α, Bcl2, TLR4, CEA, and TGF-β oncogenes by 0.96, 0.37, 0.03, 0.41, 0.88, 0.69, and 0.71-fold, respectively. CTNP/B.b-sup induced the most significant reduction in TGF-α oncogene expression, with a decrease of 0.03-fold. Conversely, the strongest induction was observed in the expression of Caspase9 suppressor, with a 73.4-fold increase.</p><p><strong>Conclusion: </strong>In the present study, the CTNP/B.b-sup was demonstrated to possess the capability of modulating genes associated with CRC progression, thereby highlighting its significant pro-apoptotic potential. It can be concluded that CTNP/B.b-sup is a suitable drug delivery system with anticancer properties, which can be regarded as a complementary therapeutic approach for the treatment of CRC.</p>","PeriodicalId":9129,"journal":{"name":"BMC Gastroenterology","volume":"25 1","pages":"394"},"PeriodicalIF":2.5000,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096731/pdf/","citationCount":"0","resultStr":"{\"title\":\"Effect of chitosan nanoparticles conjugated with the cell free supernatant of Bifidobacterium bifidum on the expression of genes related to colorectal cancer in colon adenocarcinoma (Caco-2) cell line.\",\"authors\":\"Rahimeh Maqsoodi, Masoumeh Saberpour, Bita Bakhshi, Fatemeh Fallah\",\"doi\":\"10.1186/s12876-025-03923-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Colorectal cancer (CRC) is emerged as a global problem with high mortality rate; hence, finding of alternative treatment approaches is essential. The purposes of this research are to assess the impact of chitosan nanoparticles conjugated with the cell free supernatant of Bifidobacterium bifidum (CTNP/B.b-sup) on genes associated with CRC signaling pathways.</p><p><strong>Methods: </strong>The novel nano-drug were fabricated via an ionic gelation technique and analyzed using transmission electron microscopy (TEM) and dynamic light scattering (DLS) methods. The release of protein and entrapment efficiency (EE) of CTNP/B.b-sup were assessed using a BCA protein assay. Following an investigation into the toxicity of CTNP/B.b-sup on Caco-2 cells by MTT assay, the expression of genes associated with CRC signaling pathways was evaluated utilizing real-time PCR method.</p><p><strong>Results: </strong>CTNP/B.b-sup exhibited a suitable morphology with a particle size of 453.1 ± 230.8 nm and zeta potential of 9.11 ± 3.6 mV. The protein released was 75.5% at pH ~ 6.8 within 48 h with 83.3% of EE. The viability of Caco-2 cells against CTNP/B.b-sup was 90.3%. The effects of CTNP/B.b-sup on the expression levels of various oncogenes reveal a significant decrease in the expression of β-Catenin, PI3K, TGF-α, Bcl2, TLR4, CEA, and TGF-β oncogenes by 0.96, 0.37, 0.03, 0.41, 0.88, 0.69, and 0.71-fold, respectively. CTNP/B.b-sup induced the most significant reduction in TGF-α oncogene expression, with a decrease of 0.03-fold. Conversely, the strongest induction was observed in the expression of Caspase9 suppressor, with a 73.4-fold increase.</p><p><strong>Conclusion: </strong>In the present study, the CTNP/B.b-sup was demonstrated to possess the capability of modulating genes associated with CRC progression, thereby highlighting its significant pro-apoptotic potential. It can be concluded that CTNP/B.b-sup is a suitable drug delivery system with anticancer properties, which can be regarded as a complementary therapeutic approach for the treatment of CRC.</p>\",\"PeriodicalId\":9129,\"journal\":{\"name\":\"BMC Gastroenterology\",\"volume\":\"25 1\",\"pages\":\"394\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-05-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096731/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BMC Gastroenterology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1186/s12876-025-03923-x\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"GASTROENTEROLOGY & HEPATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Gastroenterology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12876-025-03923-x","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"GASTROENTEROLOGY & HEPATOLOGY","Score":null,"Total":0}
Effect of chitosan nanoparticles conjugated with the cell free supernatant of Bifidobacterium bifidum on the expression of genes related to colorectal cancer in colon adenocarcinoma (Caco-2) cell line.
Background: Colorectal cancer (CRC) is emerged as a global problem with high mortality rate; hence, finding of alternative treatment approaches is essential. The purposes of this research are to assess the impact of chitosan nanoparticles conjugated with the cell free supernatant of Bifidobacterium bifidum (CTNP/B.b-sup) on genes associated with CRC signaling pathways.
Methods: The novel nano-drug were fabricated via an ionic gelation technique and analyzed using transmission electron microscopy (TEM) and dynamic light scattering (DLS) methods. The release of protein and entrapment efficiency (EE) of CTNP/B.b-sup were assessed using a BCA protein assay. Following an investigation into the toxicity of CTNP/B.b-sup on Caco-2 cells by MTT assay, the expression of genes associated with CRC signaling pathways was evaluated utilizing real-time PCR method.
Results: CTNP/B.b-sup exhibited a suitable morphology with a particle size of 453.1 ± 230.8 nm and zeta potential of 9.11 ± 3.6 mV. The protein released was 75.5% at pH ~ 6.8 within 48 h with 83.3% of EE. The viability of Caco-2 cells against CTNP/B.b-sup was 90.3%. The effects of CTNP/B.b-sup on the expression levels of various oncogenes reveal a significant decrease in the expression of β-Catenin, PI3K, TGF-α, Bcl2, TLR4, CEA, and TGF-β oncogenes by 0.96, 0.37, 0.03, 0.41, 0.88, 0.69, and 0.71-fold, respectively. CTNP/B.b-sup induced the most significant reduction in TGF-α oncogene expression, with a decrease of 0.03-fold. Conversely, the strongest induction was observed in the expression of Caspase9 suppressor, with a 73.4-fold increase.
Conclusion: In the present study, the CTNP/B.b-sup was demonstrated to possess the capability of modulating genes associated with CRC progression, thereby highlighting its significant pro-apoptotic potential. It can be concluded that CTNP/B.b-sup is a suitable drug delivery system with anticancer properties, which can be regarded as a complementary therapeutic approach for the treatment of CRC.
期刊介绍：
BMC Gastroenterology is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of gastrointestinal and hepatobiliary disorders, as well as related molecular genetics, pathophysiology, and epidemiology.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
