白菊精华通过抗炎和皮肤屏障修复机制对特应性皮炎的缓解作用。

IF 3.7 2区 生物学 Q3 CELL BIOLOGY
Molecular and Cellular Biochemistry Pub Date : 2025-09-01 Epub Date: 2025-05-20 DOI:10.1007/s11010-025-05270-7
Congcong Zhu, Junchao Wu, Ya Chen, Tianyou Ma, Huijun Pan, Chuntao Zhai, Zongguang Tai, Zhongjian Chen, Quangang Zhu
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引用次数: 0

摘要

白菊精华(BJE)是一种由药用植物提取物组成的生物活性制剂,因其治疗潜力而被用于护肤品中。特应性皮炎(AD)是一种以表皮屏障功能障碍和免疫失调为特征的慢性炎症性皮肤病。本研究旨在评估BJE的抗炎和皮肤保护作用及其治疗AD的潜在机制。在HaCaT细胞中评估BJE恢复表皮屏障的能力。在lps诱导的RAW264.7细胞中,通过测定NO、IL-6、PGE2和TNF-α来评估BJE的抗炎潜能。Western blot分析评估MAPK通路的调控作用。采用MC903建立AD样小鼠体内模型,记录其体重、耳厚和AD症状。组织学分析量化肥大细胞浸润,western blot检测FLG、LOR和ELOVL6的表达。ELISA法检测TNF-α、IgE、IL-4、IL-13水平。流式细胞术评估BJE对Th细胞表型的影响。BJE显著提高了HaCaT细胞中皮肤屏障蛋白(CERS2、LOR、HAS-1、HAS-2、FLG)的表达。显著降低lps处理RAW264.7的NO、IL-6、PGE2和TNF-α水平,显示其抗炎潜力。从机制上讲,BJE抑制了MAPK的激活。BJE降低了AD样小鼠的耳厚,改善了皮肤病变,缓解了AD症状。此外,BJE有效抑制肥大细胞浸润和角化过度。BJE还降低了TNF-α、IgE、IL-4和IL-13的水平,同时增加了LOR、ELOVL6和FLG的表达。此外,BJE调节Th1, Th2和Th17细胞比例。BJE在HaCaT中促进表皮屏障修复,在RAW264.7中抑制lps诱导的炎症,在ad样小鼠中增强皮肤屏障完整性,并通过恢复Th细胞平衡发挥免疫调节作用。这些发现强调了BJE通过其抗炎和皮肤屏障修复的双重作用治疗AD的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The alleviating effect of Bai-Ju essence on atopic dermatitis through anti-inflammatory and skin barrier repair mechanisms.

Bai-Ju essence (BJE) is a bioactive formulation composed of medicinal plant extracts, utilized in skincare products for its therapeutic potential. Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by epidermal barrier dysfunction and immune dysregulation. This study aimed to evaluate BJE anti-inflammatory and skin-protective effects, and its potential mechanisms in treating AD. The ability of BJE to restore the epidermal barrier was assessed in HaCaT cells. In LPS-induced RAW264.7 cells, the anti-inflammatory potential of BJE was evaluated by measuring NO, IL-6, PGE2, and TNF-α. Western blot analysis was used to assess the regulation of the MAPK pathway. An in vivo AD-like mouse model was established using MC903, and measurements of body weight, ear thickness, and AD symptoms were recorded. Histological analysis quantified mast cell infiltration, while western blot determined FLG, LOR, and ELOVL6 expression. ELISA was used to measure TNF-α, IgE, IL-4, and IL-13 levels. Flow cytometry assessed the effect of BJE on Th cell phenotypes. BJE significantly enhanced skin barrier protein expression (CERS2, LOR, HAS-1, HAS-2, FLG) in HaCaT cells. It significantly reduced the levels of NO, IL-6, PGE2, and TNF-α in LPS-treated RAW264.7, demonstrating its anti-inflammatory potential. Mechanistically, BJE inhibited MAPK activation. BJE decreased ear thickness, improved skin lesions, and relieved AD symptoms in AD-like mice. In addition, BJE effectively suppressed mast cell infiltration and hyperkeratosis. BJE also decreased levels of TNF-α, IgE, IL-4, and IL-13 while increasing LOR, ELOVL6, and FLG expressions. Furthermore, BJE modulated Th1, Th2, and Th17 cell proportions. BJE promoted epidermal barrier repair in HaCaT, suppressed the LPS-induced inflammation in RAW264.7, enhanced the skin barrier integrity in AD-like mice, and exhibited immunomodulatory effects by restoring Th cell balance. These findings highlighted the therapeutic potential of BJE in AD through its dual action of anti-inflammation and skin barrier restoration.

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来源期刊
Molecular and Cellular Biochemistry
Molecular and Cellular Biochemistry 生物-细胞生物学
CiteScore
8.30
自引率
2.30%
发文量
293
审稿时长
1.7 months
期刊介绍: Molecular and Cellular Biochemistry: An International Journal for Chemical Biology in Health and Disease publishes original research papers and short communications in all areas of the biochemical sciences, emphasizing novel findings relevant to the biochemical basis of cellular function and disease processes, as well as the mechanics of action of hormones and chemical agents. Coverage includes membrane transport, receptor mechanism, immune response, secretory processes, and cytoskeletal function, as well as biochemical structure-function relationships in the cell. In addition to the reports of original research, the journal publishes state of the art reviews. Specific subjects covered by Molecular and Cellular Biochemistry include cellular metabolism, cellular pathophysiology, enzymology, ion transport, lipid biochemistry, membrane biochemistry, molecular biology, nuclear structure and function, and protein chemistry.
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