小鼠骨髓小生境细胞的分离及单细胞测序。

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-05-22 DOI:10.1007/7651_2025_630

Yaphet Bustos, Adrienne M Dorrance, Chinmayee Goda

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引用次数: 0

摘要

骨髓（BM）生态位由不同类型的细胞组成，每种细胞在造血和疾病中发挥着不同的作用。单细胞RNA测序使异质组织内的各种细胞群的调查。在本章中，我们描述了通过荧光激活细胞分选单细胞RNA测序从小鼠股骨和胫骨中分离BM小生境细胞的方法。该方法可确保分离出高活力、高纯度的高质量BM小生境细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Isolation of Mouse Bone Marrow Niche Cells for Single-Cell Sequencing.

The bone marrow (BM) niche comprises of diverse cell types, each playing a distinct role in hematopoiesis and disease. Single-cell RNA sequencing enables investigation of various cell populations within heterogeneous tissues. In this chapter, we describe the method to isolate BM niche cells from femurs and tibias of mice by fluorescence-activated cell sorting for single-cell RNA sequencing. This method ensures isolation of high-quality BM niche cells with high viability and purity.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.