中国绵羊和山羊内罗比羊病病毒感染的流行病学、病毒学和病原学研究。

IF 3.8 2区 医学 Q2 VIROLOGY
Journal of Virology Pub Date : 2025-06-17 Epub Date: 2025-05-21 DOI:10.1128/jvi.00006-25
Xin-Yan Yao, Meng-Hang Wang, Xue-Lian Zhang, Xu Zhang, De-Xin Liang, Shao-Han Li, Chun-Yang Lian, Zhi-Hang Lv, Chang-You Xia, Ming-Fa Yang, Jian-Wei Shao, Xin Yin
{"title":"中国绵羊和山羊内罗比羊病病毒感染的流行病学、病毒学和病原学研究。","authors":"Xin-Yan Yao, Meng-Hang Wang, Xue-Lian Zhang, Xu Zhang, De-Xin Liang, Shao-Han Li, Chun-Yang Lian, Zhi-Hang Lv, Chang-You Xia, Ming-Fa Yang, Jian-Wei Shao, Xin Yin","doi":"10.1128/jvi.00006-25","DOIUrl":null,"url":null,"abstract":"<p><p>Nairobi sheep disease virus (NSDV), a tick-borne pathogen in the <i>Orthonairovirus</i> genus of the <i>Nairoviridae</i> family, causes Nairobi sheep disease (NSD), which has mortality rates over 90% in sheep and goats. Historically, NSD outbreaks in East Africa and South Asia have led to considerable economic losses. Although NSDV genomic fragments have been detected in ticks in China, there has been a notable absence of reported infections in local sheep and goats. In this study, we report the first identification of NSDV infection in sheep and goats in China. Through meta-transcriptomics sequencing, NSDV was found in infected spleen tissue with high viral abundance. Epidemiological investigations indicated a 4.1% positive rate for NSDV RNA and a 29.9% seroprevalence rate, confirming the presence of NSDV infection in these herds. Sequence analyses showed that the identified NSDV clustered with those previously found in ticks in China but were distinct from Africa or India strains. Furthermore, these viruses could be categorized into different sub-clades, suggesting the genetic diversity of NSDV in China. Remarkably, NSDV was isolated from infected sheep for the first time in China, and the isolated virus could cause severe clinical signs, leading to sheep mortality. In summary, this study represents the initial identification of NSDV in vertebrate hosts in China and highlights the importance of NSDV surveillance to prevent outbreaks of NSD within the country.IMPORTANCEAs a significant tick-borne virus, Nairobi sheep disease virus (NSDV) has caused devastating disease known as Nairobi sheep disease (NSD) in small ruminants in East Africa and South Asia. Although NSDV genomic fragments have been detected in ticks within China, there has been little information on its infection in local sheep or goats. This study confirms, for the first time, the infection of NSDV among these animals in China. Additionally, the successful isolation of NSDV from infected sheep highlights its capacity to replicate in various cell lines, including those of human origin, thereby underscoring its broad host range and potential for cross-species transmission. Notably, animal experiments demonstrated that the isolated virus strain could cause severe clinical signs in sheep. These findings provide the first evidence for the presence of NSDV infection among sheep and goats in China, underscoring the critical need for NSDV surveillance to prevent outbreaks of NSD within the country.</p>","PeriodicalId":17583,"journal":{"name":"Journal of Virology","volume":" ","pages":"e0000625"},"PeriodicalIF":3.8000,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12172417/pdf/","citationCount":"0","resultStr":"{\"title\":\"Epidemiological, virological, and pathogenic insights into Nairobi sheep disease virus infection in sheep and goats in China.\",\"authors\":\"Xin-Yan Yao, Meng-Hang Wang, Xue-Lian Zhang, Xu Zhang, De-Xin Liang, Shao-Han Li, Chun-Yang Lian, Zhi-Hang Lv, Chang-You Xia, Ming-Fa Yang, Jian-Wei Shao, Xin Yin\",\"doi\":\"10.1128/jvi.00006-25\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Nairobi sheep disease virus (NSDV), a tick-borne pathogen in the <i>Orthonairovirus</i> genus of the <i>Nairoviridae</i> family, causes Nairobi sheep disease (NSD), which has mortality rates over 90% in sheep and goats. Historically, NSD outbreaks in East Africa and South Asia have led to considerable economic losses. Although NSDV genomic fragments have been detected in ticks in China, there has been a notable absence of reported infections in local sheep and goats. In this study, we report the first identification of NSDV infection in sheep and goats in China. Through meta-transcriptomics sequencing, NSDV was found in infected spleen tissue with high viral abundance. Epidemiological investigations indicated a 4.1% positive rate for NSDV RNA and a 29.9% seroprevalence rate, confirming the presence of NSDV infection in these herds. Sequence analyses showed that the identified NSDV clustered with those previously found in ticks in China but were distinct from Africa or India strains. Furthermore, these viruses could be categorized into different sub-clades, suggesting the genetic diversity of NSDV in China. Remarkably, NSDV was isolated from infected sheep for the first time in China, and the isolated virus could cause severe clinical signs, leading to sheep mortality. In summary, this study represents the initial identification of NSDV in vertebrate hosts in China and highlights the importance of NSDV surveillance to prevent outbreaks of NSD within the country.IMPORTANCEAs a significant tick-borne virus, Nairobi sheep disease virus (NSDV) has caused devastating disease known as Nairobi sheep disease (NSD) in small ruminants in East Africa and South Asia. Although NSDV genomic fragments have been detected in ticks within China, there has been little information on its infection in local sheep or goats. This study confirms, for the first time, the infection of NSDV among these animals in China. Additionally, the successful isolation of NSDV from infected sheep highlights its capacity to replicate in various cell lines, including those of human origin, thereby underscoring its broad host range and potential for cross-species transmission. Notably, animal experiments demonstrated that the isolated virus strain could cause severe clinical signs in sheep. These findings provide the first evidence for the presence of NSDV infection among sheep and goats in China, underscoring the critical need for NSDV surveillance to prevent outbreaks of NSD within the country.</p>\",\"PeriodicalId\":17583,\"journal\":{\"name\":\"Journal of Virology\",\"volume\":\" \",\"pages\":\"e0000625\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2025-06-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12172417/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Virology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1128/jvi.00006-25\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/5/21 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"VIROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Virology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1128/jvi.00006-25","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/21 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"VIROLOGY","Score":null,"Total":0}
引用次数: 0

摘要

内罗毕绵羊病病毒(NSDV)是内罗毕病毒科正形空气病毒属的一种蜱传病原体,可引起内罗毕绵羊病(NSD),绵羊和山羊的死亡率超过90%。从历史上看,非传染性疾病在东非和南亚的暴发造成了相当大的经济损失。虽然在中国的蜱虫中检测到NSDV基因组片段,但在当地绵羊和山羊中没有明显的感染报告。在这项研究中,我们报告了中国首次在绵羊和山羊中发现NSDV感染。通过meta转录组测序,发现NSDV在感染脾组织中具有高病毒丰度。流行病学调查显示,猪群NSDV RNA阳性率为4.1%,血清阳性率为29.9%,证实猪群存在NSDV感染。序列分析表明,鉴定的NSDV与以前在中国蜱中发现的NSDV聚集在一起,但与非洲或印度的毒株不同。此外,这些病毒可以被划分为不同的亚进化支,这表明中国NSDV的遗传多样性。值得注意的是,这是中国首次从感染绵羊中分离出NSDV,分离出的病毒可引起严重的临床症状,导致绵羊死亡。总之,本研究代表了中国脊椎动物宿主中NSDV的初步鉴定,并强调了NSDV监测对防止NSDV在国内暴发的重要性。作为一种重要的蜱传病毒,内罗毕羊病病毒(NSDV)在东非和南亚的小反刍动物中引起了被称为内罗毕羊病(NSD)的毁灭性疾病。虽然在中国境内的蜱中检测到NSDV基因组片段,但在当地绵羊或山羊中感染的信息很少。本研究首次证实了中国这些动物中NSDV的感染。此外,从受感染的绵羊中成功分离出NSDV,突出了其在各种细胞系(包括人类起源的细胞系)中复制的能力,从而强调了其广泛的宿主范围和跨物种传播的潜力。值得注意的是,动物实验表明,分离的病毒株可在绵羊中引起严重的临床症状。这些发现为中国绵羊和山羊中存在非sdv感染提供了第一个证据,强调了监测非sdv以防止国内爆发非sdv的迫切需要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Epidemiological, virological, and pathogenic insights into Nairobi sheep disease virus infection in sheep and goats in China.

Nairobi sheep disease virus (NSDV), a tick-borne pathogen in the Orthonairovirus genus of the Nairoviridae family, causes Nairobi sheep disease (NSD), which has mortality rates over 90% in sheep and goats. Historically, NSD outbreaks in East Africa and South Asia have led to considerable economic losses. Although NSDV genomic fragments have been detected in ticks in China, there has been a notable absence of reported infections in local sheep and goats. In this study, we report the first identification of NSDV infection in sheep and goats in China. Through meta-transcriptomics sequencing, NSDV was found in infected spleen tissue with high viral abundance. Epidemiological investigations indicated a 4.1% positive rate for NSDV RNA and a 29.9% seroprevalence rate, confirming the presence of NSDV infection in these herds. Sequence analyses showed that the identified NSDV clustered with those previously found in ticks in China but were distinct from Africa or India strains. Furthermore, these viruses could be categorized into different sub-clades, suggesting the genetic diversity of NSDV in China. Remarkably, NSDV was isolated from infected sheep for the first time in China, and the isolated virus could cause severe clinical signs, leading to sheep mortality. In summary, this study represents the initial identification of NSDV in vertebrate hosts in China and highlights the importance of NSDV surveillance to prevent outbreaks of NSD within the country.IMPORTANCEAs a significant tick-borne virus, Nairobi sheep disease virus (NSDV) has caused devastating disease known as Nairobi sheep disease (NSD) in small ruminants in East Africa and South Asia. Although NSDV genomic fragments have been detected in ticks within China, there has been little information on its infection in local sheep or goats. This study confirms, for the first time, the infection of NSDV among these animals in China. Additionally, the successful isolation of NSDV from infected sheep highlights its capacity to replicate in various cell lines, including those of human origin, thereby underscoring its broad host range and potential for cross-species transmission. Notably, animal experiments demonstrated that the isolated virus strain could cause severe clinical signs in sheep. These findings provide the first evidence for the presence of NSDV infection among sheep and goats in China, underscoring the critical need for NSDV surveillance to prevent outbreaks of NSD within the country.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Virology
Journal of Virology 医学-病毒学
CiteScore
10.10
自引率
7.40%
发文量
906
审稿时长
1 months
期刊介绍: Journal of Virology (JVI) explores the nature of the viruses of animals, archaea, bacteria, fungi, plants, and protozoa. We welcome papers on virion structure and assembly, viral genome replication and regulation of gene expression, genetic diversity and evolution, virus-cell interactions, cellular responses to infection, transformation and oncogenesis, gene delivery, viral pathogenesis and immunity, and vaccines and antiviral agents.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信