Circ 0020938通过miR-142-5p/DSG4轴抑制绒山羊毛囊干细胞增殖。

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jiamian Du, Menghua Sui, Zhihao Song, Shuangshuang Liang, Yujie Zheng, Xin Wang
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引用次数: 0

摘要

背景:陕北白绒山羊是优良的绒山羊品种,其市场青睐从次毛囊开始的羊绒。毛囊在出生前后成熟,每个毛囊反复经历一个生长周期,包括三个不同的阶段:生长期、衰退期和休止期。了解毛囊循环变化的分子机制对优化毛囊功能和提高羊绒产量具有重要意义。方法:构建毛囊周期中circRNA表达谱,鉴定差异表达的circRNA,重点关注在毛囊生长期间高表达的circ 0020938。通过功能实验评估circ 0020938对毛囊干细胞(HFSCs)增殖的影响。构建竞争内源性RNA (ceRNA)网络来研究circ 0020938、miR-142-5p和DSG4之间的相互作用。通过拯救实验验证circ 0020938对HFSCs增殖和DSG4表达的影响。结果:我们发现circ 0020938抑制了HFSCs的增殖。进一步分析发现circ 0020938作为miR-142-5p的海绵,减轻了DSG4的抑制。此外,我们证实了DSG4抑制HFSCs的增殖,这表明它在毛囊周期中调节增殖和分化之间的平衡中起关键作用。营救实验显示circ 0020938对HFSCs增殖的抑制被miR-142-5p部分逆转。结论:我们的研究为circRNA在毛囊周期中对HFSCs增殖的调控作用提供了新的见解。结果表明circ 0020938作为miRNA海绵,通过miR-142-5p/DSG4轴抑制HFSCs的增殖,从而促进毛囊周期的正常进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Circ 0020938 inhibits hair follicle stem cells proliferation via the miR-142-5p/DSG4 axis in cashmere goats.

Background: Shaanbei white cashmere goat is an excellent cashmere goat breed, and its market favored cashmere from the secondary hair follicles. Hair follicles mature around birth and each hair follicle repeatedly undergoes a growth cycle that comprises three distinct stages: anagen, catagen and telogen. Understanding the molecular mechanisms controlling cyclic hair follicle changes is essential for optimizing hair follicle function and improving cashmere production.

Methods: The circRNA expression profile in the hair follicle cycle was constructed and differentially expressed circRNAs were identified, with particular focus on circ 0020938, which was highly expressed during anagen. The functional assays were performed to assess the effect of circ 0020938 on hair follicle stem cells (HFSCs) proliferation. Competing endogenous RNA (ceRNA) network was constructed to investigate the interaction between circ 0020938, miR-142-5p, and DSG4. Rescue experiment was conducted to validate the impact of circ 0020938 on HFSCs proliferation and DSG4 expression.

Results: We found that circ 0020938 inhibited HFSCs proliferation. Further analysis revealed that circ 0020938 acted as a sponge for miR-142-5p, alleviating the repression of DSG4. Additionally, we confirmed that DSG4 inhibited HFSCs proliferation, suggesting that it play a key role in regulating the balance between proliferation and differentiation during the hair follicle cycle. Rescue experiments showed that the inhibition of HFSCs proliferation by circ 0020938 was partially reversed by miR-142-5p.

Conclusion: Our study provides novel insights into the regulatory role of circRNA in HFSCs proliferation during the hair follicle cycle. The results demonstrate that circ 0020938 acts as a miRNA sponge and inhibits HFSCs proliferation through the miR-142-5p/DSG4 axis, thereby contributing to the proper progression of the hair follicle cycle.

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来源期刊
BMC Genomics
BMC Genomics 生物-生物工程与应用微生物
CiteScore
7.40
自引率
4.50%
发文量
769
审稿时长
6.4 months
期刊介绍: BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.
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