人巨噬细胞系统:活性和功能形态。

Bibliotheca anatomica Pub Date : 1988-01-01
H Michna
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引用次数: 0

摘要

使用一种新开发的、并不特别困难的方法,可以从结缔组织中大量提取人类巨噬细胞。利用光镜、扫描电镜和透射电镜等方法将这些巨噬细胞与小鼠腹膜巨噬细胞进行比较。细胞悬浮液的无菌性和巨噬细胞的高产量使得组织细胞的首次体外研究得以进行,这与经典的“微渗出包被表面法”形成了对比。人类巨噬细胞与小鼠腹腔巨噬细胞的活性已经通过多种组织化学和免疫学技术进行了评估。这些方法证明了在极端剧烈体育锻炼的典型条件下,健康人和小鼠的巨噬细胞活性的调节,与早期单独在动物身上的实验结果一致。骨骼肌系统在这些实验条件下发生的退行性变化表明免疫系统细胞的入侵,这可以解释巨噬细胞活性增加的原因。这些结果在一个新的理论概念中找到了自己的位置,支持巨噬细胞和其他免疫系统细胞在骨骼肌系统病理变性和再生过程中的合作的普遍有效性。研究表明,人类和小鼠巨噬细胞的活动增加是由极端剧烈的体育锻炼引起的,只要人们能够将它们命令到一个渐进的压力发生方案中,这非常符合“警报反应”阶段的概念。巨噬细胞的活性被证明对间充质起源肿瘤的介质、吞噬的初始阶段、进一步的生化恶化、细胞毒性和细胞数量敏感;然而,在长期的实验中,这并不能阻止肉瘤的快速生长。巨噬细胞在携带肉瘤的动物中迁移减弱的证据引起了人们对那些能够积极调节迁移活动的物质的兴趣。一方面,在短期实验中,巨噬细胞在带肉瘤动物体内的迁移性能通过引入合成代谢类固醇激素而提高。然而,另一方面,在短期和长期实验研究中,巨噬细胞活性的进一步参数取得了不同程度的成功。(摘要删节为400字)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The human macrophage system: activity and functional morphology.

Macrophages of humans could be extracted in large numbers from the connective tissue using a newly developed, not particularly difficult method. These macrophages were compared with the peritoneal macrophages of mice using light-, scanning and transmission electron-microscopic methods. The sterility of the cell suspension and the high yield of macrophages has allowed the first in vitro study of histiocytes to take place, in contrast to the classic 'microexudate-coated surface method'. The activity of the human in comparison with peritoneal murine macrophages has been evaluated using numerous histochemical and immunological techniques. These methods prove a modulation of the macrophage activity of healthy humans and mice under exemplary conditions of extremely strenuous physical exercising, in accordance with earlier experimental findings on animals alone. The degenerative changes which occur under these experimental conditions in the skeletal muscular system show an invasion of cells of the immune system, which are integrated into an explanation of the increased activity of macrophages. These results find their place in a new theoretical concept supporting the general validity of the co-operation of macrophages and other cells of the immune system in pathological degeneration and regeneration processes in the skeletal muscular system. It has been shown that the increased activity of human and murine macrophages brought about by extreme strenuous physical exercising, insofar as one is able to order them into a progressive scheme of stress happenings, fit very well into the concepts of the 'alarm reaction' phase. The activity of macrophages proves to be sensitive to the mediators of tumours of mesenchymal origin, with respect to the initial stage of phagocytosis, to the further biochemical deterioration, to the cytotoxicity and to the amount of cells; this, however, is not able to halt the rapid growth of sarcoma in a long term experiment. The proof of a weakened migration of macrophages in sarcoma-bearing animals raises the interest in those substances which are able to positively modulate the migration activity. On the one hand the migratory performance of macrophages in sarcoma-bearing animals in a short-term experiment was increased by the introduction of an anabolic steroid hormone. On the other hand, however, a different degree of success was registered for the further parameters of macrophage activity during short- and long-term experimental investigations.(ABSTRACT TRUNCATED AT 400 WORDS)

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