阿魏酸通过抑制电压依赖性Ca2+通道抑制豚鼠回肠纵向平滑肌收缩

IF 3 3区医学 Q2 PHARMACOLOGY & PHARMACY

Journal of pharmacological sciences Pub Date : 2025-05-14 DOI:10.1016/j.jphs.2025.05.012

Keisuke Obara , Kento Yoshioka , Aya Shimada, Sakika Ichihara, Wakaba Kinami, Futaba Makino, Naho Takazakura, Miwa Enomoto, Yoshio Tanaka

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引用次数: 0

摘要

研究阿魏酸（FA）对豚鼠回肠纵向平滑肌（ILSM）收缩的影响。FA （3 × 10−4-3 × 10−3 M）对乙酰胆碱、组胺、前列腺素F2α和血清素诱导的ILSM收缩具有浓度依赖性、可逆性和非竞争性，pD′2值为~ 3。FA也具有浓度依赖性和可逆性地抑制kcl诱导的ILSM收缩。FA（10−3 M）抑制ILSM收缩约50%，也降低了维拉帕米敏感的，kcl诱导的A7r5细胞内Ca2+增加约35%。这些结果表明，FA抑制ILSM收缩，主要是通过电压依赖性Ca2+通道可逆抑制Ca2+内流。

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Ferulic acid suppresses guinea pig ileal longitudinal smooth muscle contractions by inhibiting voltage-dependent Ca2+ channels

We investigated the effects of ferulic acid (FA) on contractions in guinea pig ileal longitudinal smooth muscle (ILSM). FA (3 × 10⁻⁴–3 × 10⁻³ M) inhibited ILSM contractions induced by acetylcholine, histamine, prostaglandin F_2α, and serotonin concentration-dependently, reversibly, and noncompetitively with pD′₂ values of ∼3. FA also concentration-dependently and reversibly inhibited KCl-induced ILSM contractions. FA (10⁻³ M), which inhibited ILSM contractions by ∼50 %, also reduced verapamil-sensitive, KCl-induced intracellular Ca²⁺ increases in A7r5 cells by ∼35 %. These results suggest that FA inhibits ILSM contraction, primarily through the reversible inhibition of Ca²⁺ influx via voltage-dependent Ca²⁺ channels.

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来源期刊

Journal of pharmacological sciences 医学-药学

CiteScore

6.20

自引率

2.90%

发文量

104

审稿时长

31 days

期刊介绍： Journal of Pharmacological Sciences (JPS) is an international open access journal intended for the advancement of pharmacological sciences in the world. The Journal welcomes submissions in all fields of experimental and clinical pharmacology, including neuroscience, and biochemical, cellular, and molecular pharmacology for publication as Reviews, Full Papers or Short Communications. Short Communications are short research article intended to provide novel and exciting pharmacological findings. Manuscripts concerning descriptive case reports, pharmacokinetic and pharmacodynamic studies without pharmacological mechanism and dose-response determinations are not acceptable and will be rejected without peer review. The ethnopharmacological studies are also out of the scope of this journal. Furthermore, JPS does not publish work on the actions of biological extracts unknown chemical composition.