Insights from a Long-Term Outdoor Mesocosm Study: eDNA Metabarcoding Reveals Exacerbated but Transient Impacts from a Nanoenabled Pesticide Formulation (Nano-TiO2-Coated Carbendazim) on Freshwater Microbial Communities.

Fungicides currently encompass the second-most-used class of agricultural pesticides globally. Residues are frequently detected in freshwater, leading to undesired ecological impacts. Nanoenabled pesticide formulations have recently gained prominence in the scientific literature and have been suggested to exhibit favorable properties over conventional pesticide formulations by facilitating reductions in emissions toward nontarget locations. However, data on unintended effects on nontarget aquatic communities are scarce, especially concerning microbial communities. In this study, long-term effects of nano titanium-dioxide- (nTiO₂)-coated carbendazim and its constituents on (pelagic) freshwater microbial communities in simulated agricultural ditches were investigated over a period of 14 weeks using environmental DNA (eDNA) metabarcoding. Impacts on bacterial diversity (α and β) were observed 2 weeks after the treatment application and most pronounced in the nTiO₂-coated carbendazim treatment, followed by its active substance, i.e., noncoated carbendazim. The observed patterns possibly imply that nTiO₂-coated carbendazim imposed more pronounced and potentially delayed or extended effects compared to the noncoated form of carbendazim. Bacterial communities also proved to be resilient under the tested conditions as they returned to the control-state within 5 weeks after the treatment application. Overall, our data suggest that eDNA metabarcoding data on microbial communities can help uncover time-dependent effects of nanoformulated pesticides.