小鼠慢性酒精消耗通过细胞器特异性乙酰化重编程肝脏代谢。

IF 6.1 2区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS
Mirjavid Aghayev, Megan R McMullen, Serguei Ilchenko, Andrea Arias-Alvarado, Victor Lufi, Jack Mathis, Hannah Marchuk, Tsung-Heng Tsai, Guo-Fang Zhang, Laura E Nagy, Takhar Kasumov
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引用次数: 0

摘要

翻译后乙酰辅酶a对蛋白质的乙酰化作用是蛋白质稳态和底物代谢的重要调节因子。肝脏中的乙醇代谢诱导蛋白质积累、乙酰化和代谢中断。虽然乙酰化影响酶的活性和稳定性,但其在乙醇相关蛋白质积累和代谢功能障碍中的作用尚不清楚。通过稳定的基于同位素的蛋白质组学、乙酰组学和慢性乙醇肝损伤小鼠模型的代谢分析,我们证明了乙醇诱导肝脏脂肪变性、炎症、氧化应激和与蛋白质转换改变相关的蛋白质病变。乙醇增加了与氧化应激和解毒相关的胞质蛋白周转,同时减少了线粒体代谢酶的周转。它还提高了线粒体酶和核组蛋白的乙酰化,细胞质变化很小,损害了线粒体蛋白的降解。这些变化与酰基辅酶a和酰基肉碱、氨基酸和三羧酸(TCA)循环中间体水平的改变有关,反映了脂肪酸氧化、氮处理和TCA循环活性的受损。这些结果表明,乙醇诱导的乙酰化有助于肝损伤,靶向乙酰化可能为酒精诱导的肝脏疾病提供治疗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chronic alcohol consumption reprograms hepatic metabolism through organelle-specific acetylation in mice.

Post-translational acetylation of proteins by acetyl-CoA is a crucial regulator of proteostasis and substrate metabolism. Ethanol metabolism in the liver induces protein accumulation, acetylation and metabolic disruption. While acetylation impacts enzyme activity and stability, its role in ethanol-related protein accumulation and metabolic dysfunction remains unclear. Using stable isotope-based proteomics, acetylomics, and metabolic profiling in a mouse model of chronic ethanol-induced liver injury, we demonstrate that ethanol induces hepatic steatosis, inflammation, oxidative stress, and proteinopathy linked to altered protein turnover. Ethanol increased the cytosolic protein turnover related to oxidative stress and detoxification, while reducing turnover of mitochondrial metabolic enzymes. It also elevated the acetylation of mitochondrial enzymes and nuclear histones with minimal cytosolic changes, impairing mitochondrial protein degradation. These changes were associated with altered levels of acyl-CoAs and acyl-carnitines, amino acids, and tricarboxylic acid (TCA) cycle intermediates, reflecting impaired fatty acid oxidation, nitrogen disposal and TCA cycle activities. These results suggest that ethanol-induced acetylation contributes to liver injury and that targeting acetylation may offer treatment for alcohol-induced liver diseases.

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来源期刊
Molecular & Cellular Proteomics
Molecular & Cellular Proteomics 生物-生化研究方法
CiteScore
11.50
自引率
4.30%
发文量
131
审稿时长
84 days
期刊介绍: The mission of MCP is to foster the development and applications of proteomics in both basic and translational research. MCP will publish manuscripts that report significant new biological or clinical discoveries underpinned by proteomic observations across all kingdoms of life. Manuscripts must define the biological roles played by the proteins investigated or their mechanisms of action. The journal also emphasizes articles that describe innovative new computational methods and technological advancements that will enable future discoveries. Manuscripts describing such approaches do not have to include a solution to a biological problem, but must demonstrate that the technology works as described, is reproducible and is appropriate to uncover yet unknown protein/proteome function or properties using relevant model systems or publicly available data. Scope: -Fundamental studies in biology, including integrative "omics" studies, that provide mechanistic insights -Novel experimental and computational technologies -Proteogenomic data integration and analysis that enable greater understanding of physiology and disease processes -Pathway and network analyses of signaling that focus on the roles of post-translational modifications -Studies of proteome dynamics and quality controls, and their roles in disease -Studies of evolutionary processes effecting proteome dynamics, quality and regulation -Chemical proteomics, including mechanisms of drug action -Proteomics of the immune system and antigen presentation/recognition -Microbiome proteomics, host-microbe and host-pathogen interactions, and their roles in health and disease -Clinical and translational studies of human diseases -Metabolomics to understand functional connections between genes, proteins and phenotypes
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