基于RT-RPA和CRISPR-Cas12a的呼吸道合胞病毒检测。

IF 2.8 4区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Experimental Biology and Medicine Pub Date : 2025-05-01 eCollection Date: 2025-01-01 DOI:10.3389/ebm.2025.10387

Ariya Khamwut, Juthamas Nimnual, Nantinee Chomta, Pattaraporn Nimsamer, Oraphan Mayuramart, Pornchai Kaewsapsak, Siripat Pasittungkul, Yong Poovorawan, Sunchai Payungporn

{"title":"基于RT-RPA和CRISPR-Cas12a的呼吸道合胞病毒检测。","authors":"Ariya Khamwut, Juthamas Nimnual, Nantinee Chomta, Pattaraporn Nimsamer, Oraphan Mayuramart, Pornchai Kaewsapsak, Siripat Pasittungkul, Yong Poovorawan, Sunchai Payungporn","doi":"10.3389/ebm.2025.10387","DOIUrl":null,"url":null,"abstract":"Human respiratory syncytial virus (hRSV) is one of the most prevalent viruses infecting children globally. In this study, we employed the RT-RPA with CRISPR/Cas12a detection methodology to detect and differentiate RSV-A and RSV-B, particularly in resource-limited settings. The detection limit for RSV-A and RSV-B was approximately 102 and 103 copies/reaction, respectively. The assay revealed 100% specificity in detecting both RSV-A and RSV-B. Diagnostic accuracy was 90.32 and 93.55% for RSV-A and RSV-B, respectively, compared to RT-qPCR. These data indicate a proficient strategy for RSV screening, demonstrating promise for prospective applications in detecting diverse viral infections.","PeriodicalId":12163,"journal":{"name":"Experimental Biology and Medicine","volume":"250 ","pages":"10387"},"PeriodicalIF":2.8000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078183/pdf/","citationCount":"0","resultStr":"{\"title\":\"Detection of respiratory syncytial virus based on RT-RPA and CRISPR-Cas12a.\",\"authors\":\"Ariya Khamwut, Juthamas Nimnual, Nantinee Chomta, Pattaraporn Nimsamer, Oraphan Mayuramart, Pornchai Kaewsapsak, Siripat Pasittungkul, Yong Poovorawan, Sunchai Payungporn\",\"doi\":\"10.3389/ebm.2025.10387\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Human respiratory syncytial virus (hRSV) is one of the most prevalent viruses infecting children globally. In this study, we employed the RT-RPA with CRISPR/Cas12a detection methodology to detect and differentiate RSV-A and RSV-B, particularly in resource-limited settings. The detection limit for RSV-A and RSV-B was approximately 102 and 103 copies/reaction, respectively. The assay revealed 100% specificity in detecting both RSV-A and RSV-B. Diagnostic accuracy was 90.32 and 93.55% for RSV-A and RSV-B, respectively, compared to RT-qPCR. These data indicate a proficient strategy for RSV screening, demonstrating promise for prospective applications in detecting diverse viral infections.\",\"PeriodicalId\":12163,\"journal\":{\"name\":\"Experimental Biology and Medicine\",\"volume\":\"250 \",\"pages\":\"10387\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2025-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078183/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental Biology and Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3389/ebm.2025.10387\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental Biology and Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3389/ebm.2025.10387","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}

引用次数: 0

摘要

人呼吸道合胞病毒（hRSV）是全球最流行的儿童感染病毒之一。在本研究中，我们采用RT-RPA结合CRISPR/Cas12a检测方法来检测和区分RSV-A和RSV-B，特别是在资源有限的环境中。RSV-A和RSV-B的检出限分别约为102和103拷贝/反应。该方法检测RSV-A和RSV-B的特异性均为100%。与RT-qPCR相比，RSV-A和RSV-B的诊断准确率分别为90.32%和93.55%。这些数据表明，RSV筛选的策略是熟练的，显示了在检测各种病毒感染方面的潜在应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Detection of respiratory syncytial virus based on RT-RPA and CRISPR-Cas12a.

Human respiratory syncytial virus (hRSV) is one of the most prevalent viruses infecting children globally. In this study, we employed the RT-RPA with CRISPR/Cas12a detection methodology to detect and differentiate RSV-A and RSV-B, particularly in resource-limited settings. The detection limit for RSV-A and RSV-B was approximately 10² and 10³ copies/reaction, respectively. The assay revealed 100% specificity in detecting both RSV-A and RSV-B. Diagnostic accuracy was 90.32 and 93.55% for RSV-A and RSV-B, respectively, compared to RT-qPCR. These data indicate a proficient strategy for RSV screening, demonstrating promise for prospective applications in detecting diverse viral infections.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

6.00

自引率

0.00%

发文量

157

审稿时长

1 months

期刊介绍： Experimental Biology and Medicine (EBM) is a global, peer-reviewed journal dedicated to the publication of multidisciplinary and interdisciplinary research in the biomedical sciences. EBM provides both research and review articles as well as meeting symposia and brief communications. Articles in EBM represent cutting edge research at the overlapping junctions of the biological, physical and engineering sciences that impact upon the health and welfare of the world''s population. Topics covered in EBM include: Anatomy/Pathology; Biochemistry and Molecular Biology; Bioimaging; Biomedical Engineering; Bionanoscience; Cell and Developmental Biology; Endocrinology and Nutrition; Environmental Health/Biomarkers/Precision Medicine; Genomics, Proteomics, and Bioinformatics; Immunology/Microbiology/Virology; Mechanisms of Aging; Neuroscience; Pharmacology and Toxicology; Physiology; Stem Cell Biology; Structural Biology; Systems Biology and Microphysiological Systems; and Translational Research.