Zheying Zhang, Yifei Han, Yang Yang, Xianglong Li, Yifan Han, Shuai Zhang, Yan Zou, Yu Zhang, Yitian Xie, Ying Sun, Jiateng Zhong, Baoshun Du, Shenglei Li, Na Li
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引用次数: 0
摘要
目的：阐明长链非编码RNA VPS9D1-AS1影响结直肠癌血管生成的机制。Western blot和qRT-PCR检测VPS9D1-AS1在结直肠癌中的表达。采用细胞生物学、体外成管实验和绒毛膜尿囊膜血管实验检测VPS9D1-AS1对人脐静脉内皮细胞（HUVECs） VEGFA的调控作用及对HUVECs增殖、迁移和侵袭的影响。通过染色质免疫沉淀（ChIP）和双荧光素酶测定来验证转录因子与VPS9D1-AS1启动子区结合的具体位点。VPS9D1-AS1在结直肠癌中高表达。干扰VPS9D1-AS1可抑制HUVECs的增殖、侵袭和迁移。机制上，VPS9D1-AS1通过上调VEGFA表达，激活下游PI3K/AKT通路促进血管生成。此外，CEBPB是数据库预测的VPS9D1-AS1的转录因子，ChIP实验结果表明，CEBPB可以直接结合到VPS9D1-AS1启动子区-698 bp ~ -794 bp位点。双荧光素酶实验结果表明，CEBPB能增强VPS9D1-AS1启动子活性，促进其转录。VPS9D1-AS1可被CEBPB转录因子激活，并靶向VEGFA激活其下游通路促进结直肠癌血管生成，这可能表明VPS9D1-AS1在调节结直肠癌血管生成中起关键作用。
Long noncoding RNA VPS9D1-AS1 promotes angiogenesis in colorectal cancer by regulating the VEGFA signalling pathway.
To clarify the mechanism of long non-coding RNA VPS9D1-AS1 affecting angiogenesis in colorectal cancer (CRC). Western blot and qRT-PCR assays were performed to detect the expression of VPS9D1-AS1 in colorectal cancer. The effects of VPS9D1-AS1 regulating VEGFA and affecting the proliferation, migration and invasion of human umbilical vein endothelial cells (HUVECs) were examined using cell biology, in vitro tubeformation and Chorioallantoic membrane vascular assay. Chromatin Immunoprecipitation (ChIP) and dual luciferase assays were performed to verify the specific sites of transcription factor binding to the promoter region of VPS9D1-AS1. VPS9D1-AS1 is highly expressed in colorectal cancer. Interfering with VPS9D1-AS1 inhibited the proliferation, invasion and migration of HUVECs. Mechanistically, VPS9D1-AS1 can promote angiogenesis by upregulating VEGFA expression and activating the downstream PI3K/AKT pathway. In addition, CEBPB is a transcription factor of VPS9D1-AS1 predicted by database, and the results of ChIP experiments showed that CEBPB could directly bind to the VPS9D1-AS1 promoter region at the -698 bp to -794 bp site. The results of dual luciferase assay showed that CEBPB could enhance VPS9D1-AS1 promoter activity and promote its transcription. VPS9D1-AS1 can be activated by CEBPB transcription factor and target VEGFA to activate its downstream pathway to promote colorectal cancer angiogenesis, which may suggest that VPS9D1-AS1 is critical for regulating colorectal cancer angiogenesis.
期刊介绍：
The American Journal of Cancer Research (AJCR) (ISSN 2156-6976), is an independent open access, online only journal to facilitate rapid dissemination of novel discoveries in basic science and treatment of cancer. It was founded by a group of scientists for cancer research and clinical academic oncologists from around the world, who are devoted to the promotion and advancement of our understanding of the cancer and its treatment. The scope of AJCR is intended to encompass that of multi-disciplinary researchers from any scientific discipline where the primary focus of the research is to increase and integrate knowledge about etiology and molecular mechanisms of carcinogenesis with the ultimate aim of advancing the cure and prevention of this increasingly devastating disease. To achieve these aims AJCR will publish review articles, original articles and new techniques in cancer research and therapy. It will also publish hypothesis, case reports and letter to the editor. Unlike most other open access online journals, AJCR will keep most of the traditional features of paper print that we are all familiar with, such as continuous volume, issue numbers, as well as continuous page numbers to retain our comfortable familiarity towards an academic journal.
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