电刺激辅助CS/凝胶/MWCNTs导电支架在皮肤组织工程中的应用

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology and Bioengineering Pub Date : 2025-05-15 DOI:10.1002/bit.29025

Zixian Liu,Zijun Ma,Nini Li,Jinyao Zhang,Meng Li,Lu Han,Rong Cheng,Zhizhong Shen,Dan Han,Shengbo Sang

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引用次数: 0

摘要

水凝胶支架具有良好的力学性能和生物相容性，在组织工程中具有很大的应用潜力。然而，固有的导电性不足限制了其在需要电刺激的领域的应用。为了解决这一问题，用不同浓度的多壁碳纳米管（MWCNTs）制备了壳聚糖(CS)/明胶（Gel）支架。结果表明，MWCNT的掺入显著提高了支架的导电性和机械强度，与其他配方相比，CS/Gel/0.3% MWCNT支架具有更好的生物相容性。此外，植入支架的成纤维细胞对电刺激反应积极，显示增殖增加，I型和III型胶原蛋白表达升高。这些发现突出了CS/Gel/MWCNTs支架在皮肤组织工程中促进伤口愈合的潜力。

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CS/Gel/MWCNTs Conductive Scaffolds Assisted by Electrical Stimulus for Skin Tissue Engineering.

Hydrogel scaffolds show high potential in tissue engineering due to excellent mechanical properties and biocompatibility. However, an inherent lack of conductivity limits its application in areas requiring electrical stimulation. To address this issue, chitosan (CS)/gelatin (Gel) scaffolds were prepared with various concentrations of multi-walled carbon nanotubes (MWCNTs). Results indicated that MWCNT incorporation significantly improved both the electrical conductivity and mechanical strength of the scaffolds, with the CS/Gel/0.3% MWCNTs scaffold demonstrating superior biocompatibility compared to other formulations. Additionally, fibroblasts seeded onto the scaffolds responded positively to electrical stimulation, showing increased proliferation and elevated expression of type I and type III collagen. These findings highlight the potential of CS/Gel/MWCNTs scaffolds to enhance wound healing in skin tissue engineering.

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来源期刊

Biotechnology and Bioengineering 工程技术-生物工程与应用微生物

CiteScore

7.90

自引率

5.30%

发文量

280

审稿时长

2.1 months

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