宫颈癌患者红细胞中人乳头瘤病毒DNA的检测。

IF 5.7 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Obstetrics and gynecology Pub Date : 2025-05-15 DOI:10.1097/aog.0000000000005932

Sue Li,Emily Oatman,Janos Tanyi,Sarah H Kim,Lori Cory,Nilam S Mangalmurti

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引用次数: 0

摘要

红细胞（rbc）具有结合和包庇病毒DNA的潜力，为检测人乳头瘤病毒（HPV）提供了一种新的方法。用荧光标记的HPV CpG培养的红细胞以浓度依赖的方式获得HPV DNA。用定量聚合酶链反应（PCR）检测HPV阳性宫颈癌细胞（CaSki细胞系）培养的红细胞获得HPV 16 DNA。与这些结果一致的是，通过定量PCR在5例宫颈癌或不典型增生患者的红细胞上检测到HPV 16 DNA，但没有在健康对照红细胞上检测到。检测宫颈癌患者红细胞上的HPV 16 DNA强调了红细胞结合DNA作为未来基于血液的HPV筛查的底物的潜力。

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Detection of Human Papillomavirus DNA on Red Blood Cells in Patients With Cervical Cancer.

Red blood cells (RBCs) have the potential to bind and harbor viral DNA, providing a novel approach to detecting human papillomavirus (HPV). Red blood cells incubated with fluorescently labeled HPV CpG acquired HPV DNA in a concentration-dependent manner. Red blood cells incubated with HPV-positive cervical cancer cells (CaSki cell line) acquired HPV 16 DNA detected by quantitative polymerase chain reaction (PCR). Consistent with these results, HPV 16 DNA was detected by quantitative PCR on RBCs from five patients with cervical cancer or dysplasia but not on healthy control RBCs. Detection of HPV 16 DNA on RBCs from patients with cervical cancer underscores the potential of RBC-bound DNA as a substrate for future blood-based HPV screening.

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来源期刊

Obstetrics and gynecology 医学-妇产科学

CiteScore

11.10

自引率

4.20%

发文量

867

审稿时长

1 months

期刊介绍： "Obstetrics & Gynecology," affectionately known as "The Green Journal," is the official publication of the American College of Obstetricians and Gynecologists (ACOG). Since its inception in 1953, the journal has been dedicated to advancing the clinical practice of obstetrics and gynecology, as well as related fields. The journal's mission is to promote excellence in these areas by publishing a diverse range of articles that cover translational and clinical topics. "Obstetrics & Gynecology" provides a platform for the dissemination of evidence-based research, clinical guidelines, and expert opinions that are essential for the continuous improvement of women's health care. The journal's content is designed to inform and educate obstetricians, gynecologists, and other healthcare professionals, ensuring that they stay abreast of the latest developments and best practices in their field.