The effect of the daidzein on anastomosis healing in a colitis model.

Background: In this experimental colitis model, it is aimed to evaluate effects of enterally administered daidzein on colonic anastomoses of rats.

Methods: 48 male Wistar albino rats, 8-10 weeks old, were used. They were randomly divided into 8 groups, containing 6 rats each. Half of the randomly selected were given water rectally using a catheter and the other half received 2 cc of 4% acetic acid solution by the same way to cause colitis. Five days after the procedure, the rats were operated on and 4 cm long colonic segment, 6 cm proximal to the anal verge, was resected and an end-to-end colonic anastomosis was performed for each. Daidzein was administered orally to predetermined groups. Relaparatomies were performed on the 3rd or 7th postoperative days and the anastomotic colonic segments were resected.

Results: According to the statistical comparison of the parameters obtained by biochemical, histopathological and physiological measurements of the anastomotic tissue; there was no positive effect of enteral daidzein administration on anastomotic healing in terms of hydroxyproline level and anastomotic burst pressure in groups with or without colitis in the early and late postoperative period. In addition, daidzein was not effective in reducing oxidative stress markers such as antioxidant enzymes, glutathione peroxidase and superoxide dismutase. In the late postoperative period, beneficial effect of daidzein administration was observed on the pathology damage score only in the groups without colitis.

Conclusion: No positive effects of daidzein on healing of colon anastomosis were observed in both colitis and colitis-free environments.