{"title":"双能x线吸收仪检测蛛网膜下腔出血小鼠模型神经源性肺水肿。","authors":"Tatsushi Mutoh, Hiroaki Aono, Yushi Mutoh, Tatsuya Ishikawa","doi":"10.1002/ame2.70019","DOIUrl":null,"url":null,"abstract":"<p>Murine subarachnoid hemorrhage (SAH) induced using the filament perforation method is a useful in vivo experimental model to investigate the pathophysiological mechanisms in the brain underlying SAH. However, identifying mice with comorbid acute neurogenic pulmonary edema (NPE), a life-threatening systemic consequence often induced by SAH, in this model is difficult without histopathological investigations. Herein, we present an imaging procedure involving dual-energy X-ray absorptiometry (DXA) to identify NPE in a murine model of SAH. We quantified the lung lean mass (LM) and compared the relationship between micro-computed tomography (CT) evidence of Hounsfield unit (HU) values and histopathological findings of PE. Of the 85 mice with successful induction of SAH by filament perforation, 16 (19%) had NPE, as verified by postmortem histology. The DXA-LM values correlate well with CT-HU levels (<i>r</i> = 0.63, <i>p</i> < 0.0001). Regarding the relationship between LM and HU in mice with post-SAH NPE, the LM was positively associated with HU values (<i>r</i><sup>2</sup> = 0.43; <i>p</i> = 0.0056). A receiver operating characteristics curve of LM revealed a sensitivity of 87% and specificity of 57% for detecting PE, with a similar area under the curve as the HU (0.79 ± 0.06 vs. 0.84 ± 0.07; <i>p</i> = 0.21). These data suggest that confirming acute NPE using DXA-LM is a valuable method for selecting a clinically relevant murine NPE model that could be used in future experimental SAH studies.</p>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":"8 6","pages":"1146-1151"},"PeriodicalIF":0.0000,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ame2.70019","citationCount":"0","resultStr":"{\"title\":\"Dual-energy X-ray absorptiometry for detecting neurogenic pulmonary edema in a mouse model of subarachnoid hemorrhage\",\"authors\":\"Tatsushi Mutoh, Hiroaki Aono, Yushi Mutoh, Tatsuya Ishikawa\",\"doi\":\"10.1002/ame2.70019\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Murine subarachnoid hemorrhage (SAH) induced using the filament perforation method is a useful in vivo experimental model to investigate the pathophysiological mechanisms in the brain underlying SAH. However, identifying mice with comorbid acute neurogenic pulmonary edema (NPE), a life-threatening systemic consequence often induced by SAH, in this model is difficult without histopathological investigations. Herein, we present an imaging procedure involving dual-energy X-ray absorptiometry (DXA) to identify NPE in a murine model of SAH. We quantified the lung lean mass (LM) and compared the relationship between micro-computed tomography (CT) evidence of Hounsfield unit (HU) values and histopathological findings of PE. Of the 85 mice with successful induction of SAH by filament perforation, 16 (19%) had NPE, as verified by postmortem histology. The DXA-LM values correlate well with CT-HU levels (<i>r</i> = 0.63, <i>p</i> < 0.0001). Regarding the relationship between LM and HU in mice with post-SAH NPE, the LM was positively associated with HU values (<i>r</i><sup>2</sup> = 0.43; <i>p</i> = 0.0056). A receiver operating characteristics curve of LM revealed a sensitivity of 87% and specificity of 57% for detecting PE, with a similar area under the curve as the HU (0.79 ± 0.06 vs. 0.84 ± 0.07; <i>p</i> = 0.21). These data suggest that confirming acute NPE using DXA-LM is a valuable method for selecting a clinically relevant murine NPE model that could be used in future experimental SAH studies.</p>\",\"PeriodicalId\":93869,\"journal\":{\"name\":\"Animal models and experimental medicine\",\"volume\":\"8 6\",\"pages\":\"1146-1151\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-04-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ame2.70019\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal models and experimental medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/ame2.70019\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Health Professions\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal models and experimental medicine","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/ame2.70019","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Health Professions","Score":null,"Total":0}
Dual-energy X-ray absorptiometry for detecting neurogenic pulmonary edema in a mouse model of subarachnoid hemorrhage
Murine subarachnoid hemorrhage (SAH) induced using the filament perforation method is a useful in vivo experimental model to investigate the pathophysiological mechanisms in the brain underlying SAH. However, identifying mice with comorbid acute neurogenic pulmonary edema (NPE), a life-threatening systemic consequence often induced by SAH, in this model is difficult without histopathological investigations. Herein, we present an imaging procedure involving dual-energy X-ray absorptiometry (DXA) to identify NPE in a murine model of SAH. We quantified the lung lean mass (LM) and compared the relationship between micro-computed tomography (CT) evidence of Hounsfield unit (HU) values and histopathological findings of PE. Of the 85 mice with successful induction of SAH by filament perforation, 16 (19%) had NPE, as verified by postmortem histology. The DXA-LM values correlate well with CT-HU levels (r = 0.63, p < 0.0001). Regarding the relationship between LM and HU in mice with post-SAH NPE, the LM was positively associated with HU values (r2 = 0.43; p = 0.0056). A receiver operating characteristics curve of LM revealed a sensitivity of 87% and specificity of 57% for detecting PE, with a similar area under the curve as the HU (0.79 ± 0.06 vs. 0.84 ± 0.07; p = 0.21). These data suggest that confirming acute NPE using DXA-LM is a valuable method for selecting a clinically relevant murine NPE model that could be used in future experimental SAH studies.