Erik Neiva Ribeiro de Carvalho Reis, Germano de Lelis Bezerra Júnior, Leonardo de Freitas Silva, Tárik Ocon Braga Polo, Rayane Aquino de Amorim, Daniela Ponzoni, Roberta Okamoto, Rafael Scaf de Molon, Leonardo Perez Faverani, Idelmo Rangel Garcia Junior
{"title":"牙釉质基质衍生物与骨移植替代物联合应用对大鼠颅骨骨再生的组织学、组织计量学和免疫组织化学研究。","authors":"Erik Neiva Ribeiro de Carvalho Reis, Germano de Lelis Bezerra Júnior, Leonardo de Freitas Silva, Tárik Ocon Braga Polo, Rayane Aquino de Amorim, Daniela Ponzoni, Roberta Okamoto, Rafael Scaf de Molon, Leonardo Perez Faverani, Idelmo Rangel Garcia Junior","doi":"10.1007/s10006-025-01390-y","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to evaluate the bone repair process of standardized calvaria defects in rats treated with different bone substitute materials.</p><p><strong>Methods: </strong>Critical size bone defects with 7 mm diameter were created in the right parietal bone of forty-eight rats using a trephine burr. The animals were randomly allocated to four different experimental groups according to the materials used to fill the defect, as follow: (1) Autogenous group: defects were filled with autogenous bone; (2) Bio-Oss group: defects were filled with demineralized bovine bone mineral; (3) EMD group: defects were filled with Emdogain<sup>®</sup>; and (4) EMD + Bone group: defects were filled with a combination of both Bone Ceramic<sup>®</sup> and Emdogain<sup>®</sup>. Euthanasia was performed after 30 and 60 days.</p><p><strong>Results: </strong>Histometric analysis demonstrated for the 30-day period that all groups presented areas of bone neoformation, with the EMD + Bone group showing increased percentage of bone neoformation. For the 60-day period, there was a trend towards greater bone neoformation compared to the 30-day period, with a statistically significant difference between the EMD + Bone vs. Bio-Oss groups (p = 0.036). The percentage of new bone formation was significantly higher in the autogenous group compared to all the others. Moderate immunostaining of osteocalcin was observed in all groups at 30 days, which increased significantly in the 60-day period.</p><p><strong>Conclusion: </strong>This study demonstrated suitable biological properties in the association of Emdogain<sup>®</sup> with Bone Ceramic<sup>®</sup>. However, further laboratory studies are needed to evaluate the osteoinductive properties of these materials, as well as their behavior in humans through clinical studies.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":47251,"journal":{"name":"Oral and Maxillofacial Surgery-Heidelberg","volume":"29 1","pages":"95"},"PeriodicalIF":1.7000,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of bone regeneration in rat calvaria using combination of enamel matrix derivate and bone graft substitute: a histological, histometric and immunohistochemical study.\",\"authors\":\"Erik Neiva Ribeiro de Carvalho Reis, Germano de Lelis Bezerra Júnior, Leonardo de Freitas Silva, Tárik Ocon Braga Polo, Rayane Aquino de Amorim, Daniela Ponzoni, Roberta Okamoto, Rafael Scaf de Molon, Leonardo Perez Faverani, Idelmo Rangel Garcia Junior\",\"doi\":\"10.1007/s10006-025-01390-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>This study aimed to evaluate the bone repair process of standardized calvaria defects in rats treated with different bone substitute materials.</p><p><strong>Methods: </strong>Critical size bone defects with 7 mm diameter were created in the right parietal bone of forty-eight rats using a trephine burr. The animals were randomly allocated to four different experimental groups according to the materials used to fill the defect, as follow: (1) Autogenous group: defects were filled with autogenous bone; (2) Bio-Oss group: defects were filled with demineralized bovine bone mineral; (3) EMD group: defects were filled with Emdogain<sup>®</sup>; and (4) EMD + Bone group: defects were filled with a combination of both Bone Ceramic<sup>®</sup> and Emdogain<sup>®</sup>. Euthanasia was performed after 30 and 60 days.</p><p><strong>Results: </strong>Histometric analysis demonstrated for the 30-day period that all groups presented areas of bone neoformation, with the EMD + Bone group showing increased percentage of bone neoformation. For the 60-day period, there was a trend towards greater bone neoformation compared to the 30-day period, with a statistically significant difference between the EMD + Bone vs. Bio-Oss groups (p = 0.036). The percentage of new bone formation was significantly higher in the autogenous group compared to all the others. Moderate immunostaining of osteocalcin was observed in all groups at 30 days, which increased significantly in the 60-day period.</p><p><strong>Conclusion: </strong>This study demonstrated suitable biological properties in the association of Emdogain<sup>®</sup> with Bone Ceramic<sup>®</sup>. However, further laboratory studies are needed to evaluate the osteoinductive properties of these materials, as well as their behavior in humans through clinical studies.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>\",\"PeriodicalId\":47251,\"journal\":{\"name\":\"Oral and Maxillofacial Surgery-Heidelberg\",\"volume\":\"29 1\",\"pages\":\"95\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-05-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Oral and Maxillofacial Surgery-Heidelberg\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1007/s10006-025-01390-y\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Oral and Maxillofacial Surgery-Heidelberg","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s10006-025-01390-y","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Evaluation of bone regeneration in rat calvaria using combination of enamel matrix derivate and bone graft substitute: a histological, histometric and immunohistochemical study.
Purpose: This study aimed to evaluate the bone repair process of standardized calvaria defects in rats treated with different bone substitute materials.
Methods: Critical size bone defects with 7 mm diameter were created in the right parietal bone of forty-eight rats using a trephine burr. The animals were randomly allocated to four different experimental groups according to the materials used to fill the defect, as follow: (1) Autogenous group: defects were filled with autogenous bone; (2) Bio-Oss group: defects were filled with demineralized bovine bone mineral; (3) EMD group: defects were filled with Emdogain®; and (4) EMD + Bone group: defects were filled with a combination of both Bone Ceramic® and Emdogain®. Euthanasia was performed after 30 and 60 days.
Results: Histometric analysis demonstrated for the 30-day period that all groups presented areas of bone neoformation, with the EMD + Bone group showing increased percentage of bone neoformation. For the 60-day period, there was a trend towards greater bone neoformation compared to the 30-day period, with a statistically significant difference between the EMD + Bone vs. Bio-Oss groups (p = 0.036). The percentage of new bone formation was significantly higher in the autogenous group compared to all the others. Moderate immunostaining of osteocalcin was observed in all groups at 30 days, which increased significantly in the 60-day period.
Conclusion: This study demonstrated suitable biological properties in the association of Emdogain® with Bone Ceramic®. However, further laboratory studies are needed to evaluate the osteoinductive properties of these materials, as well as their behavior in humans through clinical studies.
期刊介绍：
Oral & Maxillofacial Surgery founded as Mund-, Kiefer- und Gesichtschirurgie is a peer-reviewed online journal. It is designed for clinicians as well as researchers.The quarterly journal offers comprehensive coverage of new techniques, important developments and innovative ideas in oral and maxillofacial surgery and interdisciplinary aspects of cranial, facial and oral diseases and their management. The journal publishes papers of the highest scientific merit and widest possible scope on work in oral and maxillofacial surgery as well as supporting specialties. Practice-oriented articles help improve the methods used in oral and maxillofacial surgery.Every aspect of oral and maxillofacial surgery is fully covered through a range of invited review articles, clinical and research articles, technical notes, abstracts, and case reports. Specific topics are: aesthetic facial surgery, clinical pathology, computer-assisted surgery, congenital and craniofacial deformities, dentoalveolar surgery, head and neck oncology, implant dentistry, oral medicine, orthognathic surgery, reconstructive surgery, skull base surgery, TMJ and trauma.Time-limited reviewing and electronic processing allow to publish articles as fast as possible. Accepted articles are rapidly accessible online.Clinical studies submitted for publication have to include a declaration that they have been approved by an ethical committee according to the World Medical Association Declaration of Helsinki 1964 (last amendment during the 52nd World Medical Association General Assembly, Edinburgh, Scotland, October 2000). Experimental animal studies have to be carried out according to the principles of laboratory animal care (NIH publication No 86-23, revised 1985).
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
