Piercarlo Minoretti, Enzo Emanuele, Celia García-Chico, Kayvan Khoramipour, Alejandro Santos-Lozano, Eugenia V Di Brizzi, Simone Lista
{"title":"死海和磁化盐水对牛皮癣生物标志物自噬介导的调节:一项体外研究","authors":"Piercarlo Minoretti, Enzo Emanuele, Celia García-Chico, Kayvan Khoramipour, Alejandro Santos-Lozano, Eugenia V Di Brizzi, Simone Lista","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Dysregulated autophagy is linked to abnormal keratinocyte differentiation and persistent psoriatic inflammation. Smart fluids, such as Dead Sea Water (DSW) and saline magnetized water (MW), have emerged as potential non-pharmacological autophagy activators. This study evaluates their effects on psoriasis-like keratinocytes, focusing on calcitonin gene-related peptide (CGRP), a neuropeptide involved in pruritus and inflammation, and secreted frizzled-related protein 4 (SFRP4), whose reduced expression contributes to epidermal hyperplasia. The role of autophagy in mediating these effects was also investigated.</p><p><strong>Methods: </strong>Polycytokine-stimulated HaCaT keratinocytes were treated with DSW or saline MW. CGRP and SFRP4 expression levels were assessed alongside autophagy markers beclin-1 and LC3B. The involvement of autophagy was confirmed using wortmannin, an autophagy inhibitor.</p><p><strong>Results: </strong>Both DSW (4.7 ± 1.9 a.u.) and saline MW (3.6 ± 1.6 a.u.) significantly reduced CGRP expression compared to controls (non-magnetized saline: 7.5 ± 2.3 a.u.; distilled water: 7.6 ± 2.5 a.u.; all p< 0.001). While both fluids enhanced SFRP4 expression equally (p = 0.78), saline MW showed superior CGRP inhibition (p< 0.001). Both fluids mitigated polycytokine-induced reductions in beclin-1 and LC3B levels (all p< 0.001), with saline MW showing more pronounced effects (p< 0.05). Wortmannin impaired the effects of both fluids on CGRP and SFRP4, indicating autophagy mediation.</p><p><strong>Conclusions: </strong>DSW and saline MW show promise as sustainable active ingredients for topical formulations targeting psoriatic inflammation via autophagy activation.</p>","PeriodicalId":94154,"journal":{"name":"Neuro endocrinology letters","volume":"46 1","pages":"27-32"},"PeriodicalIF":0.0000,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Autophagy-mediated regulation of psoriasis biomarkers by Dead Sea and magnetized saline waters: An in vitro study.\",\"authors\":\"Piercarlo Minoretti, Enzo Emanuele, Celia García-Chico, Kayvan Khoramipour, Alejandro Santos-Lozano, Eugenia V Di Brizzi, Simone Lista\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Dysregulated autophagy is linked to abnormal keratinocyte differentiation and persistent psoriatic inflammation. Smart fluids, such as Dead Sea Water (DSW) and saline magnetized water (MW), have emerged as potential non-pharmacological autophagy activators. This study evaluates their effects on psoriasis-like keratinocytes, focusing on calcitonin gene-related peptide (CGRP), a neuropeptide involved in pruritus and inflammation, and secreted frizzled-related protein 4 (SFRP4), whose reduced expression contributes to epidermal hyperplasia. The role of autophagy in mediating these effects was also investigated.</p><p><strong>Methods: </strong>Polycytokine-stimulated HaCaT keratinocytes were treated with DSW or saline MW. CGRP and SFRP4 expression levels were assessed alongside autophagy markers beclin-1 and LC3B. The involvement of autophagy was confirmed using wortmannin, an autophagy inhibitor.</p><p><strong>Results: </strong>Both DSW (4.7 ± 1.9 a.u.) and saline MW (3.6 ± 1.6 a.u.) significantly reduced CGRP expression compared to controls (non-magnetized saline: 7.5 ± 2.3 a.u.; distilled water: 7.6 ± 2.5 a.u.; all p< 0.001). While both fluids enhanced SFRP4 expression equally (p = 0.78), saline MW showed superior CGRP inhibition (p< 0.001). Both fluids mitigated polycytokine-induced reductions in beclin-1 and LC3B levels (all p< 0.001), with saline MW showing more pronounced effects (p< 0.05). Wortmannin impaired the effects of both fluids on CGRP and SFRP4, indicating autophagy mediation.</p><p><strong>Conclusions: </strong>DSW and saline MW show promise as sustainable active ingredients for topical formulations targeting psoriatic inflammation via autophagy activation.</p>\",\"PeriodicalId\":94154,\"journal\":{\"name\":\"Neuro endocrinology letters\",\"volume\":\"46 1\",\"pages\":\"27-32\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-04-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Neuro endocrinology letters\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neuro endocrinology letters","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Autophagy-mediated regulation of psoriasis biomarkers by Dead Sea and magnetized saline waters: An in vitro study.
Background: Dysregulated autophagy is linked to abnormal keratinocyte differentiation and persistent psoriatic inflammation. Smart fluids, such as Dead Sea Water (DSW) and saline magnetized water (MW), have emerged as potential non-pharmacological autophagy activators. This study evaluates their effects on psoriasis-like keratinocytes, focusing on calcitonin gene-related peptide (CGRP), a neuropeptide involved in pruritus and inflammation, and secreted frizzled-related protein 4 (SFRP4), whose reduced expression contributes to epidermal hyperplasia. The role of autophagy in mediating these effects was also investigated.
Methods: Polycytokine-stimulated HaCaT keratinocytes were treated with DSW or saline MW. CGRP and SFRP4 expression levels were assessed alongside autophagy markers beclin-1 and LC3B. The involvement of autophagy was confirmed using wortmannin, an autophagy inhibitor.
Results: Both DSW (4.7 ± 1.9 a.u.) and saline MW (3.6 ± 1.6 a.u.) significantly reduced CGRP expression compared to controls (non-magnetized saline: 7.5 ± 2.3 a.u.; distilled water: 7.6 ± 2.5 a.u.; all p< 0.001). While both fluids enhanced SFRP4 expression equally (p = 0.78), saline MW showed superior CGRP inhibition (p< 0.001). Both fluids mitigated polycytokine-induced reductions in beclin-1 and LC3B levels (all p< 0.001), with saline MW showing more pronounced effects (p< 0.05). Wortmannin impaired the effects of both fluids on CGRP and SFRP4, indicating autophagy mediation.
Conclusions: DSW and saline MW show promise as sustainable active ingredients for topical formulations targeting psoriatic inflammation via autophagy activation.