心血管疾病的内皮功能障碍:机制和体外模型。

IF 3.7 2区 生物学 Q3 CELL BIOLOGY
Molecular and Cellular Biochemistry Pub Date : 2025-08-01 Epub Date: 2025-04-21 DOI:10.1007/s11010-025-05289-w
Ana Grego, Cristiana Fernandes, Ivo Fonseca, Marina Dias-Neto, Raquel Costa, Adelino Leite-Moreira, Sandra Marisa Oliveira, Fábio Trindade, Rita Nogueira-Ferreira
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引用次数: 0

摘要

内皮细胞(ECs)并排排列,形成半透性单层,形成每根血管(微循环和大循环)的内层。作为循环分子和细胞的第一道屏障,内皮细胞代表了血管稳态的主要调节剂,能够通过产生几种调节血管张力和细胞粘附的因子来响应环境变化,无论是物理信号还是化学信号。健康的内皮具有抗凝特性,可以防止白细胞和血小板粘附到血管壁上,有助于抵抗血栓形成,调节炎症和血管平滑肌细胞增殖。许多心血管疾病(cvd)的危险因素促进内皮细胞表达趋化因子、细胞因子和粘附分子。由此产生的内皮细胞激活可导致内皮细胞功能障碍(ECD)。体外ECD模型允许研究疾病的细胞和分子机制,并为筛选潜在的治疗药物提供研究平台。尽管存在其他模型,如动物模型或离体模型,但体外模型提供了更高的实验灵活性和可重复性,使其成为了解心血管疾病等几种疾病病理生理机制的宝贵工具。因此,本文旨在综合目前有关ECD的体外模型,重点介绍cvd。这项工作将侧重于2D细胞培养模型(内皮细胞系和原代内皮细胞)、3D细胞培养系统(无支架和基于支架)和3D细胞培养模型(如器官芯片)。我们将剖析外部刺激-化学和机械-在触发ECD中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Endothelial dysfunction in cardiovascular diseases: mechanisms and in vitro models.

Endothelial dysfunction in cardiovascular diseases: mechanisms and in vitro models.

Endothelial dysfunction in cardiovascular diseases: mechanisms and in vitro models.

Endothelial dysfunction in cardiovascular diseases: mechanisms and in vitro models.

Endothelial cells (ECs) are arranged side-by-side to create a semi-permeable monolayer, forming the inner lining of every blood vessel (micro and macrocirculation). Serving as the first barrier for circulating molecules and cells, ECs represent the main regulators of vascular homeostasis being able to respond to environmental changes, either physical or chemical signals, by producing several factors that regulate vascular tone and cellular adhesion. Healthy endothelium has anticoagulant properties that prevent the adhesion of leukocytes and platelets to the vessel walls, contributing to resistance to thrombus formation, and regulating inflammation, and vascular smooth muscle cell proliferation. Many risk factors of cardiovascular diseases (CVDs) promote the endothelial expression of chemokines, cytokines, and adhesion molecules. The resultant endothelial activation can lead to endothelial cell dysfunction (ECD). In vitro models of ECD allow the study of cellular and molecular mechanisms of disease and provide a research platform for screening potential therapeutic agents. Even though alternative models are available, such as animal models or ex vivo models, in vitro models offer higher experimental flexibility and reproducibility, making them a valuable tool for the understanding of pathophysiological mechanisms of several diseases, such as CVDs. Therefore, this review aims to synthesize the currently available in vitro models regarding ECD, emphasizing CVDs. This work will focus on 2D cell culture models (endothelial cell lines and primary ECs), 3D cell culture systems (scaffold-free and scaffold-based), and 3D cell culture models (such as organ-on-a-chip). We will dissect the role of external stimuli-chemical and mechanical-in triggering ECD.

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来源期刊
Molecular and Cellular Biochemistry
Molecular and Cellular Biochemistry 生物-细胞生物学
CiteScore
8.30
自引率
2.30%
发文量
293
审稿时长
1.7 months
期刊介绍: Molecular and Cellular Biochemistry: An International Journal for Chemical Biology in Health and Disease publishes original research papers and short communications in all areas of the biochemical sciences, emphasizing novel findings relevant to the biochemical basis of cellular function and disease processes, as well as the mechanics of action of hormones and chemical agents. Coverage includes membrane transport, receptor mechanism, immune response, secretory processes, and cytoskeletal function, as well as biochemical structure-function relationships in the cell. In addition to the reports of original research, the journal publishes state of the art reviews. Specific subjects covered by Molecular and Cellular Biochemistry include cellular metabolism, cellular pathophysiology, enzymology, ion transport, lipid biochemistry, membrane biochemistry, molecular biology, nuclear structure and function, and protein chemistry.
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