羟基红花黄A通过靶向Notch1信号通路对Jurkat细胞增殖、细胞周期和凋亡的影响

IF 2 4区 医学 Q3 PHYSIOLOGY
Journal of Physiology and Pharmacology Pub Date : 2025-04-01 Epub Date: 2025-05-05 DOI:10.26402/jpp.2025.2.07
X-Y Hao, X-L Liu, J-Y Wang, A-M Li, X-L Wang
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引用次数: 0

摘要

本研究旨在探讨羟基红花黄A (HSYA)对t细胞急性淋巴细胞白血病(T-ALL)的代表模型Jurkat细胞增殖、细胞周期和凋亡的影响,并探讨其作用的潜在分子机制。给予Jurkat细胞HSYA。采用细胞计数试剂盒-8 (CCK-8)检测细胞增殖,流式细胞术分析细胞周期分布和凋亡情况。采用逆转录-定量聚合酶链反应(RT-qPCR)和Western blot技术评估Notch1、c-Myc和Hes1 mRNA和蛋白水平的表达水平。HSYA阻碍细胞生长的方式取决于时间和剂量。HSYA在24、48和72 h时对Jurkat细胞的IC50(半最大抑制浓度)分别为99.08、77.81和59.05 μmol/L。HSYA诱导Jurkat细胞G0/G1细胞周期阻滞呈浓度依赖性。不同浓度HSYA处理48h后,G1期细胞数量显著增加(F=48.588, df=2, P
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impact of hydroxysafflor yellow A on proliferation, cell cycle, and apoptosis of Jurkat cells through targeting of the Notch1 signaling pathway.

This study aimed to explore the impact of hydroxysafflor yellow A (HSYA) on the proliferation, cell cycle, and apoptosis of Jurkat cells, serving as a representative model of T-cell acute lymphoblastic leukemia (T-ALL), and to explore the underlying molecular mechanism of its action. HSYA was administered to Jurkat cells. Cell proliferation was assessed using the Cell Counting Kit-8 (CCK-8) assay, and flow cytometry was utilized to analyze cell cycle distribution and apoptosis. The expression levels of Notch1, c-Myc, and Hes1 at both mRNA and protein levels were assessed employing reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blot techniques. HSYA hinders cell growth in a manner dependent on both time and dosage. The IC50 (half-maximal inhibitory concentration) for HSYA to inhibit Jurkat cells was 99.08 μmol/L, 77.81 μmol/L and 59.05 μmol/L at 24, 48, and 72 hours, respectively. HSYA induced G0/G1 cell cycle arrest in Jurkat cells in a concentration-dependent manner. After exposure to different concentration of HSYA for 48 hours there was a notable increase in G1 phase cells (F=48.588, df=2, P<0.001) accompanied by decrease in S phase cells (F=66.637, df=2, P<0.001). After 48 hours of HSYA treatment, apoptosis in the experimental group was significantly up-regulated compared with the control group (F=98.09, df=2, P<0.001). After 48 hours of HSYA treatment with varying concentrations (50 and 100 μmol/L), the Jurkat cells exhibited significantly lower expression levels of Notch1, c-Myc, and Hes1 mRNA as compared to the control group (F=298.361, df=2, P<0.001 for comparison of Notch1 expression; F=173.332, df=2, P<0.001 for comparison of c-Myc expression; F=126.563, df=2, P<0.001 for comparison of Hes1 expression). HSYA can play an anti-leukemia effect by inhibiting Notch1 signaling pathway in T-ALL, and can be as a potential candidate for the treatment of T-ALL.

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来源期刊
CiteScore
4.00
自引率
22.70%
发文量
0
审稿时长
6-12 weeks
期刊介绍: Journal of Physiology and Pharmacology publishes papers which fall within the range of basic and applied physiology, pathophysiology and pharmacology. The papers should illustrate new physiological or pharmacological mechanisms at the level of the cell membrane, single cells, tissues or organs. Clinical studies, that are of fundamental importance and have a direct bearing on the pathophysiology will also be considered. Letters related to articles published in The Journal with topics of general professional interest are welcome.
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