测定黄热病病毒中和抗体（Nab）的微焦点减少中和试验（mFRNT）

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4458-4_17

Adriana de Souza Azevedo, Nathalia Dos Santos Alves

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引用次数: 0

摘要

虽然斑块减少中和试验（PRNT）被认为是测量黄热病病毒（YFV）中和抗体的参考，但微焦点减少中和试验（mFRNT）具有许多优点，包括更高的吞吐量，更短的孵育时间，并允许自动焦点计数。因此，我们在这里描述了一种详细的mFRNT方法，它为非临床和临床研究以及疫苗设计提供了一种更快的中和试验。

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Micro-Focus Reduction Neutralization Tests (mFRNT) for Neutralizing Antibody (Nab) Quantification Against Yellow Fever Virus.

Although the plaque reduction neutralization test (PRNT) is considered the reference for measuring neutralizing antibodies for Yellow fever virus (YFV), the micro-focus reduction neutralization test (mFRNT) presents many advantages including a higher throughput, a reduced incubation time, and allows the automatic foci count. So, here we describe a detailed mFRNT method that provides a faster neutralization test essential for non-clinical and clinical studies and vaccine design.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.