circ_0046599通过与miR-1322竞争增强NT5DC2表达促进HCC进展。

IF 3.3 3区 医学 Q2 ONCOLOGY
Journal of Cancer Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI:10.7150/jca.103661
Xiaobo Zhang, Suxin Li, Luhao Li, Dingyang Li, Huashan Zhao, Xiaole Gao, Xiaowei Dang
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引用次数: 0

摘要

背景:本研究旨在探讨circ_0046599对肝细胞癌(HCC)的影响。方法:分析GEO数据集,确定circ_0046599在HCC中显著上调,并评估其对细胞增殖、凋亡、迁移和侵袭的影响。生物信息学和双荧光素酶检测发现miR-1322是circ_0046599的靶标,而circ_0046599反过来调节NT5DC2的表达。体外和体内实验证实了circ_0046599在HCC中的ceRNA机制。结果:circ_0046599在HCC中显著上调,并预测HCC患者的生存率较差。circ_0046599表达增加,促进HCC细胞增殖、迁移、侵袭,抑制细胞凋亡。circ_0046599与miR-1322结合,在HCC细胞中发挥肿瘤抑制作用。miR-1322靶向NT5DC2, circ_0046599通过与miR-1322竞争性结合调控NT5DC2的表达。NT5DC2表达的调控影响circ_0046599的致癌作用。在体内实验中,抑制circ_0046599通过上调miR-1322表达和抑制NT5DC2来抑制异种移植肿瘤的生长。结论:circ_0046599通过与miR-1322竞争性结合,调控NT5DC2的表达,促进HCC的进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
circ_0046599 Promotes HCC Progression by Competing with miR-1322 to Enhance NT5DC2 Expression.

Background: This study aimed to investigate the impact of circ_0046599 on hepatocellular carcinoma (HCC). Methods: Analysis of the GEO dataset identified circ_0046599 as significantly upregulated in HCC, and its impact on cell proliferation, apoptosis, migration, and invasion was assessed. Bioinformatics and dual-luciferase assays identified miR-1322 as a target of circ_0046599, which in turn regulates NT5DC2 expression. In vitro and in vivo experiments confirmed the ceRNA mechanism of circ_0046599 in HCC. Results: circ_0046599 was significantly upregulated in HCC, and predicts a worse survival in HCC patients. Increased expression of circ_0046599 promoted HCC cell proliferation, migration, invasion, and inhibited apoptosis. circ_0046599 bound to miR-1322, which exerted a tumor-suppressive effect in HCC cells. miR-1322 targeted NT5DC2, and circ_0046599 regulated the expression of NT5DC2 by competitively binding to miR-1322. Modulation of NT5DC2 expression affected the oncogenic role of circ_0046599. In in vivo experiments, inhibition of circ_0046599 suppressed the growth of xenograft tumors by upregulating miR-1322 expression and suppressing NT5DC2. Conclusion: circ_0046599 promoted the progression of HCC by competitively binding to miR-1322 and regulating the expression of NT5DC2.

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来源期刊
Journal of Cancer
Journal of Cancer ONCOLOGY-
CiteScore
8.10
自引率
2.60%
发文量
333
审稿时长
12 weeks
期刊介绍: Journal of Cancer is an open access, peer-reviewed journal with broad scope covering all areas of cancer research, especially novel concepts, new methods, new regimens, new therapeutic agents, and alternative approaches for early detection and intervention of cancer. The Journal is supported by an international editorial board consisting of a distinguished team of cancer researchers. Journal of Cancer aims at rapid publication of high quality results in cancer research while maintaining rigorous peer-review process.
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