莫里素水合物降低了埃及伊蚊的存活率和繁殖力,延缓了发育并削弱了脂质储备。

IF 1.6 3区 农林科学 Q2 ENTOMOLOGY
Luan Valim Dos Santos, Elaine Rodrigues Miranda Nery da Silva, Matheus Silva Caiado, Renan Albuquerque Camasmie, Herbert Marcusi Souza de Agustini, Raquel do Nascimento de Souza, Bruno Guimarães Marinho, Rosane Nora Castro, Mario Geraldo de Carvalho, Emerson Guedes Pontes
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引用次数: 0

摘要

埃及伊蚊通常与引起黄热病、登革热、寨卡和基孔肯雅热的虫媒病毒有关。控制其种群的最有效方法是在繁殖地使用剧毒杀虫剂,这些杀虫剂也可以影响非目标生物并产生抗性种群。因此,化合物的使用是可取的。除了对哺乳动物没有负面影响外,水合Morin还具有广泛的抗氧化潜力,具有广泛的药理应用。因此,本研究旨在探讨水合桑苷对埃及伊蚊幼虫暴露24 h后存活率、化蛹率、产卵量、甘油三酯储备及脂质代谢相关蛋白表达的影响。为此,配制了含有不同浓度水合桑里酯的埃及伊蚊幼虫饲养培养基,以评价其致死浓度。LC25为52.692 μM, LC40为111.121 μM, LC50为174.775 μM, LC75为575.083 μM, LC90为1685.936 μM。用水合桑酸处理24 h后,将存活幼虫与食物一起转移到不含桑酸的水中,观察其化蛹率和育性。我们观察到,当浓度超过100 μM时,水合桑苷浓度的增加会引起成虫存活率的剂量依赖性降低,使成虫化蛹时间增加一倍,并使幼虫期处理过的雌虫产卵数量减少约30%。由此评估24 h对甘油三酯(TAG)储备的影响,并评估脂质代谢相关蛋白的表达。100 μM morin处理24 h后,幼虫的TAG储量减少了约17%,175 μM时,存量减少了33%以上,500 μM时,存量减少了61%。此外,脂溶蛋白TAGL1和HSL上调,而脂肪生成蛋白FAS1、DGAT1和GPAT1下调。胰岛素样受体也下调,不像AKHr也上调。这些数据表明,水合物桑里素通过影响埃及伊蚊的脂质代谢来降低其存活率和繁殖力。水合物莫里酯对非目标生物没有毒性,显示出控制蚊子种群的有趣潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Morin hydrate reduces survival and fertility, delays development and weakens lipid reserves in Aedes aegypti.

The Aedes aegypti mosquito is generally associated with arboviruses that cause yellow fever, dengue, zika and chikungunya. The most efficient way to control their populations is through application in breeding sites of highly toxic insecticides that can also impact non-target organisms and generate resistant populations. Therefore, the use of compounds is desirable. Morin hydrate has broad pharmacological applications based on its antioxidant potential, in addition to not having negative effects on mammals. Therefore, the objective of the present study was to investigate the effects of morin hydrate on A. aegypti survival, pupation rate, egg laying, triacylglycerol reserves and expression of proteins related to lipid metabolism 24 h after exposure of larvae. For this, rearing media containing A. aegypti larvae with different concentrations of morin hydrate were formulated to evaluate the lethal concentration. Calculation of the expected lethal concentrations showed LC25 of 52.692 μM, LC40 of 111.121 μM, LC50 of 174.775 μM, LC75 of 575.083 μM and LC90 of 1685.936 μM. Twenty-four hours after treatment with morin hydrate, surviving larvae were transferred to morin-free water with food, and their pupation rate and fertility were evaluated. We observed that an increase in the concentration of morin hydrate induced a dose-dependent reduction in survival, doubled pupation time in survivors and reduced the number of eggs laid by treated females during the larval stage by approximately 30% at concentrations exceeding 100 μM. From this, the impact of 24 h on the triacylglycerol (TAG) stock was evaluated, in addition to evaluating the expression of proteins involved in lipid metabolism. Larvae 24 h after treatment with 100 μM morin showed a reduction in TAG reserves of approximately 17%, while at 175 μM, there was a reduction of more than 33% in stocks, and at 500 μM there was a reduction of 61%. Furthermore, the lipolytic proteins TAGL1 and HSL were upregulated, while the lipogenic proteins FAS1, DGAT1 and GPAT1 were downregulated. Insulin-like receptors were also downregulated, unlike AKHr, which was also upregulated. These data demonstrate that morin hydrate reduces the survival and fertility of A. aegypti by affecting its lipid metabolism. Morin hydrate did not exhibit toxicity toward non-target organisms, demonstrating interesting potential for the control of mosquito populations.

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来源期刊
Medical and Veterinary Entomology
Medical and Veterinary Entomology 农林科学-昆虫学
CiteScore
3.70
自引率
5.30%
发文量
65
审稿时长
12-24 weeks
期刊介绍: Medical and Veterinary Entomology is the leading periodical in its field. The Journal covers the biology and control of insects, ticks, mites and other arthropods of medical and veterinary importance. The main strengths of the Journal lie in the fields of: -epidemiology and transmission of vector-borne pathogens changes in vector distribution that have impact on the pathogen transmission- arthropod behaviour and ecology- novel, field evaluated, approaches to biological and chemical control methods- host arthropod interactions. Please note that we do not consider submissions in forensic entomology.
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