{"title":"紫外光谱法测定别嘌呤醇和番茄红素的生物活性配方和体积。","authors":"Soumya Mishra, Ranjit K Harwansh, Rupa Mazumder","doi":"10.2174/0118723128322443240919025352","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>This study developed a UV spectrophotometric method for quantifying allopurinol and lycopene in both bulk and dosage forms, aiming for simplicity and practicality.</p><p><strong>Materials and methods: </strong>The method was developed and validated using methanol and lycopene in phosphate buffer saline at various pH levels (7.4, 6.8, 1.2, 5.5). Validation parameters included linearity, accuracy, precision, limit of quantification (LOQ), and limit of detection (LOD) according to ICH standards.</p><p><strong>Results: </strong>The method demonstrated high sensitivity and linearity over a concentration range of 4-20 μg/ml, adhering to Beer's law. The LOQ and LOD were 2-18 μg/ml and 2-6 μg/ml, respectively. Optimal absorbance wavelengths were 251 nm for lycopene and 471 nm for allopurinol. Calibration curves showed a high correlation coefficient (0.9994), indicating a strong linear relationship.</p><p><strong>Conclusion: </strong>The developed UV spectrophotometric method is effective, interference-free, and suitable for routine quality control, providing reliable measurements for allopurinol and lycopene.</p>","PeriodicalId":72844,"journal":{"name":"Drug metabolism and bioanalysis letters","volume":"17 2","pages":"56-66"},"PeriodicalIF":0.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bioactive Formulation and Bulk Allopurinol and Lycopene Estimation by UV Spectroscopy.\",\"authors\":\"Soumya Mishra, Ranjit K Harwansh, Rupa Mazumder\",\"doi\":\"10.2174/0118723128322443240919025352\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>This study developed a UV spectrophotometric method for quantifying allopurinol and lycopene in both bulk and dosage forms, aiming for simplicity and practicality.</p><p><strong>Materials and methods: </strong>The method was developed and validated using methanol and lycopene in phosphate buffer saline at various pH levels (7.4, 6.8, 1.2, 5.5). Validation parameters included linearity, accuracy, precision, limit of quantification (LOQ), and limit of detection (LOD) according to ICH standards.</p><p><strong>Results: </strong>The method demonstrated high sensitivity and linearity over a concentration range of 4-20 μg/ml, adhering to Beer's law. The LOQ and LOD were 2-18 μg/ml and 2-6 μg/ml, respectively. Optimal absorbance wavelengths were 251 nm for lycopene and 471 nm for allopurinol. Calibration curves showed a high correlation coefficient (0.9994), indicating a strong linear relationship.</p><p><strong>Conclusion: </strong>The developed UV spectrophotometric method is effective, interference-free, and suitable for routine quality control, providing reliable measurements for allopurinol and lycopene.</p>\",\"PeriodicalId\":72844,\"journal\":{\"name\":\"Drug metabolism and bioanalysis letters\",\"volume\":\"17 2\",\"pages\":\"56-66\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Drug metabolism and bioanalysis letters\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2174/0118723128322443240919025352\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Drug metabolism and bioanalysis letters","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/0118723128322443240919025352","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Bioactive Formulation and Bulk Allopurinol and Lycopene Estimation by UV Spectroscopy.
Background: This study developed a UV spectrophotometric method for quantifying allopurinol and lycopene in both bulk and dosage forms, aiming for simplicity and practicality.
Materials and methods: The method was developed and validated using methanol and lycopene in phosphate buffer saline at various pH levels (7.4, 6.8, 1.2, 5.5). Validation parameters included linearity, accuracy, precision, limit of quantification (LOQ), and limit of detection (LOD) according to ICH standards.
Results: The method demonstrated high sensitivity and linearity over a concentration range of 4-20 μg/ml, adhering to Beer's law. The LOQ and LOD were 2-18 μg/ml and 2-6 μg/ml, respectively. Optimal absorbance wavelengths were 251 nm for lycopene and 471 nm for allopurinol. Calibration curves showed a high correlation coefficient (0.9994), indicating a strong linear relationship.
Conclusion: The developed UV spectrophotometric method is effective, interference-free, and suitable for routine quality control, providing reliable measurements for allopurinol and lycopene.
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