Elijah Kolawole Oladipo, Stephen Feranmi Adeyemo, Ayomiposi Isaiah Oshoneye, Hannah Blessing Akintola, Bolatito Islam Elegbede, Tobiloba Uren Ayoomoba, Dorcas Ayomide Atilade, Omolara Omoboye Adegboye, Abuoma Elizabeth Ejikeme, Chris Olamide Balogun, Kehinde Abolade Aderibigbe, Possible Okikiola Popoola, Victoria Ajike Alabi, Boluwatife Ayobami Irewolede, Gbemi Henry Ano-Edward, Ademola Olabode Ayeleso, Helen Onyeaka
{"title":"利用计算免疫学设计家禽传染性法氏囊病靶向亚单位疫苗。","authors":"Elijah Kolawole Oladipo, Stephen Feranmi Adeyemo, Ayomiposi Isaiah Oshoneye, Hannah Blessing Akintola, Bolatito Islam Elegbede, Tobiloba Uren Ayoomoba, Dorcas Ayomide Atilade, Omolara Omoboye Adegboye, Abuoma Elizabeth Ejikeme, Chris Olamide Balogun, Kehinde Abolade Aderibigbe, Possible Okikiola Popoola, Victoria Ajike Alabi, Boluwatife Ayobami Irewolede, Gbemi Henry Ano-Edward, Ademola Olabode Ayeleso, Helen Onyeaka","doi":"10.3389/fbinf.2025.1562997","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Infectious bursal disease (IBD), caused by the infectious bursal disease Q8 virus (IBDV), is a highly contagious disease in young chickens, leading to immunosuppression with great economic importance. IBDV, a non-enveloped virus with a bipartite dsRNA genome, infects the bursa of Fabricius, causing severe gastrointestinal disease. Effective vaccines are urgently needed due to the limitations of current oral vaccines, including gastrointestinal degradation and low immunogenicity. This study designs and evaluates a multiepitope subunit vaccine using immunoinformatics.</p><p><strong>Methods: </strong>Sequences of the IBDV structural proteins VP2 and VP3 were obtained from the National Centre for Biotechnology Information) NCBI. These are structural proteins VP2 and VP3 were subjected to the Vaxijen 2.0 webserver to predict the antigenicity, ToxiPred to predict the toxicity and further analyzed to identify immunogenic epitopes of Chicken Leukocyte Antigens (CLAs) using the NetMHCpan 4.1 webserver.</p><p><strong>Results: </strong>The final vaccine construct includes 2 HTL, 21 CTL, and 7 LBL epitopes, with gallinacin-3 precursor as an adjuvant. The construct is antigenic (0.5605), non-allergenic, and non-toxic, consisting of 494 amino acids with a molecular weight of 54.88 kDa and a positive charge (pI of 9.23). It is stable, hydrophilic, and soluble. Population coverage analysis revealed a global immune coverage of 89.83%, with the highest in Europe (99.86%) and the lowest in Central America (25.01%). Molecular docking revealed strong interactions with TLR-2_1, TLR-4, and TLR-7, with TLR-7 exhibiting the highest binding affinity (-366.15 kcal/mol). Immune simulations indicated a robust immune response, with high initial IgM levels, sustained IgG, memory cell formation, and activation of T helper (Th) cells 1 and 2, Natural Killer (NK) cells, and dendritic cells, suggesting potential long-lasting immunity against IBDV.</p><p><strong>Discussion: </strong>This study presents a promising multi-epitope subunit vaccine candidate capable of effective immunization against IBDV with broad population coverage. However, further <i>in vivo</i> experimental validation is required to confirm its efficacy and safety.</p>","PeriodicalId":73066,"journal":{"name":"Frontiers in bioinformatics","volume":"5 ","pages":"1562997"},"PeriodicalIF":2.8000,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006097/pdf/","citationCount":"0","resultStr":"{\"title\":\"Harnessing computational immunology to design targeted subunit vaccines for infectious bursal disease in poultry.\",\"authors\":\"Elijah Kolawole Oladipo, Stephen Feranmi Adeyemo, Ayomiposi Isaiah Oshoneye, Hannah Blessing Akintola, Bolatito Islam Elegbede, Tobiloba Uren Ayoomoba, Dorcas Ayomide Atilade, Omolara Omoboye Adegboye, Abuoma Elizabeth Ejikeme, Chris Olamide Balogun, Kehinde Abolade Aderibigbe, Possible Okikiola Popoola, Victoria Ajike Alabi, Boluwatife Ayobami Irewolede, Gbemi Henry Ano-Edward, Ademola Olabode Ayeleso, Helen Onyeaka\",\"doi\":\"10.3389/fbinf.2025.1562997\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Infectious bursal disease (IBD), caused by the infectious bursal disease Q8 virus (IBDV), is a highly contagious disease in young chickens, leading to immunosuppression with great economic importance. IBDV, a non-enveloped virus with a bipartite dsRNA genome, infects the bursa of Fabricius, causing severe gastrointestinal disease. Effective vaccines are urgently needed due to the limitations of current oral vaccines, including gastrointestinal degradation and low immunogenicity. This study designs and evaluates a multiepitope subunit vaccine using immunoinformatics.</p><p><strong>Methods: </strong>Sequences of the IBDV structural proteins VP2 and VP3 were obtained from the National Centre for Biotechnology Information) NCBI. These are structural proteins VP2 and VP3 were subjected to the Vaxijen 2.0 webserver to predict the antigenicity, ToxiPred to predict the toxicity and further analyzed to identify immunogenic epitopes of Chicken Leukocyte Antigens (CLAs) using the NetMHCpan 4.1 webserver.</p><p><strong>Results: </strong>The final vaccine construct includes 2 HTL, 21 CTL, and 7 LBL epitopes, with gallinacin-3 precursor as an adjuvant. The construct is antigenic (0.5605), non-allergenic, and non-toxic, consisting of 494 amino acids with a molecular weight of 54.88 kDa and a positive charge (pI of 9.23). It is stable, hydrophilic, and soluble. Population coverage analysis revealed a global immune coverage of 89.83%, with the highest in Europe (99.86%) and the lowest in Central America (25.01%). Molecular docking revealed strong interactions with TLR-2_1, TLR-4, and TLR-7, with TLR-7 exhibiting the highest binding affinity (-366.15 kcal/mol). Immune simulations indicated a robust immune response, with high initial IgM levels, sustained IgG, memory cell formation, and activation of T helper (Th) cells 1 and 2, Natural Killer (NK) cells, and dendritic cells, suggesting potential long-lasting immunity against IBDV.</p><p><strong>Discussion: </strong>This study presents a promising multi-epitope subunit vaccine candidate capable of effective immunization against IBDV with broad population coverage. 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Harnessing computational immunology to design targeted subunit vaccines for infectious bursal disease in poultry.
Introduction: Infectious bursal disease (IBD), caused by the infectious bursal disease Q8 virus (IBDV), is a highly contagious disease in young chickens, leading to immunosuppression with great economic importance. IBDV, a non-enveloped virus with a bipartite dsRNA genome, infects the bursa of Fabricius, causing severe gastrointestinal disease. Effective vaccines are urgently needed due to the limitations of current oral vaccines, including gastrointestinal degradation and low immunogenicity. This study designs and evaluates a multiepitope subunit vaccine using immunoinformatics.
Methods: Sequences of the IBDV structural proteins VP2 and VP3 were obtained from the National Centre for Biotechnology Information) NCBI. These are structural proteins VP2 and VP3 were subjected to the Vaxijen 2.0 webserver to predict the antigenicity, ToxiPred to predict the toxicity and further analyzed to identify immunogenic epitopes of Chicken Leukocyte Antigens (CLAs) using the NetMHCpan 4.1 webserver.
Results: The final vaccine construct includes 2 HTL, 21 CTL, and 7 LBL epitopes, with gallinacin-3 precursor as an adjuvant. The construct is antigenic (0.5605), non-allergenic, and non-toxic, consisting of 494 amino acids with a molecular weight of 54.88 kDa and a positive charge (pI of 9.23). It is stable, hydrophilic, and soluble. Population coverage analysis revealed a global immune coverage of 89.83%, with the highest in Europe (99.86%) and the lowest in Central America (25.01%). Molecular docking revealed strong interactions with TLR-2_1, TLR-4, and TLR-7, with TLR-7 exhibiting the highest binding affinity (-366.15 kcal/mol). Immune simulations indicated a robust immune response, with high initial IgM levels, sustained IgG, memory cell formation, and activation of T helper (Th) cells 1 and 2, Natural Killer (NK) cells, and dendritic cells, suggesting potential long-lasting immunity against IBDV.
Discussion: This study presents a promising multi-epitope subunit vaccine candidate capable of effective immunization against IBDV with broad population coverage. However, further in vivo experimental validation is required to confirm its efficacy and safety.
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