Ao Liu, Tianlin Sun, Tong Qiu, Yunqing Chen, Huiyang Qi, Wenxing Du, Zhe Wu, Zhan Huang, Wenqing Su, Changbin Zhu, Wenjie Jiao
{"title":"CXCL1和CXCL8:鉴别非小细胞肺癌肺内转移和多种原发肿瘤的可靠和可行的生物标志物。","authors":"Ao Liu, Tianlin Sun, Tong Qiu, Yunqing Chen, Huiyang Qi, Wenxing Du, Zhe Wu, Zhan Huang, Wenqing Su, Changbin Zhu, Wenjie Jiao","doi":"10.1177/18758592241308730","DOIUrl":null,"url":null,"abstract":"<p><p>ObjectiveIn NSCLC, the main approach to differentiate between intrapulmonary metastases (IPM) and multiple primary lung cancer (MPLC) is to integrate histopathological and genomic information. Here, we identified viable biomarkers that can distinguish IPM from MPLC by integrating comprehensive genomic profiling (CGP) and targeted RNA sequencing.MethodsWe retrospectively collected tissues from at least two lesions in 34 patients. 29 and 5 out of 34 patients determined as pathologic MPLC (pMPLC) and pathologic IPM, respectively, according to Martini-Melamed criteria (M-M criteria). A comprehensive investigation at genomic and transcriptomic level was conducted.ResultsNine of the 29 pMPLCs shared trunk mutations in their lesions and were consequently reclassified as IPM. Survival analyses revealed that classification integrated M-M criteria and mutational profiling could distinguish IPM/MPLC more accurately. Further exploration at the transcriptomic level revealed elevated expression levels of genes related to epithelial-mesenchymal transition and immunomodulatory pathways in IPM. Notably, the expression of <i>CXCL1</i> and <i>CXCL8</i> was significantly upregulated in IPM.ConclusionsWe found that the expression of <i>CXCL1</i> and <i>CXCL8</i> in any tumor lesion within a patient could reliably indicate IPM. Additionally, assessing the transcriptional levels of <i>CXCL1</i> and <i>CXCL8</i> also provide a dependable and practical approach to identify IPM from MPLC.</p>","PeriodicalId":56320,"journal":{"name":"Cancer Biomarkers","volume":"42 4","pages":"18758592241308730"},"PeriodicalIF":2.2000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"CXCL1 and CXCL8: Reliable and feasible biomarkers differentiating intrapulmonary metastasis from multiple primary neoplasms in non-small cell lung cancers.\",\"authors\":\"Ao Liu, Tianlin Sun, Tong Qiu, Yunqing Chen, Huiyang Qi, Wenxing Du, Zhe Wu, Zhan Huang, Wenqing Su, Changbin Zhu, Wenjie Jiao\",\"doi\":\"10.1177/18758592241308730\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>ObjectiveIn NSCLC, the main approach to differentiate between intrapulmonary metastases (IPM) and multiple primary lung cancer (MPLC) is to integrate histopathological and genomic information. Here, we identified viable biomarkers that can distinguish IPM from MPLC by integrating comprehensive genomic profiling (CGP) and targeted RNA sequencing.MethodsWe retrospectively collected tissues from at least two lesions in 34 patients. 29 and 5 out of 34 patients determined as pathologic MPLC (pMPLC) and pathologic IPM, respectively, according to Martini-Melamed criteria (M-M criteria). A comprehensive investigation at genomic and transcriptomic level was conducted.ResultsNine of the 29 pMPLCs shared trunk mutations in their lesions and were consequently reclassified as IPM. Survival analyses revealed that classification integrated M-M criteria and mutational profiling could distinguish IPM/MPLC more accurately. Further exploration at the transcriptomic level revealed elevated expression levels of genes related to epithelial-mesenchymal transition and immunomodulatory pathways in IPM. Notably, the expression of <i>CXCL1</i> and <i>CXCL8</i> was significantly upregulated in IPM.ConclusionsWe found that the expression of <i>CXCL1</i> and <i>CXCL8</i> in any tumor lesion within a patient could reliably indicate IPM. Additionally, assessing the transcriptional levels of <i>CXCL1</i> and <i>CXCL8</i> also provide a dependable and practical approach to identify IPM from MPLC.</p>\",\"PeriodicalId\":56320,\"journal\":{\"name\":\"Cancer Biomarkers\",\"volume\":\"42 4\",\"pages\":\"18758592241308730\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cancer Biomarkers\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1177/18758592241308730\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/4/15 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"ONCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cancer Biomarkers","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/18758592241308730","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/15 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ONCOLOGY","Score":null,"Total":0}
CXCL1 and CXCL8: Reliable and feasible biomarkers differentiating intrapulmonary metastasis from multiple primary neoplasms in non-small cell lung cancers.
ObjectiveIn NSCLC, the main approach to differentiate between intrapulmonary metastases (IPM) and multiple primary lung cancer (MPLC) is to integrate histopathological and genomic information. Here, we identified viable biomarkers that can distinguish IPM from MPLC by integrating comprehensive genomic profiling (CGP) and targeted RNA sequencing.MethodsWe retrospectively collected tissues from at least two lesions in 34 patients. 29 and 5 out of 34 patients determined as pathologic MPLC (pMPLC) and pathologic IPM, respectively, according to Martini-Melamed criteria (M-M criteria). A comprehensive investigation at genomic and transcriptomic level was conducted.ResultsNine of the 29 pMPLCs shared trunk mutations in their lesions and were consequently reclassified as IPM. Survival analyses revealed that classification integrated M-M criteria and mutational profiling could distinguish IPM/MPLC more accurately. Further exploration at the transcriptomic level revealed elevated expression levels of genes related to epithelial-mesenchymal transition and immunomodulatory pathways in IPM. Notably, the expression of CXCL1 and CXCL8 was significantly upregulated in IPM.ConclusionsWe found that the expression of CXCL1 and CXCL8 in any tumor lesion within a patient could reliably indicate IPM. Additionally, assessing the transcriptional levels of CXCL1 and CXCL8 also provide a dependable and practical approach to identify IPM from MPLC.
期刊介绍:
Concentrating on molecular biomarkers in cancer research, Cancer Biomarkers publishes original research findings (and reviews solicited by the editor) on the subject of the identification of markers associated with the disease processes whether or not they are an integral part of the pathological lesion.
The disease markers may include, but are not limited to, genomic, epigenomic, proteomics, cellular and morphologic, and genetic factors predisposing to the disease or indicating the occurrence of the disease. Manuscripts on these factors or biomarkers, either in altered forms, abnormal concentrations or with abnormal tissue distribution leading to disease causation will be accepted.