有丝分裂过程中CEP215/CDK5RAP2对纺锤杆组装的动力学增强。

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Journal of cell science Pub Date : 2025-05-15 Epub Date: 2025-05-21 DOI:10.1242/jcs.263542
Hyunjeong Yoo, Taehyun Kim, Sungjin Ryu, Donghee Ko, Jeesoo Kim, Hee-Jung Choi, Yongdae Shin, Kunsoo Rhee
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引用次数: 0

摘要

中心体的微管组织活性在细胞周期中波动,在M期达到最高水平。CEP215/CDK5RAP2是人类中心体微管组织的关键中心周围物质(PCM)蛋白。在这里,我们提供的证据表明,CEP215在间期中心体中表现出一种动态抑制的固体状状态,而在有丝分裂中心体中则变得更加动态。与另一中心体蛋白PCNT的特异性相互作用对于CEP215蛋白的扩散分子动力学至关重要。我们还发现,在光诱导体系中,当CEP215的线圈结构域(CCDs)被截断时,它的簇形成活性会受到损害。在CEP215被ccp截断的突变体所取代的细胞中,纺锤极的组装和纺锤体的形成都出现了缺陷。我们的研究结果支持了CEP215的扩散迁移率通过ccd之间的同型和异型相互作用而增强,特别是在有丝分裂纺锤杆处。这项工作强调了中心体中PCM蛋白的生物物理特性在细胞周期中波动。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhancement of CEP215 dynamics for spindle pole assembly during mitosis.

The microtubule-organizing activity of centrosomes fluctuates during the cell cycle, reaching the highest levels at M phase. CEP215 (also known as CDK5RAP2) is a key pericentriolar material (PCM) protein for microtubule organization of the human centrosome. Here, we provide evidence that CEP215 exhibits a dynamically suppressed, solid-like state in interphase centrosomes, and becomes a more dynamic state in mitotic centrosomes. Specific interaction with PCNT, another centrosome protein, is crucial for diffusible molecular dynamicity of the CEP215 protein. We also found that the cluster formation activity of CEP215 is impaired in a light-inducible system when its coiled-coil domains (CCDs) are truncated. Defects in spindle pole assembly and spindle formation were accompanied in the cells whose CEP215 is replaced with the CCD-truncated mutants. Our results support the notion that the diffusible mobility of CEP215 is enhanced by both homotypic and heterotypic interactions among CCDs, especially at mitotic spindle poles. This work highlights that biophysical properties of the PCM proteins at the centrosomes fluctuate during the cell cycle.

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来源期刊
Journal of cell science
Journal of cell science 生物-细胞生物学
CiteScore
7.30
自引率
2.50%
发文量
393
审稿时长
1.4 months
期刊介绍: Journal of Cell Science publishes cutting-edge science, encompassing all aspects of cell biology.
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