微细巴西棕榈蜡配制的分生绿僵菌对埃及伊蚊成虫自我梳理行为的影响。

IF 1.6 3区 农林科学 Q2 ENTOMOLOGY
Lucas Santos, Juscelino Rodrigues, Christian Luz
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引用次数: 0

摘要

埃及伊蚊(双翅目,库蚊科)是热带地区人类病毒性疾病的主要媒介,易被昆虫病原真菌感染。蚊子的自我梳理行为在很大程度上尚未得到研究,它可能会通过角质层干扰真菌感染。在这项研究中,埃及伊蚊成虫暴露于黄绿僵菌Luz, Rocha和Delalibera 2019 (Hypocreales, Clavicipitaceae)的分生孢子(补充或不补充微粉巴西棕榈蜡(MCW))后,在暴露于真菌处理过的表面24小时内,其体表未发芽的分生孢子和早期萌发阶段减少。暴露于加、不加或只加MCW的分生孢子后,成虫的自我梳理行为明显表现出低水平、自发的、不依赖刺激的自我梳理行为。第一对腿同时刮着鼻子、触角和翅膀;在三个单独的动作中,第三对腿(a)相互刮擦,(b)第一和第二腿,(c)性腺。暴露后,在跗骨、胫骨和股骨(特别是第一和第二腿对)、性腺、喙、触角和翅膀上很容易检测到分生孢子、分生孢子/MCW混合物或MCW(不含真菌)。在活动的成虫中,暴露后24小时内,平均分生孢子数下降了大约5倍,但在前6小时内,无论分生孢子准备情况如何,平均分生孢子数下降的幅度较小,然后在剩余的18小时内下降的幅度较小。对于冷固定的成虫,在暴露后的第一天,分生孢子的损失明显较低或不显著。在试验期间,MCW既没有增加表皮上分生孢子的数量,也没有延长表皮上分生孢子的停留时间。这些发现加强了在开发用于生物防治的特定真菌制剂时考虑蚊子自我梳理的重要性——特别是关于本文研究的媒介埃及伊蚊。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Metarhizium humberi conidia formulated with micronized carnauba wax on self-grooming behaviours by Aedes aegypti adults.

Aedes aegypti L. (Diptera, Culicidae), the key vector of viral diseases to humans in the tropics, is susceptible to infection by entomopathogenic fungi. Self-grooming behaviour, which remains largely unstudied for mosquitoes, may possibly interfere with fungal infection through the cuticle. In this study, self-grooming by A. aegypti adults exposed to Metarhizium humberi Luz, Rocha & Delalibera 2019 (Hypocreales, Clavicipitaceae) conidia supplemented or not with micronized carnauba wax (MCW) led to a decrease of non-germinated conidia and of early germinative stages from their body surface up to 24 h after exposure to a fungus-treated surface. Self-grooming behaviours by adults distinctly showed increased low-level, spontaneous and stimulus-independent self-grooming after exposure to conidia with or without added MCW or only to MCW. The first pair of legs simultaneously scraped the proboscis, antennae and wings; in three separate actions, the third pair of legs scraped (a) each other, (b) the first and second legs and (c) the gonapophyses. Immediately after exposure, conidia, a conidial/MCW mixture or MCW (without fungus) was readily detectable on tarsi, tibias and femurs (especially of the first and second leg pairs), gonapophyses, proboscis, antennae and wings. In mobile adults, mean conidial numbers dropped approximately fivefold during the 24 h immediately after exposure, but especially during the first 6 h, regardless of the conidial preparation and then, to a lesser extent, in the remaining 18 h. For cold-immobilized adults, during the first day post-exposure, conidial losses were distinctly lower or insignificant. MCW neither increased the number nor enhanced the retention time of conidia on the cuticle during the time tested. These findings strengthen the importance of considering self-grooming of mosquitoes - particularly regarding A. aegypti, the vector examined here - when developing specific fungal formulations for use in biological control.

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来源期刊
Medical and Veterinary Entomology
Medical and Veterinary Entomology 农林科学-昆虫学
CiteScore
3.70
自引率
5.30%
发文量
65
审稿时长
12-24 weeks
期刊介绍: Medical and Veterinary Entomology is the leading periodical in its field. The Journal covers the biology and control of insects, ticks, mites and other arthropods of medical and veterinary importance. The main strengths of the Journal lie in the fields of: -epidemiology and transmission of vector-borne pathogens changes in vector distribution that have impact on the pathogen transmission- arthropod behaviour and ecology- novel, field evaluated, approaches to biological and chemical control methods- host arthropod interactions. Please note that we do not consider submissions in forensic entomology.
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