沃顿氏胶状间充质干细胞向功能性颗粒细胞分化潜能的性别差异。

IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Sang-Yun Lee, Dinesh Bharti, Young-Bum Son, Won-Jae Lee, Yong-Ho Choe, Hyeon-Jeong Lee, Seong-Ju Oh, Tae-Seok Kim, Chae-Yeon Hong, Sung-Lim Lee, Gyu-Jin Rho
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引用次数: 0

摘要

卵泡由卵泡膜细胞和颗粒细胞组成,它们在性激素和细胞因子的分泌中起着至关重要的生理作用,并为卵母细胞提供了最佳的诱导微环境。然而,伦理问题和缺乏用于研究人类分子途径的细胞模型为颗粒细胞的研究带来了挑战。为了解决这些挑战,利用间充质干细胞(MSCs)诱导颗粒细胞分化可能为推进颗粒细胞研究提供一种新的途径。本研究研究了来自男性和女性供体的间充质干细胞的颗粒细胞分化能力和激素合成功能,以确定性别差异。从Wharton’s jelly中分离的MSCs (WJ-MSCs)成功分化为颗粒细胞样细胞,证实了颗粒细胞特异性标记在mRNA和蛋白水平上的表达。分化为颗粒状细胞样细胞的WJ-MSCs增加了芳香酶活性,该酶在将睾酮转化为雌二醇的过程中起重要作用,导致分化细胞中雌二醇水平明显高于未分化的WJ-MSCs。然而,雌性分化细胞的活性明显高于雄性分化细胞。目前的研究表明,女性来源的WJ-MSCs可能为理解颗粒细胞提供了一种新的干细胞资源,并为研究人类卵泡发生的各个发育阶段和过程提供了良好的细胞来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gender difference of Wharton's jelly-derived mesenchymal stem cells on differentiation potential into functional granulosa cells.

The ovarian follicles consist of theca and granulosa cells, which play a crucial physiological role in sex hormone and cytokine secretion and provide an optimal induction microenvironment for oocytes. However, ethical concerns and the absence of a cellular model for investigating the molecular pathway in humans present challenges for research on granulosa cells. To address these challenges, differentiation induction into granulosa cells using mesenchymal stem cells (MSCs) could offer a novel approach to advancing granulosa cell research. In this study, the granulosa cell differentiation ability and hormone synthesis function of MSCs derived from male and female donors were investigated to identify gender differences. MSCs isolated from Wharton's jelly (WJ-MSCs) were successfully differentiated into granulosa cell-like cells, as evidenced by the expression of granulosa cell-specific markers at both the mRNA and protein levels. Differentiated WJ-MSCs into granulosa cell-like cells increased aromatase activity, which plays an important role in converting testosterone to estradiol, resulting in significantly increased estradiol levels in differentiated cells compared to undifferentiated WJ-MSCs. However, the activity in female-differentiated cells was significantly higher than in male-differentiated cells. The current study indicates that female-derived WJ-MSCs may represent a novel stem cell resource for understanding granulosa cells and could provide an excellent cellular source for studying various developmental stages and processes of human folliculogenesis.

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来源期刊
Cellular and molecular biology
Cellular and molecular biology 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
331
期刊介绍: Cellular and Molecular Biology publishes original articles, reviews, short communications, methods, meta-analysis notes, letters to editor and comments in the interdisciplinary science of Cellular and Molecular Biology linking and integrating molecular biology, biophysics, biochemistry, enzymology, physiology and biotechnology in a dynamic cell and tissue biology environment, applied to human, animals, plants tissues as well to microbial and viral cells. The journal Cellular and Molecular Biology is therefore open to intense interdisciplinary exchanges in medical, dental, veterinary, pharmacological, botanical and biological researches for the demonstration of these multiple links.
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