空气-液界面急性暴露于乙苯和间二甲苯及二元混合物后BEAS-2B细胞的毒理学反应

IF 2.8 4区 医学 Q3 TOXICOLOGY
Nour Jaber, Claude Emond, Fabrice Cazier, Sylvain Billet
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引用次数: 0

摘要

苯、甲苯、乙苯和二甲苯(邻二甲苯、间二甲苯和对二甲苯)构成了一个名为BTEX的家族,它是挥发性有机化合物(VOCs),以其毒性而闻名。本研究旨在研究乙苯和间二甲苯对暴露于气液界面(ALI)的人支气管上皮细胞的急性体外毒性。将细胞单独暴露于VOCs和混合暴露于VOCs中1小时,然后分别孵育5、23和47小时。表征了细胞反应的动力学,包括细胞毒性、异种生物转化、抗氧化防御系统、炎症反应和凋亡。尽管这两种化合物的化学结构非常相似,但基因表达结果显示它们之间存在重大差异。乙苯在BEAS-2B细胞中似乎没有代谢,因为它抑制了外源代谢酶(XME)的基因表达,并且不会诱导抗氧化防御系统或细胞凋亡。然而,暴露后观察到轻微的炎症反应。m-二甲苯在BEAS-2B细胞中代谢,诱导多种参与抗氧化防御系统的xme和上调酶,以及炎症和凋亡标志物。共同暴露于二元混合物中会产生抑制现象,从而对抑制毒性作用机制进行了研究。这些结果提供了关于乙苯和间二甲苯毒性的新信息,并强调了将ALI暴露于毒物混合物中的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Toxicological Response of the BEAS-2B Cell After Acute Exposure at the Air-Liquid Interface to Ethylbenzene and m-Xylene Alone and in Binary Mixtures.

Benzene, toluene, ethylbenzene, and xylenes (o-, m-, and p-xylenes) constitute a family, named BTEX, of volatile organic compounds (VOCs) known for its toxicity. This study aimed to study the acute in vitro toxicity of ethylbenzene and m-xylene on human bronchial epithelial cells exposed at the air-liquid interface (ALI). The cells were exposed to VOCs alone and in a mixture for 1 h, followed by 5, 23, and 47 h of incubation. The kinetics of the cell response was characterized, including cytotoxicity, xenobiotic biotransformation, antioxidant defense system, inflammatory response, and apoptosis. The gene expression results showed major differences between these two compounds, even though their chemical structure is very similar. Ethylbenzene did not appear to be metabolized in BEAS-2B cells, as it inhibited gene expression of xenobiotic metabolizing enzymes (XME) and did not induce antioxidant defense systems or apoptosis. However, a slight inflammatory response was observed after exposure. m-Xylene was metabolized in BEAS-2B cells, inducing several XMEs and upregulating enzymes involved in the antioxidant defense system, as well as markers of inflammation and apoptosis. Co-exposure to the binary mixture resulted in an inhibition phenomenon, resulting in the inhibition of toxic action mechanisms studied. The results provide new information on the toxicity of ethylbenzene and m-xylene and highlight the importance of conducting ALI exposures to mixtures of toxicants.

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来源期刊
CiteScore
7.00
自引率
6.10%
发文量
145
审稿时长
1 months
期刊介绍: Journal of Applied Toxicology publishes peer-reviewed original reviews and hypothesis-driven research articles on mechanistic, fundamental and applied research relating to the toxicity of drugs and chemicals at the molecular, cellular, tissue, target organ and whole body level in vivo (by all relevant routes of exposure) and in vitro / ex vivo. All aspects of toxicology are covered (including but not limited to nanotoxicology, genomics and proteomics, teratogenesis, carcinogenesis, mutagenesis, reproductive and endocrine toxicology, toxicopathology, target organ toxicity, systems toxicity (eg immunotoxicity), neurobehavioral toxicology, mechanistic studies, biochemical and molecular toxicology, novel biomarkers, pharmacokinetics/PBPK, risk assessment and environmental health studies) and emphasis is given to papers of clear application to human health, and/or advance mechanistic understanding and/or provide significant contributions and impact to their field.
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