{"title":"尼日利亚动物园圈养野生鸟类产esbl大肠杆菌的分子和流行病学特征","authors":"Ridwan Olamilekan Adesola, Adetolase Azizat Bakre, Abimbola Olumide Adekanmbi, Bamidele Nyemike Ogunro, Oluwaseun Adeolu Ogundijo, Abdulafees Hamzat, Delower Hossain, Moses Aimanosi Aribana, Luqman Adeola Balogun","doi":"10.1177/11786302251329300","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>This study aimed to characterize ESBL-producing <i>E. coli</i> isolates obtained from apparently healthy captive wild birds from selected zoological gardens in Nigeria.</p><p><strong>Materials and methods: </strong>A total of 121 freshly deposited faecal samples were collected from captive wild birds in seven major zoological gardens and pooled into 50 samples. The samples were screened for the presence of <i>E. coli</i>. The isolates obtained were tested against a panel of antibiotics and screened for ESBL production using the double disc synergy test (DDST). Primer-specific PCR was used to detect the carriage of ESBL genes (<i>bla</i> <sub>CTX-M</sub>, <i>bla</i> <sub>TEM</sub> and <i>bla</i> <sub>SHV</sub>) by the isolates.</p><p><strong>Results: </strong>A total of 26 ESBL-producing <i>E. coli</i> isolates (52%, n = 26/50) were obtained from the pooled faecal samples of captive wild birds. The highest resistance rate to antibiotics was observed with amoxicillin-clavulanate (88.5%), while the lowest resistance rate was observed with fosfomycin (3.8%). The isolates had Multiple Antibiotic Resistance Index (MARI) values ranging from 0.1 to 0.8 across the tested antibiotics. Approximately 65.4% of the isolates carried <i>bla</i> <sub>CTX-M</sub>, while <i>bla</i> <sub>TEM</sub> and <i>bla</i> <sub>SHV</sub> were detected in 15.4% and 34.6% of the isolates, respectively.</p><p><strong>Conclusion: </strong>This study revealed a significant prevalence of ESBL-producing <i>E. coli</i> in captive wild birds in Nigeria and highlighted the need to institute control measures in zoological gardens to prevent the transmission of antibiotic resistance.</p>","PeriodicalId":11827,"journal":{"name":"Environmental Health Insights","volume":"19 ","pages":"11786302251329300"},"PeriodicalIF":2.3000,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12035045/pdf/","citationCount":"0","resultStr":"{\"title\":\"Molecular and Epidemiological Characterization of ESBL-producing <i>Escherichia coli</i> from Captive Wild Birds in Zoological Gardens in Nigeria.\",\"authors\":\"Ridwan Olamilekan Adesola, Adetolase Azizat Bakre, Abimbola Olumide Adekanmbi, Bamidele Nyemike Ogunro, Oluwaseun Adeolu Ogundijo, Abdulafees Hamzat, Delower Hossain, Moses Aimanosi Aribana, Luqman Adeola Balogun\",\"doi\":\"10.1177/11786302251329300\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim: </strong>This study aimed to characterize ESBL-producing <i>E. coli</i> isolates obtained from apparently healthy captive wild birds from selected zoological gardens in Nigeria.</p><p><strong>Materials and methods: </strong>A total of 121 freshly deposited faecal samples were collected from captive wild birds in seven major zoological gardens and pooled into 50 samples. The samples were screened for the presence of <i>E. coli</i>. The isolates obtained were tested against a panel of antibiotics and screened for ESBL production using the double disc synergy test (DDST). Primer-specific PCR was used to detect the carriage of ESBL genes (<i>bla</i> <sub>CTX-M</sub>, <i>bla</i> <sub>TEM</sub> and <i>bla</i> <sub>SHV</sub>) by the isolates.</p><p><strong>Results: </strong>A total of 26 ESBL-producing <i>E. coli</i> isolates (52%, n = 26/50) were obtained from the pooled faecal samples of captive wild birds. The highest resistance rate to antibiotics was observed with amoxicillin-clavulanate (88.5%), while the lowest resistance rate was observed with fosfomycin (3.8%). The isolates had Multiple Antibiotic Resistance Index (MARI) values ranging from 0.1 to 0.8 across the tested antibiotics. Approximately 65.4% of the isolates carried <i>bla</i> <sub>CTX-M</sub>, while <i>bla</i> <sub>TEM</sub> and <i>bla</i> <sub>SHV</sub> were detected in 15.4% and 34.6% of the isolates, respectively.</p><p><strong>Conclusion: </strong>This study revealed a significant prevalence of ESBL-producing <i>E. coli</i> in captive wild birds in Nigeria and highlighted the need to institute control measures in zoological gardens to prevent the transmission of antibiotic resistance.</p>\",\"PeriodicalId\":11827,\"journal\":{\"name\":\"Environmental Health Insights\",\"volume\":\"19 \",\"pages\":\"11786302251329300\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-04-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12035045/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Health Insights\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1177/11786302251329300\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Health Insights","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/11786302251329300","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH","Score":null,"Total":0}
Molecular and Epidemiological Characterization of ESBL-producing Escherichia coli from Captive Wild Birds in Zoological Gardens in Nigeria.
Aim: This study aimed to characterize ESBL-producing E. coli isolates obtained from apparently healthy captive wild birds from selected zoological gardens in Nigeria.
Materials and methods: A total of 121 freshly deposited faecal samples were collected from captive wild birds in seven major zoological gardens and pooled into 50 samples. The samples were screened for the presence of E. coli. The isolates obtained were tested against a panel of antibiotics and screened for ESBL production using the double disc synergy test (DDST). Primer-specific PCR was used to detect the carriage of ESBL genes (blaCTX-M, blaTEM and blaSHV) by the isolates.
Results: A total of 26 ESBL-producing E. coli isolates (52%, n = 26/50) were obtained from the pooled faecal samples of captive wild birds. The highest resistance rate to antibiotics was observed with amoxicillin-clavulanate (88.5%), while the lowest resistance rate was observed with fosfomycin (3.8%). The isolates had Multiple Antibiotic Resistance Index (MARI) values ranging from 0.1 to 0.8 across the tested antibiotics. Approximately 65.4% of the isolates carried blaCTX-M, while blaTEM and blaSHV were detected in 15.4% and 34.6% of the isolates, respectively.
Conclusion: This study revealed a significant prevalence of ESBL-producing E. coli in captive wild birds in Nigeria and highlighted the need to institute control measures in zoological gardens to prevent the transmission of antibiotic resistance.
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