黄芪多糖减轻d -半乳糖诱导衰老的认知能力下降。

IF 1.7 4区 医学 Q3 PHARMACOLOGY & PHARMACY
Jin Tian, Ran Huo, Yixuan Wang, Jiepeng Wang, Fang Fang, Chaoyi Fang
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引用次数: 0

摘要

黄芪多糖(黄芪多糖)是从黄芪茎或根中提取的具有生物活性的水溶性多糖,已被证明具有抗衰老作用。本研究旨在探讨黄芪多糖(APS)对d-半乳糖(d-gal)诱导衰老大鼠认知功能的影响,并探讨其可能的分子机制。以400 mg/kg/d -gal腹腔注射诱导衰老,持续8周。采用Morris水迷宫实验、降压实验、空地实验和握力实验评估大鼠的衰老情况。苏木精染色、伊红染色、尼氏染色检测海马CA3、CA1区的病理变化。测定血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)和总抗氧化能力(T-AOC)。检测端粒长度、双氧化酶1 (Duox1)、双氧化酶2 (Duox2)、过氧化物还蛋白1 (Prdx1)、p21、p16、p53、端粒酶逆转录酶(TERT)、磷脂酰肌醇3-激酶(PI3K)、蛋白激酶B (AKT)、烟酰胺磷酸核糖基转移酶(NAMPT)、sirtuin 1 (SIRT1)。结果表明,黄芪多糖改善了d-gal诱导的衰老大鼠的一般状态,减轻了CA3和CA1区域的神经元变性,降低了氧化应激水平,调节了衰老相关β-GAL和蛋白的表达,并维持了端粒长度。此外,APS显著降低p53表达,增加p-PI3K、p-AKT、NAMPT、SIRT1和TERT表达。因此,APS可能通过PI3K/Akt和NAMPT/SIRT1信号通路调控TERT/p53信号轴,从而预防d-gal诱导的大鼠衰老和认知功能障碍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Astragalus Polysaccharide Alleviates Cognitive Decline in D-Galactose-Induced Aging.

Astragalus polysaccharide (APS) is a biologically active water-soluble polysaccharide extracted from stems or roots, which has been proven to have antiaging effects. The aim of this study was to investigate the effects of APS on cognitive function in d-galactose (d-gal)-induced aging rats and explore the potential underlying molecular mechanisms. The rats were induced to age by intraperitoneal injection with 400 mg/kg/d d-gal for 8 weeks. Aging of rats was assessed through the Morris water maze test, step-down test, open field test, and grip strength test. Pathological changes in the hippocampal CA3 and CA1 regions were determined by Hematoxylin and eosin and Nissl staining. The superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), and total antioxidant capacity (T-AOC) in the serum were measured. Telomere length, dual oxidase 1 (Duox1), dual oxidase 2 (Duox2), peroxiredoxin 1 (Prdx1), p21, p16, p53, telomerase reverse transcriptase (TERT), phosphatidylinositol 3-kinase (PI3K), protein kinase B (AKT), nicotinamide phosphoribosyl transferase (NAMPT), and sirtuin 1 (SIRT1) were detected via real-time PCR, Western blotting, and immunohistochemical staining. The results indicated that APS ameliorated the general status in d-gal-induced aging rats, mitigated neuronal degeneration in the CA3 and CA1 regions, reduced the oxidative stress levels, modulated senescence-related β-GAL and protein expression, and maintained telomere length. Furthermore, APS significantly reduced p53 expression and increased p-PI3K, p-AKT, NAMPT, SIRT1, and TERT expression. Therefore, d-gal-induced aging and cognitive impairment in rats can be prevented by APS, likely through regulation of the TERT/p53 signaling axis via the PI3K/Akt and NAMPT/SIRT1 signaling pathways.

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来源期刊
CiteScore
3.50
自引率
5.00%
发文量
247
审稿时长
2 months
期刊介绍: Biological and Pharmaceutical Bulletin (Biol. Pharm. Bull.) began publication in 1978 as the Journal of Pharmacobio-Dynamics. It covers various biological topics in the pharmaceutical and health sciences. A fourth Society journal, the Journal of Health Science, was merged with Biol. Pharm. Bull. in 2012. The main aim of the Society’s journals is to advance the pharmaceutical sciences with research reports, information exchange, and high-quality discussion. The average review time for articles submitted to the journals is around one month for first decision. The complete texts of all of the Society’s journals can be freely accessed through J-STAGE. The Society’s editorial committee hopes that the content of its journals will be useful to your research, and also invites you to submit your own work to the journals.
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