济州黑牛朊样蛋白Doppel (PRND)基因多态性检测。

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Genome Pub Date : 2025-01-01 DOI:10.1139/gen-2024-0145
Thi-Thuy-Duong Nguyen, Da-In Choi, Kyoungtag Do, Nameun Kim, Byung-Hoon Jeong
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引用次数: 0

摘要

朊蛋白(PrP)在牛海绵状脑病(BSE)中起着核心作用。鉴于其与PrP的结构和生物学相似性,近年来的研究重点是由朊病毒样蛋白Doppel (PRND)基因编码的Doppel (Dpl)蛋白。本研究对100头韩国本土品种济州黑牛的PRND基因多态性进行了分析。我们使用SIFT、PANTHER和polyphen2三种计算机工具预测了非同义单核苷酸多态性(SNPs)对Dpl蛋白功能的潜在影响。最后,我们比较分析了c.395A >g (Q132R)在德国疯牛病感染牛和韩国健康牛之间的基因型分布,以评估韩国牛对疯牛病的易感性。我们发现了8个snp,包括3个新的snp c - 7c >T, c.172G>A (A58T)和c.537+83C>T。SIFT预测只有c.172G>A会产生有害影响。对比分析,荷斯坦牛和济州黑牛患疯牛病的潜在风险较高。据我们所知,这是济州黑牛PRND基因遗传特征的第一份报告。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection of polymorphisms in the prion-like protein Doppel (PRND) gene in Jeju black cattle.

Prion protein (PrP) plays a central role in bovine spongiform encephalopathy (BSE). Given the structural and biological similarity to PrP, recent research has focused on Doppel (Dpl) protein, which is encoded by the prion-like protein Doppel (PRND) gene. In this study, we characterized the PRND genetic polymorphism in 100 Jeju black cattle, a native Korean breed. We predicted the potential effect of nonsynonymous single nucleotide polymorphisms (SNPs) on the Dpl protein function using three in silico tools: SIFT, PANTHER, and PolyPhen-2. Finally, we performed a comparative analysis of the genotype distribution of c.395A>G (Q132R) between German BSE-infected cattle and Korean healthy cattle to assess the BSE susceptibility of Korean cattle. We found eight SNPs, including three novel SNPs c.-7C>T, c.172G>A (A58T), and c.537+83C>T. Only c.172G>A was predicted by SIFT to exert a deleterious effect. According to the comparative analysis, Holstein and Jeju black cattle exhibited a higher potential risk for BSE. To the best of our knowledge, this is the first report on the genetic characteristics of the PRND gene in Jeju black cattle.

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来源期刊
Genome
Genome 生物-生物工程与应用微生物
CiteScore
5.30
自引率
3.20%
发文量
42
审稿时长
6-12 weeks
期刊介绍: Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.
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