熊去氧胆酸改善紫杉醇诱导的大鼠肝损伤:氧化和DNA损伤、细胞凋亡和自噬的评估。

IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES
Mehmet Eki Ci, Mustafa Özkaraca, Hilmi Ataseven
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引用次数: 0

摘要

紫杉醇(Paclitaxel, PTX)是一种广泛应用于化疗的药物,具有严重的肝毒性等副作用。本研究旨在探讨熊去氧胆酸(UDCA)对ptx诱导的肝损伤的治疗作用。熊去氧胆酸是一种二级胆汁酸,是肠道细菌的副产物,具有强大的抗氧化特性。PTX以2 mg*kg-1腹腔注射,连续5天诱导肝损伤。第一组大鼠口服UDCA,剂量为60 mg*kg-1,连续15 d;第二组大鼠口服相同剂量,连续10 d。使用自动分析仪分析血清AST、ALT、ALP、LDH、总蛋白和白蛋白水平。评估肝组织总氧化状态(TOS)、氧化应激指数(OSI)和总抗氧化状态(TAS)。同时进行组织病理学和免疫组织化学分析。UDCA可以改善ptx诱导的体重和肝酶的变化。减少肝组织坏死、水变性和单核细胞浸润。UDCA还能显著降低TOS和OSI水平,减轻氧化应激,增加TAS。此外,UDCA通过降低8-OHdG免疫反应性来减轻DNA损伤,并通过逆转升高的LC3B和裂解的caspase-3水平来调节自噬和凋亡。这些发现表明,UDCA可能对ptx诱导的肝毒性有治疗作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ursodeoxycholic acid ameliorates paclitaxel-induced hepatic injury in rats: Evaluation of oxidative and DNA damage, apoptosis and autophagy.

Paclitaxel (PTX), widely used in chemotherapy, can cause serious side effects such as hepatotoxicity. This study aimed to investigate the therapeutic effects of ursodeoxycholic acid (UDCA) - a secondary bile acid, a byproduct of intestinal bacteria with potent antioxidant properties - against PTX-induced liver injury. PTX was administered intraperitoneally at 2 mg*kg-1 for 5 days to induce liver damage. Rats in the first treatment group received UDCA orally at 60 mg*kg-1 for fifteen days, while the second group received the same dose for ten days. Serum levels of AST, ALT, ALP, LDH, total protein, and albumin were analysed using an autoanalyzer. Liver tissue was assessed for total oxidative status (TOS), oxidative stress index (OSI) and total antioxidant status (TAS). Histopathological and immunohistochemical analyses were also conducted. UDCA administration ameliorated PTX-induced changes in bodyweight and liver enzymes. It reduced necrosis, hydropic degeneration and mononuclear cell infiltration in liver tissue. UDCA also significantly decreased TOS and OSI levels, alleviating oxidative stress, and increased TAS. Furthermore, UDCA reduced DNA damage, evidenced by lower 8-OHdG immunoreactivity, and regulated autophagy and apoptosis by reversing elevated LC3B and cleaved caspase-3 levels. These findings suggest that UDCA may offer therapeutic benefits against PTX-induced hepatotoxicity.

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来源期刊
Acta veterinaria Hungarica
Acta veterinaria Hungarica 农林科学-兽医学
CiteScore
1.80
自引率
0.00%
发文量
39
审稿时长
>36 weeks
期刊介绍: Acta Veterinaria Hungarica publishes original research papers presenting new scientific results of international interest, and to a limited extent also review articles and clinical case reports, on veterinary physiology (physiological chemistry and metabolism), veterinary microbiology (bacteriology, virology, immunology, molecular biology), on the infectious diseases of domestic animals, on veterinary parasitology, pathology, clinical veterinary science and reproduction.
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