Luca Musella, Jens Möller, Christopher Lischer, Julio Vera-Gonzalez, Jörg Hofmann, Lisa Ott, Andreas Burkovski
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Proteome Analysis of Corynebacterium diphtheriae-Macrophage Interaction.
Contact of Corynebacterium diphtheriae with macrophages induces adaptations on both bacterial and cellular sides. The study presented here was aiming to shed light on the simultaneous intracellular adaptation of the bacteria and changes in the proteome of the phagocytes in response to the internalization of C. diphtheriae. Quantitative proteome analyses were carried out at different time points of an infection assay and data were analyzed by different bioinformatic approaches. Several C. diphtheriae proteins, which were not observed or connected with pathogenicity before, were differentially expressed, as well as key macrophage components of the phagolysosome. Overall, bacteria responded to phagocytosis by changes in DNA repair, transcription, and cell wall synthesis proteins, while macrophages showed changes in components of the innate immune system.
期刊介绍:
PROTEOMICS is the premier international source for information on all aspects of applications and technologies, including software, in proteomics and other "omics". The journal includes but is not limited to proteomics, genomics, transcriptomics, metabolomics and lipidomics, and systems biology approaches. Papers describing novel applications of proteomics and integration of multi-omics data and approaches are especially welcome.