血小板活化因子(PAF)诱导大鼠食管和胃底平滑肌强机械活动并伴有基底张力升高。

IF 1.7 4区 医学 Q3 PHARMACOLOGY & PHARMACY
Keisuke Obara, Sana Takahashi, Miho Otake, Mako Fujiwara, Mio Yamashita, Azusa Murata, Kento Yoshioka, Yoshio Tanaka
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引用次数: 0

摘要

据报道,在大鼠中,血小板活化因子(PAF)可增加除食道平滑肌外的各种胃肠道平滑肌(SMs)的机械活动。本研究的目的是研究PAF是否会增加大鼠食管纵向SM (LSM)的机械活动,并比较PAF在食管纵向SM和其他胃肠道LSM中的作用。PAF (10-9-10-6 M)以浓度依赖性方式增加食管LSM的机械活动;PAF主要引起基底张力升高,几乎被PAF受体拮抗剂CV-6209 (10-5 M;10-6 M PAF)。胃底LSM与食道LSM相似,在发育过程中起源于前肠,PAF (10-6 M)将基底张力增加到与食道LSM相当的程度,尽管明显不同。相比之下,来自中肠的十二指肠-空肠、回肠和升结肠以及来自后肠的降结肠的lsm中,PAF (10-6 M)增加基础张力的能力低于食管和胃底lsm。有趣的是,在升结肠lsm中,PAF (10-6 M)诱导振荡性收缩,同时基底张力小幅增加。paf诱导的收缩与paf降解酶Pafah2 (R = 0.82)和Pafah1b3 (R = 0.51) mRNA表达量呈正相关。这些结果表明,PAF强烈刺激大鼠胚胎发生期间源自前肠的胃肠道lsm的机械活动,主要伴随着基础张力的增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Platelet-Activating Factor (PAF) Induces Strong Mechanical Activities Accompanied by Basal Tension Increases in Esophageal and Gastric Fundus Smooth Muscles from Rat.

In rats, platelet-activating factor (PAF) has been reported to increase mechanical activity in various gastrointestinal smooth muscles (SMs) except for esophagus SM. The aim of this study was to examine whether PAF increases mechanical activity in rat esophagus longitudinal SM (LSM) and to compare PAF actions in esophagus LSM with those in other gastrointestinal LSMs. PAF (10-9-10-6 M) increased esophagus LSM mechanical activities in a concentration-dependent manner; PAF mainly elicited basal tension increases that were almost eliminated by a PAF receptor antagonist CV-6209 (10-5 M; against 10-6 M PAF). In the LSM of the gastric fundus, which is similar to esophagus LSM in that it is derived from the foregut during development, PAF (10-6 M) increased basal tension to a comparable, albeit significantly different, magnitude as in esophagus LSM. In contrast, in LSMs of the duodenum-jejunum, ileum, and ascending colon, which are derived from the midgut, and the descending colon, which is derived from the hindgut, the ability of PAF (10-6 M) to increase basal tension was less than that in esophagus and gastric fundus LSMs. Interestingly, in ascending colon LSMs, PAF (10-6 M) induced oscillatory contractions with a small increase in basal tension. PAF-induced contractions were positively correlated with the mRNA expression levels of the PAF-degrading enzymes Pafah2 (R = 0.82) and Pafah1b3 (R = 0.51). These results suggest that PAF strongly stimulates mechanical activities that are mainly accompanied by basal tension increases in rat LSMs of the gastrointestinal tracts that are derived from the foregut during embryogenesis.

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来源期刊
CiteScore
3.50
自引率
5.00%
发文量
247
审稿时长
2 months
期刊介绍: Biological and Pharmaceutical Bulletin (Biol. Pharm. Bull.) began publication in 1978 as the Journal of Pharmacobio-Dynamics. It covers various biological topics in the pharmaceutical and health sciences. A fourth Society journal, the Journal of Health Science, was merged with Biol. Pharm. Bull. in 2012. The main aim of the Society’s journals is to advance the pharmaceutical sciences with research reports, information exchange, and high-quality discussion. The average review time for articles submitted to the journals is around one month for first decision. The complete texts of all of the Society’s journals can be freely accessed through J-STAGE. The Society’s editorial committee hopes that the content of its journals will be useful to your research, and also invites you to submit your own work to the journals.
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