半乳糖-α-1,3-半乳糖呈递细菌家族与实验性禽疟疾感染的抗性有关

IF 1.8 3区 生物学 Q1 ORNITHOLOGY
R. K. Noble, T. R. Kelly, C. R. Lattin
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引用次数: 0

摘要

肠道微生物群可以通过防御病原体来促进宿主的健康。然而,宿主肠道微生物群与感染抗性之间的联系主要在人类和实验室饲养的啮齿动物中进行了研究,因此我们不知道这种关系在野生动物中存在到何种程度。为了填补这一知识空白,我们在野生捕获的家雀Passer domesticus (n = 18)中接种了实验性的直肠疟原虫疟疾疫苗,以研究宿主肠道微生物群的相互作用和对禽疟疾感染的抗性。接种前后采集血液样本定量循环寄生虫血症,采集粪便样本通过细菌16S rRNA基因V4区扩增和测序评估肠道微生物组。我们发现,在疟疾感染前或感染期间,感染和抗性麻雀之间的肠道微生物组α多样性(物种丰富度和均匀度)和β多样性(群落组成)没有显著差异。然而,我们发现了几种指示性asv,它们在疟疾抗性麻雀中以较高的患病率和比例丰度出现,其中大多数来自乳酸杆菌科,据推测,乳酸杆菌科通过诱导抗α- gal抗体的产生而赋予疟疾抗性。未来的研究应通过实验操作禽肠道微生物组的组成,以因果关系研究宿主肠道微生物组中特定细菌类群与禽疟疾感染抗性之间的关系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Galactose-α-1,3-galactose-presenting bacterial families are associated with resistance to experimental avian malaria infection

Galactose-α-1,3-galactose-presenting bacterial families are associated with resistance to experimental avian malaria infection

Galactose-α-1,3-galactose-presenting bacterial families are associated with resistance to experimental avian malaria infection

Galactose-α-1,3-galactose-presenting bacterial families are associated with resistance to experimental avian malaria infection

The gut microbiome can contribute to host health through defense against pathogens. However, links between the host gut microbiome and resistance to infection have been primarily investigated in humans and lab-bred rodents, so we do not know to what extent this relationship exists in wild animals. To fill this knowledge gap, we used an experimental malaria inoculation with Plasmodium relictum in wild-caught house sparrows Passer domesticus (n = 18) to examine interactions of the host gut microbiome and resistance to avian malaria infection. Before and after inoculation, we collected blood samples to quantify circulating parasitemia and fecal samples to evaluate the gut microbiome through amplification and sequencing of the V4 region of the bacterial 16S rRNA gene. We found that gut microbiome alpha diversity (species richness and evenness) and beta diversity (community composition) were not significantly different between infected and resistant sparrows prior to or during malaria infection. However, we identified several indicator ASVs that occurred at higher prevalence and proportional abundance in malaria-resistant sparrows, most of which were from the family Lactobacillaceae that is hypothesized to confer resistance to malaria through inducing the production of anti-α-Gal antibodies. Future studies should experimentally manipulate the composition of the avian gut microbiome to causally investigate the relationship between specific bacterial taxa in the host gut microbiome and resistance to avian malaria infection.

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来源期刊
Journal of Avian Biology
Journal of Avian Biology 生物-鸟类学
CiteScore
3.70
自引率
0.00%
发文量
56
审稿时长
3 months
期刊介绍: Journal of Avian Biology publishes empirical and theoretical research in all areas of ornithology, with an emphasis on behavioural ecology, evolution and conservation.
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