质子RBE的体外测量：使用统一设置的多中心比较

IF 2.7 3区医学 Q3 ONCOLOGY

Clinical and Translational Radiation Oncology Pub Date : 2025-05-11 DOI:10.1016/j.ctro.2025.100978

Olga Sokol , Anders Tobias Frederiksen , Mateusz Sitarz , Brita Singers Sørensen , Elham Santina , Christopher Smith , John-William Warmenhoven , Marie Davídková , Anna Jelínek Michaelidesová , Irina Danilová , Oldřich Zahradníček , Amélia Maia Leite , Ludovic de Marzi , Frederic Pouzoulet , Paweł Olko , Justyna Miszczyk , Barbara Orzechowska , Eleftherios Papalanis , Mehran Hariri , Diana Spiegelberg , Marco Durante

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引用次数: 0

摘要

背景和目的本研究对参与INSPIRE框架的六个欧洲机构在统一实验条件下进行的质子照射后的体外细胞存活测量和RBE计算进行了多中心比较。材料和方法sv79 -4细胞采用宽度为6 cm和4 cm的两种构型的铺展布拉格峰（SOBP）质子场辐照，分别输出6 Gy和8 Gy。每个中心都遵循标准化的方案，利用相同的模体设计来最小化与样本定位相关的不确定性。还进行了x射线参考照射，以评估参与中心的细胞放射敏感性。结果：尽管采用了一致的方案，但在生存测量中观察到显著的机构间差异。对于两种治疗方案，最大的变化是在SOBP的最远端点（6 Gy和8 Gy方案的变异系数分别为43%和60%）。Kruskal-Wallis统计检验证实了两种SOBP构型在质子场中每个测量位置的中心之间存在显著差异。在计算的RBE数据中也观察到差异，尽管保留了SOBP远端边缘值略有增加的预期趋势（在6 Gy和8 Gy计划中分别高达1.5和1.3）。结论本研究的结果突出了质子RBE测量在良好的实验条件下可以预期的最小生物学变异，以及进行大规模，多中心放射生物学实验和文献数据集之间的相互比较所面临的挑战。

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In vitro measurement of proton RBE: A multi-centric comparison using a harmonized setup

Background and purpose

This study presents a multi-center comparison of in vitro cell survival measurements and RBE calculations following proton irradiations conducted under harmonized experimental conditions across six European institutions participating in the INSPIRE framework.

Materials and methods

V79-4 cells were irradiated using spread-out Bragg peak (SOBP) proton fields of two configurations delivering 6 and 8 Gy with widths of 6 and 4 cm, respectively. Each center adhered to a standardized protocol, utilizing the same phantom design to minimize uncertainties related to sample positioning. X-ray reference irradiations were also performed to assess cell radiosensitivity across the participating centers.

Results

Despite the consistent protocol, significant inter-institutional variability was observed in the survival measurements. For both treatment plans, the largest variation was detected in the most distal points of the SOBP (coefficients of variation of 43 % and 60 % for the 6 Gy and 8 Gy plans, respectively). Kruskal-Wallis statistical test confirmed the significant differences between the centers for each of the measured position in the proton field for both SOBP configurations. Discrepancies were observed in calculated RBE data as well, albeit preserving the expected trend for the values to slightly increase towards the distal edge of the SOBP (up to 1.5 and 1.3 for the 6 Gy and 8 Gy plans, respectively).

Conclusion

The results of the study highlight the minimal biological variation one could expect performing proton RBE measurements in well-aligned experimental conditions and challenges in conducting large-scale, multi-center radiobiological experiments and inter-comparisons between literature data sets.

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