NF-κB signaling and its anti-apoptotic effects in liver & skeletal muscle of dehydrated Xenopus laevis

The African clawed frog, Xenopus laevis, is able to survive prolonged arid conditions during seasonal droughts. During these conditions, X. laevis enters aestivation whereby its metabolic rate is suppressed, urea and ammonia levels increase, and its physiological functions slow. Various molecular mechanisms are employed by X. laevis to mitigate the deleterious effects of severe dehydration and hypometabolism, including pro-survival cellular processes that protect cells and tissues from damage and atrophy. While previous research has focused on antioxidant proteins’ role in preventing oxidative stress, information on the role of anti-apoptotic signaling in X. laevis is lacking. As such, we investigated the role of nuclear factor-kappa B (NF-κB) signaling and its downstream target genes in liver and skeletal muscle tissue of X. laevis. The transcription factor, NF-κB, and its downstream target genes work to inhibit apoptotic machinery and promote cell survival. Herein, we found that NF-κB signaling activation in liver tissue leads to the selective upregulation of downstream anti-apoptotic proteins. In contrast, this upregulation occurs independently of NF-κB signaling in skeletal muscle tissue. Overall, our results serve to expand our knowledge of the anti-apoptotic mechanisms underlying the natural dehydration-tolerance of X. laevis, including its likely use in mitigating tissue atrophy during aestivation.