石蜡切片MDM2、MCM2、Fascin、PCNA、EGFR的免疫组织定位；在正常和癌变的人类卵巢中

Q3 Medicine

Cancer treatment and research communications Pub Date : 2025-01-01 DOI:10.1016/j.ctarc.2025.100932

Mojgan Karimi Zarchi , Mohammad Ali Khalili , Fariba Binesh , Fatemeh Pourhosseini , Ali Reza Talebi , Azam Hassanpour , Mahboubeh Vatanparast

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引用次数: 0

摘要

许多蛋白质标记物已经在人体组织中进行了研究，例如；fastin, MDM2, MCM2， EGFR和PCNA。这些标志物在细胞增殖、运动、粘附、侵袭和存活等方面具有重要的生理作用。迄今为止，一些研究人员专注于卵巢癌中的这些标志物。然而，上述标记尚未在正常人类卵巢中进行研究。利用免疫组织化学技术，我们试图在正常和癌变的卵巢组织中定位这些标记物。方法从10例正常卵巢组织和1例组织学证实的卵巢癌组织中包埋sparaffin，进行免疫组织学检查，发现上述5种标志物定位于人卵巢。首先，对每个标记的展示进行评估，然后试图量化展示的数量。结果正常卵巢组织中fastin、PCNA和MCM2含量较高，而EGFR仅局限于上皮细胞，颗粒细胞中含量极少。此外，所有正常卵巢的MDM2均为阴性。所有标记物在卵巢癌中均有不同模式的过表达。结论与正常卵巢相比，卵巢癌组织中细胞增殖和运动标志物表达过高。这些标志物可能对卵巢癌有预后价值。

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Immunohistological localization of MDM2, MCM2, Fascin, PCNA, EGFR in paraffin section; in the normal and cancerous human ovary

Background

Many protein markers have been investigated in human tissues such as; Fascin, MDM2, MCM2, EGFR, and PCNA. These markers have important physiologic roles in cell proliferation, motility, adhesion, invasion, and survival.

Aims

up to date, some researchers focused on these markers in ovarian cancer. However, the mentioned markers have not been investigated in the normal human ovary. Using the immunohistochemical technique we tried to localize these markers in the normal, and cancerous ovarian tissues.

Methods

Paraffin-embedded from 10 normal ovarian tissue and 1 case with histologically confirmed ovarian cancer, underwent an immunohistological process, and five mentioned markers were localized in the human ovary. At first, each marker presentation was assessed and then it tried to quantify the amount of presentation.

Results

Fascin, PCNA, and MCM2 are presented high in the normal ovarian tissue, while EGFR is restricted to the epithelium and very rarely in the granulosa cells. Also, MDM2 was negative for all normal ovaries. All markers had overexpression in ovarian cancer in different patterns.

Conclusion

Ovarian cancer tissue showed over-expression of the cell proliferation and motility markers compared with the normal ovary. These markers may have prognostic value in ovarian cancer.

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来源期刊

Cancer treatment and research communications Medicine-Oncology

CiteScore

4.30

自引率

0.00%

发文量

148

审稿时长

56 days

期刊介绍： Cancer Treatment and Research Communications is an international peer-reviewed publication dedicated to providing comprehensive basic, translational, and clinical oncology research. The journal is devoted to articles on detection, diagnosis, prevention, policy, and treatment of cancer and provides a global forum for the nurturing and development of future generations of oncology scientists. Cancer Treatment and Research Communications publishes comprehensive reviews and original studies describing various aspects of basic through clinical research of all tumor types. The journal also accepts clinical studies in oncology, with an emphasis on prospective early phase clinical trials. Specific areas of interest include basic, translational, and clinical research and mechanistic approaches; cancer biology; molecular carcinogenesis; genetics and genomics; stem cell and developmental biology; immunology; molecular and cellular oncology; systems biology; drug sensitivity and resistance; gene and antisense therapy; pathology, markers, and prognostic indicators; chemoprevention strategies; multimodality therapy; cancer policy; and integration of various approaches. Our mission is to be the premier source of relevant information through promoting excellence in research and facilitating the timely translation of that science to health care and clinical practice.