DNA甲基化对猪间充质干细胞脂肪细胞分化的影响及供体代谢类型的影响

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-05-01 DOI:10.1016/j.ygeno.2025.111050

Siriluck Ponsuksili , Shuaichen Li , Puntita Siengdee , Frieder Hadlich , Nares Trakooljul , Michael Oster , Henry Reyer , Klaus Wimmers

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引用次数: 0

摘要

代谢性供体间充质干细胞（MSCs）对DNA甲基化的影响是一种重要的表观遗传机制，可显著调节脂肪形成。在这项研究中，我们研究了两个代谢性能不同的猪品种（德国长白猪（DL）和安格尔马鞍背猪（AS））滑膜间充质干细胞（SMSCs）分化过程中的表观遗传变化。在体外刺激SMSCs分化为脂肪细胞时，在第7天和第14天发现了几个差异甲基化的位点和区域，特别是在基因启动子区域。AS品种，以较高的脂肪沉积而闻名，与DL相比，表现出更多的超甲基化。此外，我们利用与脂肪形成过程和品种相关的差异甲基化区域，特别是启动子区域，来预测转录因子基序。本研究提供了不同代谢类型猪脂肪形成过程中DNA甲基化景观的见解，揭示了其在培养中调节细胞命运和供体记忆保留中的作用。

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DNA methylation in adipocyte differentiation of porcine mesenchymal stem cells and the impact of the donor metabolic type

The impact of metabolic donor mesenchymal stem cells (MSCs) on DNA methylation, a critical epigenetic mechanism, significantly regulates adipogenesis. In this study, we investigated epigenetic changes during differentiation of synovial MSCs (SMSCs) from two pig breeds differing in metabolic performance (German Landrace (DL) and Angeln Saddleback (AS)). Stimulation of SMSCs to differentiate into adipocytes in vitro revealed several differentially methylated loci and regions, particularly on gene promoter regions, at day 7 and 14. AS breeds, known for higher fat deposition, exhibited more hypermethylation compared to DL. Furthermore, we utilized differentially methylated regions associated with the adipogenic process and breed, especially those in promoter regions, for predicting transcription factor motifs. This study provides insights into the DNA methylation landscape during adipogenesis in pigs of different metabolic types, revealing its role in regulating cell fate and donor memory retention in culture.

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.