Ligia Milanez Venturini , Daniella Pacheco dos Santos Haupenthal , João Afonso Spillere Dajori , Laura de Roch Casagrande , Rubya Pereira Zaccaron , Camila da Costa , Igor Ramos Lima , Fernando Russo Costa do Bomfim , Sabrina Zanchetta Lanza , Yaodong Gu , Anand Thirupathi , Paulo Cesar Lock Silveira , Alessandro Haupenthal
{"title":"脉冲超声和适度运动加速实验性胫骨骨折模型的骨愈合:生化、放射学和生物力学证据","authors":"Ligia Milanez Venturini , Daniella Pacheco dos Santos Haupenthal , João Afonso Spillere Dajori , Laura de Roch Casagrande , Rubya Pereira Zaccaron , Camila da Costa , Igor Ramos Lima , Fernando Russo Costa do Bomfim , Sabrina Zanchetta Lanza , Yaodong Gu , Anand Thirupathi , Paulo Cesar Lock Silveira , Alessandro Haupenthal","doi":"10.1016/j.bone.2025.117498","DOIUrl":null,"url":null,"abstract":"<div><div>Currently, therapeutic strategies involving mechanical stress play a key role in fracture consolidation. However, no literary evidence exists regarding the combined effects of low-intensity pulsed ultrasound (LIPUS) and moderate aerobic exercise. This study aimed to investigate the effects of LIPUS and moderate aerobic exercise on bone consolidation in 48 male Wistar rats. The animals were divided into four groups (<em>n</em> = 12 per group): Bone Fracture (BF); BF + LIPUS; BF + Exercise (Ex); BF + Ex + LIPUS. A transverse osteotomy with Kirschner wire fixation was performed, followed by therapeutic interventions for 19 days, three times a week. LIPUS was applied for 10 min (1.5 MHz, 0.4 W/cm<sup>2</sup>, 3 cm<sup>2</sup> area), and aerobic exercise lasted 30 min/day with progressive speed. Radiographic analyses were conducted on days 1, 12, and 24 post-fracture, and euthanasia occurred 72 h after the final session. The combined group exhibited improved radiographic scores and increased birefringent collagen fibers. IL-4, IL-10, and TGF-β levels were significantly higher in treated groups, particularly the combined intervention. Additionally, oxidative stress markers showed reduced nitrite levels, controlled sulfhydryl oxidation, and enhanced SOD and GSH activity. Biomechanically, the combined group tolerated higher loads and demonstrated superior deformation capacity and tissue elasticity. The combination of aerobic exercise and LIPUS enhanced radiographic healing, modulated inflammation, reduced oxidative stress, and improved mechanical properties, supporting its potential for fracture rehabilitation.</div></div>","PeriodicalId":9301,"journal":{"name":"Bone","volume":"197 ","pages":"Article 117498"},"PeriodicalIF":3.5000,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pulsed ultrasound and moderate exercise accelerate bone healing in an experimental tibial fracture model: biochemical, radiological and biomechanical evidence\",\"authors\":\"Ligia Milanez Venturini , Daniella Pacheco dos Santos Haupenthal , João Afonso Spillere Dajori , Laura de Roch Casagrande , Rubya Pereira Zaccaron , Camila da Costa , Igor Ramos Lima , Fernando Russo Costa do Bomfim , Sabrina Zanchetta Lanza , Yaodong Gu , Anand Thirupathi , Paulo Cesar Lock Silveira , Alessandro Haupenthal\",\"doi\":\"10.1016/j.bone.2025.117498\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Currently, therapeutic strategies involving mechanical stress play a key role in fracture consolidation. However, no literary evidence exists regarding the combined effects of low-intensity pulsed ultrasound (LIPUS) and moderate aerobic exercise. This study aimed to investigate the effects of LIPUS and moderate aerobic exercise on bone consolidation in 48 male Wistar rats. The animals were divided into four groups (<em>n</em> = 12 per group): Bone Fracture (BF); BF + LIPUS; BF + Exercise (Ex); BF + Ex + LIPUS. A transverse osteotomy with Kirschner wire fixation was performed, followed by therapeutic interventions for 19 days, three times a week. LIPUS was applied for 10 min (1.5 MHz, 0.4 W/cm<sup>2</sup>, 3 cm<sup>2</sup> area), and aerobic exercise lasted 30 min/day with progressive speed. Radiographic analyses were conducted on days 1, 12, and 24 post-fracture, and euthanasia occurred 72 h after the final session. The combined group exhibited improved radiographic scores and increased birefringent collagen fibers. IL-4, IL-10, and TGF-β levels were significantly higher in treated groups, particularly the combined intervention. Additionally, oxidative stress markers showed reduced nitrite levels, controlled sulfhydryl oxidation, and enhanced SOD and GSH activity. Biomechanically, the combined group tolerated higher loads and demonstrated superior deformation capacity and tissue elasticity. The combination of aerobic exercise and LIPUS enhanced radiographic healing, modulated inflammation, reduced oxidative stress, and improved mechanical properties, supporting its potential for fracture rehabilitation.</div></div>\",\"PeriodicalId\":9301,\"journal\":{\"name\":\"Bone\",\"volume\":\"197 \",\"pages\":\"Article 117498\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2025-04-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bone\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S8756328225001103\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENDOCRINOLOGY & METABOLISM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bone","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S8756328225001103","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
引用次数: 0
摘要
目前,涉及机械应力的治疗策略在骨折巩固中起着关键作用。然而,关于低强度脉冲超声(LIPUS)和适度有氧运动的联合作用,尚无文献证据。本研究旨在探讨LIPUS和适度有氧运动对48只雄性Wistar大鼠骨巩固的影响。实验动物分为4组,每组12只:骨折组(Bone Fracture, BF);bf + lipus;BF +练习(Ex);BF + Ex + LIPUS。行横截骨术加克氏针固定,治疗干预19天,每周三次。LIPUS应用10 min (1.5 MHz, 0.4 W/cm2, 3 cm2面积),有氧运动持续30 min/d,循序渐进。骨折后第1、12和24天进行影像学分析,最后一次手术后72小时进行安乐死。联合组表现出改善的x线评分和增加的双折射胶原纤维。治疗组IL-4、IL-10和TGF-β水平显著升高,特别是联合干预组。此外,氧化应激标志物显示亚硝酸盐水平降低,巯基氧化控制,SOD和GSH活性增强。生物力学方面,联合组承受更高的负荷,表现出更好的变形能力和组织弹性。有氧运动和LIPUS的结合增强了x线片愈合,调节了炎症,减少了氧化应激,改善了机械性能,支持了其骨折康复的潜力。
Pulsed ultrasound and moderate exercise accelerate bone healing in an experimental tibial fracture model: biochemical, radiological and biomechanical evidence
Currently, therapeutic strategies involving mechanical stress play a key role in fracture consolidation. However, no literary evidence exists regarding the combined effects of low-intensity pulsed ultrasound (LIPUS) and moderate aerobic exercise. This study aimed to investigate the effects of LIPUS and moderate aerobic exercise on bone consolidation in 48 male Wistar rats. The animals were divided into four groups (n = 12 per group): Bone Fracture (BF); BF + LIPUS; BF + Exercise (Ex); BF + Ex + LIPUS. A transverse osteotomy with Kirschner wire fixation was performed, followed by therapeutic interventions for 19 days, three times a week. LIPUS was applied for 10 min (1.5 MHz, 0.4 W/cm2, 3 cm2 area), and aerobic exercise lasted 30 min/day with progressive speed. Radiographic analyses were conducted on days 1, 12, and 24 post-fracture, and euthanasia occurred 72 h after the final session. The combined group exhibited improved radiographic scores and increased birefringent collagen fibers. IL-4, IL-10, and TGF-β levels were significantly higher in treated groups, particularly the combined intervention. Additionally, oxidative stress markers showed reduced nitrite levels, controlled sulfhydryl oxidation, and enhanced SOD and GSH activity. Biomechanically, the combined group tolerated higher loads and demonstrated superior deformation capacity and tissue elasticity. The combination of aerobic exercise and LIPUS enhanced radiographic healing, modulated inflammation, reduced oxidative stress, and improved mechanical properties, supporting its potential for fracture rehabilitation.
期刊介绍:
BONE is an interdisciplinary forum for the rapid publication of original articles and reviews on basic, translational, and clinical aspects of bone and mineral metabolism. The Journal also encourages submissions related to interactions of bone with other organ systems, including cartilage, endocrine, muscle, fat, neural, vascular, gastrointestinal, hematopoietic, and immune systems. Particular attention is placed on the application of experimental studies to clinical practice.