Irin rose Paul, Anaswara Sankar, Mariya Sunny, Manju Maria Mathews, Dhanish Joseph
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Skin irritancy was assessed using the HET-CAM test, while the wound healing potential was confirmed through a scratch wound healing assay.</p><h3>Results</h3><p>The formulated lip balm exhibited a pleasant appearance, uniform distribution, and was free from grittiness. Antimicrobial studies demonstrated significant antibacterial activity and promising antifungal properties against angular cheilitis-causing pathogens. The HET-CAM test confirmed that the lip balm was free from skin irritancy. Safety was further verified through in vitro cytotoxicity testing via the MTT assay, with an LC50 value of 299.957 µg/ml. High cell viability, even at 100 µg/ml, indicating no toxicity in cultured cell lines. The Scratch assay confirmed the lip balm's wound healing potential, reinforcing the formulation's efficacy.</p><h3>Conclusion</h3><p>The formulation was found to be safe and effective, suggesting its potential as a therapeutic option for angular cheilitis. 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引用次数: 0
摘要
目的研制一种治疗唇炎的中药润唇膏，并对其疗效进行评价。角性唇炎表现为嘴角一侧或更常见的嘴角两侧出现大致呈三角形的红斑和水肿。成人角状口唇炎最常见的原因是由白色念珠菌引起的真菌感染，而不太常见的是金黄色葡萄球菌。方法采用索氏提取法提取植物提取物，并采用实验设计（Design of Experiments， DOE）优化最终配方的浓度。进行了感官评价和抗菌研究。采用MTT法进行体外细胞毒性研究。使用ht - cam测试评估皮肤刺激性，而通过划痕伤口愈合试验确认伤口愈合潜力。结果配制的润唇膏外观美观，分布均匀，无砂粒。抗菌研究显示了显著的抗菌活性和有希望的抗真菌特性，对角唇炎引起的病原体。HET-CAM测试证实，唇膏没有皮肤刺激。通过MTT法体外细胞毒性试验进一步验证其安全性，LC50值为299.957µg/ml。即使浓度为100µg/ml，细胞活力也很高，表明对培养的细胞系没有毒性。划痕试验证实了唇膏的伤口愈合潜力，加强了配方的功效。结论该制剂安全有效，具有治疗角性口唇炎的潜力。这项研究提供了一个系统的方法来开发草药润唇膏使用天然提取物已知的药用特性。
Formulation and Evaluation of Herbal Lipbalm for the Treatment of Cheilitis
Purpose
This study aimed to formulate and evaluate a herbal lip balm for the treatment of cheilitis. Angular cheilitis presents as a roughly triangular area of erythema and edema at one or, more commonly, both corners of the mouth. The most frequent cause of angular cheilitis in adults is a fungal infection caused by Candida albicans, and less frequently, Staphylococcus aureus.
Methods
Plant extracts were obtained through Soxhlet extraction, and the concentration for the final formulation was optimized using Design of Experiments (DOE). Organoleptic evaluation and antimicrobial studies were conducted. In vitro cytotoxicity studies were conducted using MTT assay. Skin irritancy was assessed using the HET-CAM test, while the wound healing potential was confirmed through a scratch wound healing assay.
Results
The formulated lip balm exhibited a pleasant appearance, uniform distribution, and was free from grittiness. Antimicrobial studies demonstrated significant antibacterial activity and promising antifungal properties against angular cheilitis-causing pathogens. The HET-CAM test confirmed that the lip balm was free from skin irritancy. Safety was further verified through in vitro cytotoxicity testing via the MTT assay, with an LC50 value of 299.957 µg/ml. High cell viability, even at 100 µg/ml, indicating no toxicity in cultured cell lines. The Scratch assay confirmed the lip balm's wound healing potential, reinforcing the formulation's efficacy.
Conclusion
The formulation was found to be safe and effective, suggesting its potential as a therapeutic option for angular cheilitis. This study offers a systematic approach to developing herbal lip balm using natural extracts known for their medicinal properties.
期刊介绍：
The Journal of Pharmaceutical Innovation (JPI), is an international, multidisciplinary peer-reviewed scientific journal dedicated to publishing high quality papers emphasizing innovative research and applied technologies within the pharmaceutical and biotechnology industries. JPI''s goal is to be the premier communication vehicle for the critical body of knowledge that is needed for scientific evolution and technical innovation, from R&D to market. Topics will fall under the following categories:
Materials science,
Product design,
Process design, optimization, automation and control,
Facilities; Information management,
Regulatory policy and strategy,
Supply chain developments ,
Education and professional development,
Journal of Pharmaceutical Innovation publishes four issues a year.
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