外部机械刺激下悬浮成纤维细胞片中机械传感复合物的动态重塑

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology and Bioengineering Pub Date : 2025-04-24 DOI:10.1002/bit.28996

Madoka Suzuki, Keiko Kawauchi, Hiroaki Machiyama, Hiroaki Hirata, Shin'ichi Ishiwata, Hideaki Fujita

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引用次数: 0

摘要

独立细胞片是再生医学和组织工程中有价值的生物材料。由于细胞片在处理过程中会经历各种机械刺激，因此了解细胞对这些刺激的反应是很重要的。在这里，我们证明了在成纤维细胞片拉伸过程中各种蛋白质定位的变化。已知这些蛋白参与机械感应。拉伸时，肌动蛋白丝与拉伸方向平行。在细胞-细胞连接处，β -连环蛋白形成簇，与积累的血管蛋白、酶合蛋白以及肌动蛋白丝共定位。p130 Crk -相关底物，已知存在于局灶黏附中，也被招募到这些簇中并磷酸化。我们的研究结果表明，当细胞片被拉伸时，机械传感机制在细胞-细胞连接处形成。

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Dynamic Remodeling of Mechano‐Sensing Complexes in Suspended Fibroblast Cell‐Sheets Under External Mechanical Stimulus

Freestanding cell‐sheets are valuable bio‐materials for use in regenerative medicine and tissue engineering. Because cell‐sheets experience various mechanical stimulations during handling, it is important to understand the responses of cells to these stimulations. Here, we demonstrate changes in the localization of various proteins during the stretching of fibroblast cell‐sheets. These proteins are known to be involved in mechano‐sensing. Upon stretching, actin filaments appear parallel to the stretching direction. At cell‐cell junctions, β‐catenin forms clusters that co‐localize with accumulated vinculin and zyxin as well as the actin filaments. The p130 Crk‐associated substrate, known to be present in focal adhesions, is also recruited to these clusters and phosphorylated. Our results suggest that mechano‐sensing machinery is formed at cell‐cell junctions when the cell‐sheets are stretched.

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来源期刊

Biotechnology and Bioengineering 工程技术-生物工程与应用微生物

CiteScore

7.90

自引率

5.30%

发文量

280

审稿时长

2.1 months

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