壳聚糖/羟丙基甲基纤维素乙酸琥珀酸盐纳米颗粒作为姜黄素在MDA-MB-231乳腺癌细胞中的一种有前景的递送系统

IF 5.3 2区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Molecular Liquids Pub Date : 2025-04-15 DOI:10.1016/j.molliq.2025.127600

Layla Schlichting , Giuliana Valentini , Bruna M. Santos , Mariele P. Sanches , Rafael V. Soares , Rodrigo H. Saatkamp , Maicon R. Kviecinski , Ariane Zamoner , Alexandre Luis Parize

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引用次数: 0

摘要

乳腺癌是一个超越国界和文化的全球公共卫生问题，影响着每一大洲的生活。黄酮类化合物如姜黄素（CUR）作为抗肿瘤药物已经得到了突出的应用。然而，姜黄素的一些理化特性限制了它的功效。由于这个原因，新的策略，如聚合纳米粒子（pNPs），已经被开发出来，以提高姜黄素在水中的溶解度，并防止其降解。本研究采用离子凝胶法制备了不含（HPMCAS@CTS）和含姜黄素（HPMCAS@CTS-CUR）的羟丙基甲基纤维素乙酸琥珀酸酯（HPMCAS）、壳聚糖（CTS）和壳聚糖（CTS），通过不同的成分和搅拌时间制备了用于乳腺癌治疗的pNPs。姜黄素的加入增加了NPs的平均直径（156.4±1 ~ 206±1 nm），降低了NPs的多分散性（0.206 ~ 0.180）。透射电镜显示纳米结构呈圆形。量热分析和光谱分析表明，CUR成功地结合到聚合物基体中，包封量为0.23 mg mL−1，包封效率为99.97%。HPMCAS@CTS-CUR纳米颗粒对MDA-MB-231人乳腺癌细胞系的细胞毒性比游离CUR强。此外，在菌落形成单位（CFU）测定中，CFU的数量减少。此外，纳米颗粒配方抑制细胞迁移，突出了其提高CUR对乳腺肿瘤的治疗效果和防止转移的潜力。这些发现突出了纳米颗粒递送系统在提高CUR抗肿瘤性能方面的潜力。

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Chitosan/hydroxypropyl methylcellulose acetate succinate nanoparticles as a promising delivery system for curcumin in MDA-MB-231 breast cancer cells

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Chitosan/hydroxypropyl methylcellulose acetate succinate nanoparticles as a promising delivery system for curcumin in MDA-MB-231 breast cancer cells

Breast cancer is a global public health problem that transcends borders and cultures, impacting lives on every continent. Flavonoids such as curcumin (CUR) have gained prominence as anti-tumor agents. However, some of curcumin’s physicochemical features limit its efficacy. For this reason, new strategies, such as polymeric nanoparticles (pNPs), have been developed to increase curcumin’s solubility in water and protect it from degradation. In this study, pNPs composed of hydroxypropylmethylcellulose acetate succinate (HPMCAS), chitosan (CTS) without (HPMCAS@CTS) and with curcumin (HPMCAS@CTS-CUR), were produced by the ionic gelation method, trying different compositions and stirring times, for breast cancer treatment. The addition of curcumin showed an increase in the average diameter of the NPs (156.4 ± 1 to 206 ± 1 nm), and a decrease in polydispersity (0.206 to 0.180). Nanostructures demonstrated a round-shaped profile by TEM. Calorimetry and spectroscopy analyses suggested the successful incorporation of CUR into the polymer matrix, with a total of 0.23 mg mL⁻¹ encapsulated and an efficiency of 99.97 %. HPMCAS@CTS-CUR nanoparticles presented strongly increased cytotoxicity against the MDA-MB-231 human breast cancer cell line compared to free CUR. Additionally, in the colony forming units (CFU) assay, the number of CFUs decreased. Furthermore, the nanoparticle formulation inhibited cell migration, highlighting its potential to improve CUR’s therapeutic efficacy against breast tumors and prevent metastasis. These findings highlight the potential of the nanoparticle delivery system in improving the antitumor performance of CUR.

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来源期刊

Journal of Molecular Liquids 化学-物理：原子、分子和化学物理

CiteScore

10.30

自引率

16.70%

发文量

2597

审稿时长

78 days

期刊介绍： The journal includes papers in the following areas: – Simple organic liquids and mixtures – Ionic liquids – Surfactant solutions (including micelles and vesicles) and liquid interfaces – Colloidal solutions and nanoparticles – Thermotropic and lyotropic liquid crystals – Ferrofluids – Water, aqueous solutions and other hydrogen-bonded liquids – Lubricants, polymer solutions and melts – Molten metals and salts – Phase transitions and critical phenomena in liquids and confined fluids – Self assembly in complex liquids.– Biomolecules in solution The emphasis is on the molecular (or microscopic) understanding of particular liquids or liquid systems, especially concerning structure, dynamics and intermolecular forces. The experimental techniques used may include: – Conventional spectroscopy (mid-IR and far-IR, Raman, NMR, etc.) – Non-linear optics and time resolved spectroscopy (psec, fsec, asec, ISRS, etc.) – Light scattering (Rayleigh, Brillouin, PCS, etc.) – Dielectric relaxation – X-ray and neutron scattering and diffraction. Experimental studies, computer simulations (MD or MC) and analytical theory will be considered for publication; papers just reporting experimental results that do not contribute to the understanding of the fundamentals of molecular and ionic liquids will not be accepted. Only papers of a non-routine nature and advancing the field will be considered for publication.